产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
表皮生长因子受体单克隆抗体(H11)
目录 :
MA5-13070
规格 :
500微升
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
H11
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片, 抑制或激活实验
更多信息或购买 :
文章摘录数: 57
出版应用/物种/样本/稀释参考文献
  • 免疫组化; 人类; 1:200; 图 6a
  • 免疫印迹; 人类; 图 1b
Engel B, Bowser J, Broaddus R, Carson D. MUC1 stimulates EGFR expression and function in endometrial cancer. Oncotarget. 2016;7:32796-809 pubmed 出版商
  • 免疫细胞化学; 人类; 1:200
Engel B, Constantinou P, Sablatura L, Doty N, Carson D, Farach Carson M, et al. Multilayered, Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures. Adv Healthc Mater. 2015;4:1664-74 pubmed 出版商
  • 免疫沉淀; 人类
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Kyriakakis E, Maslova K, Frachet A, Ferri N, Contini A, Pfaff D, et al. Cross-talk between EGFR and T-cadherin: EGFR activation promotes T-cadherin localization to intercellular contacts. Cell Signal. 2013;25:1044-53 pubmed 出版商
  • 免疫细胞化学; 人类
Gupta A, Mehta R, Alimirah F, Peng X, Murillo G, WIEHLE R, et al. Efficacy and mechanism of action of Proellex, an antiprogestin in aromatase overexpressing and Letrozole resistant T47D breast cancer cells. J Steroid Biochem Mol Biol. 2013;133:30-42 pubmed 出版商
  • 抑制或激活实验; 人类
Spangler J, Manzari M, Rosalia E, Chen T, Wittrup K. Triepitopic antibody fusions inhibit cetuximab-resistant BRAF and KRAS mutant tumors via EGFR signal repression. J Mol Biol. 2012;422:532-44 pubmed 出版商
  • 免疫印迹; 人类
Collins D, O Donovan N, McGowan P, O SULLIVAN F, Duffy M, Crown J. Trastuzumab induces antibody-dependent cell-mediated cytotoxicity (ADCC) in HER-2-non-amplified breast cancer cell lines. Ann Oncol. 2012;23:1788-95 pubmed 出版商
  • 免疫印迹; 人类
Cho J, Pastorino S, Zeng Q, Xu X, Johnson W, Vandenberg S, et al. Glioblastoma-derived epidermal growth factor receptor carboxyl-terminal deletion mutants are transforming and are sensitive to EGFR-directed therapies. Cancer Res. 2011;71:7587-96 pubmed 出版商
  • 免疫印迹; 人类; 1:2000
Hanabata Y, Nakajima Y, Morita K, Kayamori K, Omura K. Coexpression of SGLT1 and EGFR is associated with tumor differentiation in oral squamous cell carcinoma. Odontology. 2012;100:156-63 pubmed 出版商
  • 免疫沉淀; 人类
DeFazio Eli L, Strommen K, Dao Pick T, Parry G, Goodman L, Winslow J. Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action. Breast Cancer Res. 2011;13:R44 pubmed 出版商
  • 免疫细胞化学; 人类; 2.5 ug/ml
Eifler R, Lind J, Falkenhagen D, Weber V, Fischer M, Zeillinger R. Enrichment of circulating tumor cells from a large blood volume using leukapheresis and elutriation: proof of concept. Cytometry B Clin Cytom. 2011;80:100-11 pubmed 出版商
  • 免疫印迹; 人类; 1:500
Sethi S, Radio N, Kotlarczyk M, Chen C, Wei Y, Jockers R, et al. Determination of the minimal melatonin exposure required to induce osteoblast differentiation from human mesenchymal stem cells and these effects on downstream signaling pathways. J Pineal Res. 2010;49:222-38 pubmed 出版商
  • 流式细胞仪; 人类; 1:800
  • 免疫细胞化学; 人类; 1:500, 1:800
  • 免疫印迹; 人类
Farabegoli F, Papi A, Orlandi M. (-)-Epigallocatechin-3-gallate down-regulates EGFR, MMP-2, MMP-9 and EMMPRIN and inhibits the invasion of MCF-7 tamoxifen-resistant cells. Biosci Rep. 2011;31:99-108 pubmed 出版商
  • 抑制或激活实验; 人类; 1 mg/ml
Lowe A, Ozer B, Bai Y, Bertics P, Abbott N. Design of surfaces for liquid crystal-based bioanalytical assays. ACS Appl Mater Interfaces. 2010;2:722-31 pubmed 出版商
  • 免疫印迹; 人类
Yamada S, Yanamoto S, Kawasaki G, Mizuno A, Nemoto T. Overexpression of cortactin increases invasion potential in oral squamous cell carcinoma. Pathol Oncol Res. 2010;16:523-31 pubmed 出版商
  • 免疫组化; 小鼠; 1:500
Ozer B, Wiepz G, Bertics P. Activity and cellular localization of an oncogenic glioblastoma multiforme-associated EGF receptor mutant possessing a duplicated kinase domain. Oncogene. 2010;29:855-64 pubmed 出版商
  • 免疫印迹; 人类
Cetin Z, Ozbilim G, Erdogan A, Luleci G, Karauzum S. Evaluation of PTEN and Mcl-1 expressions in NSCLC expressing wild-type or mutated EGFR. Med Oncol. 2010;27:853-60 pubmed 出版商
  • 免疫印迹; 人类
Adam L, Zhong M, Choi W, Qi W, Nicoloso M, Arora A, et al. miR-200 expression regulates epithelial-to-mesenchymal transition in bladder cancer cells and reverses resistance to epidermal growth factor receptor therapy. Clin Cancer Res. 2009;15:5060-72 pubmed 出版商
  • 免疫印迹; 人类
Schicher N, Paulitschke V, Swoboda A, Kunstfeld R, Loewe R, Pilarski P, et al. Erlotinib and bevacizumab have synergistic activity against melanoma. Clin Cancer Res. 2009;15:3495-502 pubmed 出版商
  • 免疫印迹; 人类
Chen Y, Chang C, Hsu H, Chiou S, Lee L, Hseu T. Sennoside B inhibits PDGF receptor signaling and cell proliferation induced by PDGF-BB in human osteosarcoma cells. Life Sci. 2009;84:915-22 pubmed 出版商
  • 流式细胞仪; 人类
  • 免疫组化; 人类
  • 免疫印迹; 人类
Al Nedawi K, Meehan B, Kerbel R, Allison A, Rak J. Endothelial expression of autocrine VEGF upon the uptake of tumor-derived microvesicles containing oncogenic EGFR. Proc Natl Acad Sci U S A. 2009;106:3794-9 pubmed 出版商
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Corkery B, Crown J, Clynes M, O Donovan N. Epidermal growth factor receptor as a potential therapeutic target in triple-negative breast cancer. Ann Oncol. 2009;20:862-7 pubmed 出版商
  • 免疫印迹; 人类
Graner M, Alzate O, Dechkovskaia A, Keene J, Sampson J, Mitchell D, et al. Proteomic and immunologic analyses of brain tumor exosomes. FASEB J. 2009;23:1541-57 pubmed 出版商
  • 免疫沉淀; 人类
Lowe A, Ozer B, Wiepz G, Bertics P, Abbott N. Engineering of PDMS surfaces for use in microsystems for capture and isolation of complex and biomedically important proteins: epidermal growth factor receptor as a model system. Lab Chip. 2008;8:1357-64 pubmed 出版商
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Guix M, Faber A, Wang S, Olivares M, Song Y, Qu S, et al. Acquired resistance to EGFR tyrosine kinase inhibitors in cancer cells is mediated by loss of IGF-binding proteins. J Clin Invest. 2008;118:2609-19 pubmed 出版商
  • 免疫印迹; 人类; 1:1000
Wu Y, Li H, Lv X, Wei Q, Li X, Liu X, et al. Overexpression of JKTBP1 induces androgen-independent LNCaP cell proliferation through activation of epidermal growth factor-receptor (EGF-R). Cell Biochem Funct. 2008;26:467-77 pubmed 出版商
  • 免疫印迹; 人类; 1:200-1000
Black P, Brown G, Inamoto T, Shrader M, Arora A, Siefker Radtke A, et al. Sensitivity to epidermal growth factor receptor inhibitor requires E-cadherin expression in urothelial carcinoma cells. Clin Cancer Res. 2008;14:1478-86 pubmed 出版商
  • 抑制或激活实验; 人类
  • 免疫印迹; 人类
Sakaguchi M, Murata H, Sonegawa H, Sakaguchi Y, Futami J, Kitazoe M, et al. Truncation of annexin A1 is a regulatory lever for linking epidermal growth factor signaling with cytosolic phospholipase A2 in normal and malignant squamous epithelial cells. J Biol Chem. 2007;282:35679-86 pubmed
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Li X, Wei J, Tai H. Activation of extracellular signal-regulated kinase by 12-hydroxyheptadecatrienoic acid in prostate cancer PC3 cells. Arch Biochem Biophys. 2007;467:20-30 pubmed
  • 免疫印迹; 小鼠; 1:2000
Blair J, Rauh D, Kung C, Yun C, Fan Q, Rode H, et al. Structure-guided development of affinity probes for tyrosine kinases using chemical genetics. Nat Chem Biol. 2007;3:229-38 pubmed
  • 免疫印迹; 人类; 1:200
Roberg K, Jonsson A, Grenman R, Norberg Spaak L. Radiotherapy response in oral squamous carcinoma cell lines: evaluation of apoptotic proteins as prognostic factors. Head Neck. 2007;29:325-34 pubmed
  • 免疫印迹; 人类
Qiu Q, Domarkas J, Banerjee R, Katsoulas A, McNamee J, Jean Claude B. Type II combi-molecules: design and binary targeting properties of the novel triazolinium-containing molecules JDD36 and JDE05. Anticancer Drugs. 2007;18:171-7 pubmed
  • 免疫细胞化学; 小鼠
Choi S, Mendrola J, Lemmon M. EGF-independent activation of cell-surface EGF receptors harboring mutations found in gefitinib-sensitive lung cancer. Oncogene. 2007;26:1567-76 pubmed
  • 免疫印迹; 人类
Franovic A, Robert I, Smith K, Kurban G, Pause A, Gunaratnam L, et al. Multiple acquired renal carcinoma tumor capabilities abolished upon silencing of ADAM17. Cancer Res. 2006;66:8083-90 pubmed
  • 流式细胞仪; 人类; 1:100
  • 免疫细胞化学; 人类; 1:20
Chen Z, Mok H, Pflugfelder S, Li D, Barry M. Improved transduction of human corneal epithelial progenitor cells with cell-targeting adenoviral vectors. Exp Eye Res. 2006;83:798-806 pubmed
  • 免疫印迹; 人类
Fan Q, Knight Z, Goldenberg D, Yu W, Mostov K, Stokoe D, et al. A dual PI3 kinase/mTOR inhibitor reveals emergent efficacy in glioma. Cancer Cell. 2006;9:341-9 pubmed
  • 免疫印迹; 人类
Damiano V, Caputo R, Bianco R, D Armiento F, Leonardi A, De Placido S, et al. Novel toll-like receptor 9 agonist induces epidermal growth factor receptor (EGFR) inhibition and synergistic antitumor activity with EGFR inhibitors. Clin Cancer Res. 2006;12:577-83 pubmed
  • 免疫印迹; 人类
Márquez D, Chen H, Curran E, Welshons W, Pietras R. Estrogen receptors in membrane lipid rafts and signal transduction in breast cancer. Mol Cell Endocrinol. 2006;246:91-100 pubmed
  • 免疫印迹; 人类; 1:3000
Kassouf W, Dinney C, Brown G, McConkey D, Diehl A, Bar Eli M, et al. Uncoupling between epidermal growth factor receptor and downstream signals defines resistance to the antiproliferative effect of Gefitinib in bladder cancer cells. Cancer Res. 2005;65:10524-35 pubmed
  • 免疫印迹; 人类; 1:50
Saydam O, Glauser D, Heid I, Turkeri G, Hilbe M, Jacobs A, et al. Herpes simplex virus 1 amplicon vector-mediated siRNA targeting epidermal growth factor receptor inhibits growth of human glioma cells in vivo. Mol Ther. 2005;12:803-12 pubmed
  • 酶联免疫吸附测定; 人类; 表 2
Traxler P, Allegrini P, Brandt R, Brueggen J, Cozens R, Fabbro D, et al. AEE788: a dual family epidermal growth factor receptor/ErbB2 and vascular endothelial growth factor receptor tyrosine kinase inhibitor with antitumor and antiangiogenic activity. Cancer Res. 2004;64:4931-41 pubmed
  • 酶联免疫吸附测定; 人类; 50 ng/well; 表 1
Garcia Echeverria C, Pearson M, Marti A, Meyer T, Mestan J, Zimmermann J, et al. In vivo antitumor activity of NVP-AEW541-A novel, potent, and selective inhibitor of the IGF-IR kinase. Cancer Cell. 2004;5:231-9 pubmed
  • 免疫印迹; 人类; 图 2
Pedersen M, Tkach V, Pedersen N, Berezin V, Poulsen H. Expression of a naturally occurring constitutively active variant of the epidermal growth factor receptor in mouse fibroblasts increases motility. Int J Cancer. 2004;108:643-53 pubmed
Grindel B, Engel B, Hall C, Kelderhouse L, Lucci A, Zacharias N, et al. Mammalian Expression and In Situ Biotinylation of Extracellular Protein Targets for Directed Evolution. ACS Omega. 2020;5:25440-25455 pubmed 出版商
Al Akhrass H, Naves T, Vincent F, Magnaudeix A, Durand K, Bertin F, et al. Sortilin limits EGFR signaling by promoting its internalization in lung cancer. Nat Commun. 2017;8:1182 pubmed 出版商
Dragowska W, Warburton C, Yapp D, Minchinton A, Hu Y, Waterhouse D, et al. HER-2/neu overexpression increases the viable hypoxic cell population within solid tumors without causing changes in tumor vascularization. Mol Cancer Res. 2004;2:606-19 pubmed
Lammering G, Hewit T, Holmes M, Valerie K, Hawkins W, Lin P, et al. Inhibition of the type III epidermal growth factor receptor variant mutant receptor by dominant-negative EGFR-CD533 enhances malignant glioma cell radiosensitivity. Clin Cancer Res. 2004;10:6732-43 pubmed
Matheson S, McNamee J, Wang T, Alaoui Jamali M, Tari A, Jean Claude B. The combi-targeting concept: dissection of the binary mechanism of action of the combi-triazene SMA41 in vitro and antitumor activity in vivo. J Pharmacol Exp Ther. 2004;311:1163-70 pubmed
Cochran J, Kim Y, Olsen M, Bhandari R, Wittrup K. Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments. J Immunol Methods. 2004;287:147-58 pubmed
Fan Q, Specht K, Zhang C, Goldenberg D, Shokat K, Weiss W. Combinatorial efficacy achieved through two-point blockade within a signaling pathway-a chemical genetic approach. Cancer Res. 2003;63:8930-8 pubmed
Planque S, Taguchi H, Burr G, Bhatia G, Karle S, Zhou Y, et al. Broadly distributed chemical reactivity of natural antibodies expressed in coordination with specific antigen binding activity. J Biol Chem. 2003;278:20436-43 pubmed
Gilmour L, MacLeod K, McCaig A, Sewell J, Gullick W, Smyth J, et al. Neuregulin expression, function, and signaling in human ovarian cancer cells. Clin Cancer Res. 2002;8:3933-42 pubmed
Brahimi F, Matheson S, Dudouit F, McNamee J, Tari A, Jean Claude B. Inhibition of epidermal growth factor receptor-mediated signaling by "Combi-triazene" BJ2000, a new probe for Combi-Targeting postulates. J Pharmacol Exp Ther. 2002;303:238-46 pubmed
Damstrup L, Wandahl Pedersen M, Bastholm L, Elling F, Skovgaard Poulsen H. Epidermal growth factor receptor mutation type III transfected into a small cell lung cancer cell line is predominantly localized at the cell surface and enhances the malignant phenotype. Int J Cancer. 2002;97:7-14 pubmed
Lammering G, Lin P, Contessa J, Hampton J, Valerie K, Schmidt Ullrich R. Adenovirus-mediated overexpression of dominant negative epidermal growth factor receptor-CD533 as a gene therapeutic approach radiosensitizes human carcinoma and malignant glioma cells. Int J Radiat Oncol Biol Phys. 2001;51:775-84 pubmed
Charvat S, Chignol M, Souchier C, Le Griel C, Schmitt D, Serres M. Cell migration and MMP-9 secretion are increased by epidermal growth factor in HaCaT-ras transfected cells. Exp Dermatol. 1998;7:184-90 pubmed
Bandyopadhyay D, Mandal M, Adam L, Mendelsohn J, Kumar R. Physical interaction between epidermal growth factor receptor and DNA-dependent protein kinase in mammalian cells. J Biol Chem. 1998;273:1568-73 pubmed
Wikstrand C, Hale L, Batra S, Hill M, Humphrey P, Kurpad S, et al. Monoclonal antibodies against EGFRvIII are tumor specific and react with breast and lung carcinomas and malignant gliomas. Cancer Res. 1995;55:3140-8 pubmed
图像
图像 1 :
赛默飞世尔 MA5-13070 图像 1
图像 2 :
赛默飞世尔 MA5-13070 图像 2
Formalin-fixed, paraffin-embedded human squamous cell carcinoma of lung stained with EGFR antibody using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of tumor cells.
图像 3 :
赛默飞世尔 MA5-13070 图像 3
产品信息
产品类型 :
抗体
产品名称 :
表皮生长因子受体单克隆抗体(H11)
目录# :
MA5-13070
规格 :
500微升
价格 :
美国425.00
克隆性 :
单克隆
纯度 :
蛋白质G
宿主 :
小鼠
反应物种 :
人类, 小鼠
应用 :
Flow Cytometry: 0.5-1 µg/test, Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): 2-4 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 0.5-1.0 µg/mL
物种 :
人类, 小鼠
克隆 :
H11
抗体亚型 :
IgG1, kappa
储存 :
4° C
描述 :
EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
免疫原 :
HC2 20 d2细胞
格式 :
液体
应用w/稀释 :
Flow Cytometry: 0.5-1 µg/test, Immunocytochemistry: 2 µg/mL, Immunohistochemistry (Paraffin): 2-4 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 0.5-1.0 µg/mL
别名 :
2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国