产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
表皮生长因子受体单克隆抗体(表皮生长因子受体.1), 生物素
目录 :
MA5-13045
规格 :
500微升
价格 :
美国405.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
生物素
克隆名称 :
表皮生长因子受体.1
反应物种 :
人类, 国内马
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-冰冻切片, 抑制或激活实验
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 24
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Oksvold M, Pedersen N, Forfang L, Smeland E. Effect of cycloheximide on epidermal growth factor receptor trafficking and signaling. FEBS Lett. 2012;586:3575-81 pubmed 出版商
  • 免疫沉淀; 人类
Freeman D, McDorman K, Ogbagabriel S, Kozlosky C, Yang B, Doshi S, et al. Tumor penetration and epidermal growth factor receptor saturation by panitumumab correlate with antitumor activity in a preclinical model of human cancer. Mol Cancer. 2012;11:47 pubmed 出版商
  • 抑制或激活实验; 人类
Minai L, Yeheskely Hayon D, Golan L, Bisker G, Dann E, Yelin D. Optical nanomanipulations of malignant cells: controlled cell damage and fusion. Small. 2012;8:1732-9 pubmed 出版商
  • 免疫细胞化学; 人类
  • 免疫组化; 人类
Bertelsen V, Sak M, Breen K, Rødland M, Johannessen L, Traub L, et al. A chimeric pre-ubiquitinated EGF receptor is constitutively endocytosed in a clathrin-dependent, but kinase-independent manner. Traffic. 2011;12:507-20 pubmed 出版商
  • 免疫细胞化学; 人类; 1:100
Zeineldin R, Ning Y, Hudson L. The constitutive activity of epidermal growth factor receptor vIII leads to activation and differential trafficking of wild-type epidermal growth factor receptor and erbB2. J Histochem Cytochem. 2010;58:529-41 pubmed 出版商
  • 流式细胞仪; 人类
Wegner C, Wegener C, Malerød L, Pedersen N, Progida C, Prodiga C, et al. Ultrastructural characterization of giant endosomes induced by GTPase-deficient Rab5. Histochem Cell Biol. 2010;133:41-55 pubmed 出版商
  • 流式细胞仪; 人类
Red Brewer M, Choi S, Alvarado D, Moravcevic K, Pozzi A, Lemmon M, et al. The juxtamembrane region of the EGF receptor functions as an activation domain. Mol Cell. 2009;34:641-51 pubmed 出版商
  • 流式细胞仪; 人类
Pedersen N, Breen K, Rødland M, Haslekås C, Stang E, Madshus I. Expression of epidermal growth factor receptor or ErbB3 facilitates geldanamycin-induced down-regulation of ErbB2. Mol Cancer Res. 2009;7:275-84 pubmed 出版商
  • 免疫细胞化学; 人类
Barr D, Ostermeyer Fay A, Matundan R, Brown D. Clathrin-independent endocytosis of ErbB2 in geldanamycin-treated human breast cancer cells. J Cell Sci. 2008;121:3155-66 pubmed 出版商
  • 流式细胞仪; 人类; 10 mg/ml
Offterdinger M, Bastiaens P. Prolonged EGFR signaling by ERBB2-mediated sequestration at the plasma membrane. Traffic. 2008;9:147-55 pubmed
  • 免疫组化; 人类; 1:25
Goldstein N, Decker D, Severson D, Schell S, Vicini F, Margolis J, et al. Molecular classification system identifies invasive breast carcinoma patients who are most likely and those who are least likely to achieve a complete pathologic response after neoadjuvant chemotherapy. Cancer. 2007;110:1687-96 pubmed
  • 免疫细胞化学; 人类
Sundelin K, Roberg K, Grenman R, Hakansson L. Effects of cisplatin, alpha-interferon, and 13-cis retinoic acid on the expression of Fas (CD95), intercellular adhesion molecule-1 (ICAM-1), and epidermal growth factor receptor (EGFR) in oral cancer cell lines. J Oral Pathol Med. 2007;36:177-83 pubmed
  • 免疫细胞化学; 人类
Myromslien F, Grøvdal L, Raiborg C, Stenmark H, Madshus I, Stang E. Both clathrin-positive and -negative coats are involved in endosomal sorting of the EGF receptor. Exp Cell Res. 2006;312:3036-48 pubmed
  • 免疫细胞化学; 人类
Johannessen L, Pedersen N, Pedersen K, Madshus I, Stang E. Activation of the epidermal growth factor (EGF) receptor induces formation of EGF receptor- and Grb2-containing clathrin-coated pits. Mol Cell Biol. 2006;26:389-401 pubmed
  • 免疫细胞化学; 人类
Haslekås C, Breen K, Pedersen K, Johannessen L, Stang E, Madshus I. The inhibitory effect of ErbB2 on epidermal growth factor-induced formation of clathrin-coated pits correlates with retention of epidermal growth factor receptor-ErbB2 oligomeric complexes at the plasma membrane. Mol Biol Cell. 2005;16:5832-42 pubmed
  • 免疫细胞化学; 人类
Locke M, Heywood M, Fawell S, Mackenzie I. Retention of intrinsic stem cell hierarchies in carcinoma-derived cell lines. Cancer Res. 2005;65:8944-50 pubmed
  • 免疫细胞化学; 人类
Longva K, Pedersen N, Haslekås C, Stang E, Madshus I. Herceptin-induced inhibition of ErbB2 signaling involves reduced phosphorylation of Akt but not endocytic down-regulation of ErbB2. Int J Cancer. 2005;116:359-67 pubmed
  • 免疫组化; 人类; 1:100
Odajima T, Sasaki Y, Tanaka N, Kato Mori Y, Asanuma H, Ikeda T, et al. Abnormal beta-catenin expression in oral cancer with no gene mutation: correlation with expression of cyclin D1 and epidermal growth factor receptor, Ki-67 labeling index, and clinicopathological features. Hum Pathol. 2005;36:234-41 pubmed
  • 免疫组化; 人类; 1:200
Lo H, Xia W, Wei Y, Ali Seyed M, Huang S, Hung M. Novel prognostic value of nuclear epidermal growth factor receptor in breast cancer. Cancer Res. 2005;65:338-48 pubmed
Grøvdal L, Stang E, Sorkin A, Madshus I. Direct interaction of Cbl with pTyr 1045 of the EGF receptor (EGFR) is required to sort the EGFR to lysosomes for degradation. Exp Cell Res. 2004;300:388-95 pubmed
Witton C, Reeves J, Going J, Cooke T, Bartlett J. Expression of the HER1-4 family of receptor tyrosine kinases in breast cancer. J Pathol. 2003;200:290-7 pubmed
Yu X, Sharma K, Takahashi T, Iwamoto R, Mekada E. Ligand-independent dimer formation of epidermal growth factor receptor (EGFR) is a step separable from ligand-induced EGFR signaling. Mol Biol Cell. 2002;13:2547-57 pubmed
Ge H, Gong X, Tang C. Evidence of high incidence of EGFRvIII expression and coexpression with EGFR in human invasive breast cancer by laser capture microdissection and immunohistochemical analysis. Int J Cancer. 2002;98:357-61 pubmed
O Bryan J, Lambert Q, Der C. The src homology 2 and phosphotyrosine binding domains of the ShcC adaptor protein function as inhibitors of mitogenic signaling by the epidermal growth factor receptor. J Biol Chem. 1998;273:20431-7 pubmed
图像
图像 1 :
赛默飞世尔 MA5-13045 图像 1
产品信息
产品类型 :
抗体
产品名称 :
表皮生长因子受体单克隆抗体(表皮生长因子受体.1), 生物素
目录# :
MA5-13045
规格 :
500微升
价格 :
美国405.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Equine, Human
应用 :
Flow Cytometry: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: 2 µg/mL
物种 :
Equine, Human
克隆 :
表皮生长因子受体.1
抗体亚型 :
IgG2b, kappa
储存 :
4° C
描述 :
EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
免疫原 :
A431 human epidermoid cells
格式 :
液体
应用w/稀释 :
Flow Cytometry: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Immunoprecipitation: 2 µg/mL
别名 :
2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国