产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
表皮生长因子受体单克隆抗体(199.12)
目录 :
MA5-13319
规格 :
500微升
价格 :
美国425.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
199.12
反应物种 :
African green monkey, 人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 染色质免疫沉淀 , 抑制或激活实验
更多信息或购买 :
文章摘录数: 61
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 1:100; 图 6s1a
Lyashenko E, Niepel M, Dixit P, Lim S, Sorger P, Vitkup D. Receptor-based mechanism of relative sensing and cell memory in mammalian signaling networks. elife. 2020;9: pubmed 出版商
  • 免疫细胞化学; 人类; 图 s5a
Kadlecova Z, Spielman S, Loerke D, Mohanakrishnan A, Reed D, Schmid S. Regulation of clathrin-mediated endocytosis by hierarchical allosteric activation of AP2. J Cell Biol. 2017;216:167-179 pubmed 出版商
  • 免疫细胞化学; 人类; 1:1000; 图 s6
Truong D, Puleo J, Llave A, Mouneimne G, Kamm R, Nikkhah M. Breast Cancer Cell Invasion into a Three Dimensional Tumor-Stroma Microenvironment. Sci Rep. 2016;6:34094 pubmed 出版商
  • 抑制或激活实验; 人类; 图 1c
Elkin S, Oswald N, Reed D, Mettlen M, Macmillan J, Schmid S. Ikarugamycin: A Natural Product Inhibitor of Clathrin-Mediated Endocytosis. Traffic. 2016;17:1139-49 pubmed 出版商
  • 免疫细胞化学; 人类; 1:1000
Zhou J, Joshi B, Duan X, Pant A, Qiu Z, Kuick R, et al. EGFR Overexpressed in Colonic Neoplasia Can be Detected on Wide-Field Endoscopic Imaging. Clin Transl Gastroenterol. 2015;6:e101 pubmed 出版商
  • 免疫沉淀; 人类; 0.8 ug/time
  • 免疫印迹; 人类
Hasegawa Y, Takahashi M, Ariki S, Asakawa D, Tajiri M, Wada Y, et al. Surfactant protein D suppresses lung cancer progression by downregulation of epidermal growth factor signaling. Oncogene. 2015;34:838-45 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
Chou C, Lee H, Tsou P, Chen C, Hsu J, Yamaguchi H, et al. mMAPS: a flow-proteometric technique to analyze protein-protein interactions in individual signaling complexes. Sci Signal. 2014;7:rs1 pubmed 出版商
  • 抑制或激活实验; 人类
Otero C, Linke M, Sanchez P, Gonzalez A, Schaap I. Propranolol restricts the mobility of single EGF-receptors on the cell surface before their internalization. PLoS ONE. 2013;8:e83086 pubmed 出版商
  • 免疫细胞化学; 人类
Henriksen L, Grandal M, Knudsen S, van Deurs B, Grøvdal L. Internalization mechanisms of the epidermal growth factor receptor after activation with different ligands. PLoS ONE. 2013;8:e58148 pubmed 出版商
  • 染色质免疫沉淀 ; 人类
Han W, Carpenter R, Cao X, Lo H. STAT1 gene expression is enhanced by nuclear EGFR and HER2 via cooperation with STAT3. Mol Carcinog. 2013;52:959-69 pubmed 出版商
  • 酶联免疫吸附测定; 人类; 0.3 ug/ml
Schwenk J, Poetz O, Zeillinger R, Joos T. A Miniaturized Ligand Binding Assay for EGFR. Int J Proteomics. 2012;2012:247059 pubmed 出版商
  • 免疫沉淀; 人类; 1.5 ug
Siegemund M, Pollak N, Seifert O, Wahl K, Hanak K, Vogel A, et al. Superior antitumoral activity of dimerized targeted single-chain TRAIL fusion proteins under retention of tumor selectivity. Cell Death Dis. 2012;3:e295 pubmed 出版商
  • 免疫细胞化学; 人类
Rong G, Reinhard B. Monitoring the size and lateral dynamics of ErbB1 enriched membrane domains through live cell plasmon coupling microscopy. PLoS ONE. 2012;7:e34175 pubmed 出版商
  • 流式细胞仪; 人类
  • 免疫沉淀; 人类
  • 免疫细胞化学; 人类
Grandal M, Grøvdal L, Henriksen L, Andersen M, Holst M, Madshus I, et al. Differential roles of Grb2 and AP-2 in p38 MAPK- and EGF-induced EGFR internalization. Traffic. 2012;13:576-85 pubmed 出版商
  • 抑制或激活实验; 人类; 100 ug/ml
Pötz O, Schneiderhan Marra N, Henzler T, Herget T, Joos T. Receptor tyrosine kinase inhibitor profiling using bead-based multiplex sandwich immunoassays. Methods Mol Biol. 2012;795:191-202 pubmed 出版商
  • 免疫沉淀; African green monkey
  • 免疫印迹; African green monkey
Yang X, Guo Z, Sun F, Li W, Alfano A, Shimelis H, et al. Novel membrane-associated androgen receptor splice variant potentiates proliferative and survival responses in prostate cancer cells. J Biol Chem. 2011;286:36152-60 pubmed 出版商
  • 免疫印迹; 人类
Deb T, Zuo A, Wang Y, Barndt R, Cheema A, Sengupta S, et al. Pnck induces ligand-independent EGFR degradation by probable perturbation of the Hsp90 chaperone complex. Am J Physiol Cell Physiol. 2011;300:C1139-54 pubmed 出版商
  • 免疫沉淀; 大鼠; 2 ug/ml
Poindexter N, Williams R, Powis G, Jen E, Caudle A, Chada S, et al. IL-24 is expressed during wound repair and inhibits TGFalpha-induced migration and proliferation of keratinocytes. Exp Dermatol. 2010;19:714-22 pubmed 出版商
  • 免疫印迹; 人类
Chou C, Jing N, Yamaguchi H, Tsou P, Lee H, Chen C, et al. High speed digital protein interaction analysis using microfluidic single molecule detection system. Lab Chip. 2010;10:1793-8 pubmed 出版商
  • 免疫细胞化学; 人类
Chung I, Akita R, Vandlen R, Toomre D, Schlessinger J, Mellman I. Spatial control of EGF receptor activation by reversible dimerization on living cells. Nature. 2010;464:783-7 pubmed 出版商
  • 免疫沉淀; 人类
  • 免疫细胞化学; 人类
Hsu S, Miller S, Wang Y, Hung M. Nuclear EGFR is required for cisplatin resistance and DNA repair. Am J Transl Res. 2009;1:249-58 pubmed
  • 免疫印迹; 人类
Zhang Y, Su H, Rahimi M, Tochihara R, Tang C. EGFRvIII-induced estrogen-independence, tamoxifen-resistance phenotype correlates with PgR expression and modulation of apoptotic molecules in breast cancer. Int J Cancer. 2009;125:2021-8 pubmed 出版商
  • 免疫沉淀; 人类; 图 6
  • 免疫细胞化学; 人类; 图 2
  • 免疫印迹; 人类; 图 6
Roepstorff K, Grandal M, Henriksen L, Knudsen S, Lerdrup M, Grøvdal L, et al. Differential effects of EGFR ligands on endocytic sorting of the receptor. Traffic. 2009;10:1115-27 pubmed 出版商
  • 免疫沉淀; 人类
Bitler B, Menzl I, Huerta C, Sands B, Knowlton W, Chang A, et al. Intracellular MUC1 peptides inhibit cancer progression. Clin Cancer Res. 2009;15:100-9 pubmed 出版商
  • 免疫印迹; 人类; 1:500
Klessner J, Desai B, Amargo E, Getsios S, Green K. EGFR and ADAMs cooperate to regulate shedding and endocytic trafficking of the desmosomal cadherin desmoglein 2. Mol Biol Cell. 2009;20:328-37 pubmed 出版商
  • 免疫细胞化学; 人类
Yoon H, Lee J, Randazzo P. ARAP1 regulates endocytosis of EGFR. Traffic. 2008;9:2236-52 pubmed 出版商
  • 免疫沉淀; 人类; 1:50
  • 免疫组化; 人类; 1:500
  • 免疫印迹; 人类; 1:500
Rajagopalan V, Zucker I, Jones J, Carlson M, MA Y. Cardiac ErbB-1/ErbB-2 mutant expression in young adult mice leads to cardiac dysfunction. Am J Physiol Heart Circ Physiol. 2008;295:H543-54 pubmed 出版商
  • 免疫沉淀; 人类
Umebayashi K, Stenmark H, Yoshimori T. Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation. Mol Biol Cell. 2008;19:3454-62 pubmed 出版商
  • 免疫沉淀; African green monkey
Xu W, Soga S, Beebe K, Lee M, Kim Y, Trepel J, et al. Sensitivity of epidermal growth factor receptor and ErbB2 exon 20 insertion mutants to Hsp90 inhibition. Br J Cancer. 2007;97:741-4 pubmed
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Yamasaki F, Zhang D, Bartholomeusz C, Sudo T, Hortobagyi G, Kurisu K, et al. Sensitivity of breast cancer cells to erlotinib depends on cyclin-dependent kinase 2 activity. Mol Cancer Ther. 2007;6:2168-77 pubmed
  • 免疫沉淀; 人类; 2 ug/time
Yamasaki F, Johansen M, Zhang D, Krishnamurthy S, Felix E, Bartholomeusz C, et al. Acquired resistance to erlotinib in A-431 epidermoid cancer cells requires down-regulation of MMAC1/PTEN and up-regulation of phosphorylated Akt. Cancer Res. 2007;67:5779-88 pubmed
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Faivre E, Lange C. Progesterone receptors upregulate Wnt-1 to induce epidermal growth factor receptor transactivation and c-Src-dependent sustained activation of Erk1/2 mitogen-activated protein kinase in breast cancer cells. Mol Cell Biol. 2007;27:466-80 pubmed
  • 免疫沉淀; 小鼠
Crotty T, Cai J, Sakane F, Taketomi A, Prescott S, Topham M. Diacylglycerol kinase delta regulates protein kinase C and epidermal growth factor receptor signaling. Proc Natl Acad Sci U S A. 2006;103:15485-90 pubmed
  • 免疫沉淀; 人类
Martin N, Mohney R, Dunn S, Das M, Scappini E, O Bryan J. Intersectin regulates epidermal growth factor receptor endocytosis, ubiquitylation, and signaling. Mol Pharmacol. 2006;70:1643-53 pubmed
  • 免疫细胞化学; 人类; 1:500
Funes M, Miller J, Lai C, Carraway K, Sweeney C. The mucin Muc4 potentiates neuregulin signaling by increasing the cell-surface populations of ErbB2 and ErbB3. J Biol Chem. 2006;281:19310-9 pubmed
  • 免疫沉淀; 人类
Lo H, Ali Seyed M, Wu Y, Bartholomeusz G, Hsu S, Hung M. Nuclear-cytoplasmic transport of EGFR involves receptor endocytosis, importin beta1 and CRM1. J Cell Biochem. 2006;98:1570-83 pubmed
  • 流式细胞仪; 人类; 10 ug/ml
Kim Y, Bhandari R, Cochran J, Kuriyan J, Wittrup K. Directed evolution of the epidermal growth factor receptor extracellular domain for expression in yeast. Proteins. 2006;62:1026-35 pubmed
  • 流式细胞仪; 小鼠; 10 ug/ml
Whitson K, Whitson S, Red Brewer M, McCoy A, Vitali A, Walker F, et al. Functional effects of glycosylation at Asn-579 of the epidermal growth factor receptor. Biochemistry. 2005;44:14920-31 pubmed
  • 免疫印迹; 人类
Pangburn H, Kraus H, Ahnen D, Rice P. Sulindac metabolites inhibit epidermal growth factor receptor activation and expression. J Carcinog. 2005;4:16 pubmed
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Xia W, Gerard C, Liu L, Baudson N, Ory T, Spector N. Combining lapatinib (GW572016), a small molecule inhibitor of ErbB1 and ErbB2 tyrosine kinases, with therapeutic anti-ErbB2 antibodies enhances apoptosis of ErbB2-overexpressing breast cancer cells. Oncogene. 2005;24:6213-21 pubmed
  • 免疫沉淀; African green monkey
Xu W, Yuan X, Xiang Z, Mimnaugh E, Marcu M, Neckers L. Surface charge and hydrophobicity determine ErbB2 binding to the Hsp90 chaperone complex. Nat Struct Mol Biol. 2005;12:120-6 pubmed
  • 免疫印迹; 人类; 图 7
Shen X, Kramer R. Adhesion-mediated squamous cell carcinoma survival through ligand-independent activation of epidermal growth factor receptor. Am J Pathol. 2004;165:1315-29 pubmed
Kaur H, Sparvoli D, Osakada H, Iwamoto M, Haraguchi T, Turkewitz A. An endosomal syntaxin and the AP-3 complex are required for formation and maturation of candidate lysosome-related secretory organelles (mucocysts) in Tetrahymena thermophila. Mol Biol Cell. 2017;28:1551-1564 pubmed 出版商
Pendharkar S, Mattoo S, Grover S. Sexual dysfunctions in alcohol-dependent men: A study from north India. Indian J Med Res. 2016;144:393-399 pubmed 出版商
Zelenko Z, Gallagher E, Tobin Hess A, Belardi V, Rostoker R, Blank J, et al. Silencing vimentin expression decreases pulmonary metastases in a pre-diabetic mouse model of mammary tumor progression. Oncogene. 2017;36:1394-1403 pubmed 出版商
Zhu H, Cao X, Ali Osman F, Keir S, Lo H. EGFR and EGFRvIII interact with PUMA to inhibit mitochondrial translocalization of PUMA and PUMA-mediated apoptosis independent of EGFR kinase activity. Cancer Lett. 2010;294:101-10 pubmed 出版商
Schmidt M, Hoeller D, Yu J, Furnari F, Cavenee W, Dikic I, et al. Alix/AIP1 antagonizes epidermal growth factor receptor downregulation by the Cbl-SETA/CIN85 complex. Mol Cell Biol. 2004;24:8981-93 pubmed
Fan Y, Wong L, Deb T, Johnson G. Ligand regulates epidermal growth factor receptor kinase specificity: activation increases preference for GAB1 and SHC versus autophosphorylation sites. J Biol Chem. 2004;279:38143-50 pubmed
Cochran J, Kim Y, Olsen M, Bhandari R, Wittrup K. Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments. J Immunol Methods. 2004;287:147-58 pubmed
Barzilay E, Ben Califa N, Supino Rosin L, Kashman Y, Hirschberg K, Elazar Z, et al. Geldanamycin-associated inhibition of intracellular trafficking is attributed to a co-purified activity. J Biol Chem. 2004;279:6847-52 pubmed
Nielsen U, Cardone M, Sinskey A, MacBeath G, Sorger P. Profiling receptor tyrosine kinase activation by using Ab microarrays. Proc Natl Acad Sci U S A. 2003;100:9330-5 pubmed
Schmidt M, Furnari F, Cavenee W, Bogler O. Epidermal growth factor receptor signaling intensity determines intracellular protein interactions, ubiquitination, and internalization. Proc Natl Acad Sci U S A. 2003;100:6505-10 pubmed
Cordero J, Cozzolino M, Lu Y, Vidal M, Slatopolsky E, Stahl P, et al. 1,25-Dihydroxyvitamin D down-regulates cell membrane growth- and nuclear growth-promoting signals by the epidermal growth factor receptor. J Biol Chem. 2002;277:38965-71 pubmed
Stoll S, Kansra S, Elder J. Metalloproteinases stimulate ErbB-dependent ERK signaling in human skin organ culture. J Biol Chem. 2002;277:26839-45 pubmed
Alper O -, Bergmann Leitner E, Bennett T, Hacker N, Stromberg K, Stetler Stevenson W. Epidermal growth factor receptor signaling and the invasive phenotype of ovarian carcinoma cells. J Natl Cancer Inst. 2001;93:1375-84 pubmed
Gaudry C, Palka H, Dusek R, Huen A, Khandekar M, Hudson L, et al. Tyrosine-phosphorylated plakoglobin is associated with desmogleins but not desmoplakin after epidermal growth factor receptor activation. J Biol Chem. 2001;276:24871-80 pubmed
Tikhomirov O, Carpenter G. Geldanamycin induces ErbB-2 degradation by proteolytic fragmentation. J Biol Chem. 2000;275:26625-31 pubmed
Tang C, Gong X, Moscatello D, Wong A, Lippman M. Epidermal growth factor receptor vIII enhances tumorigenicity in human breast cancer. Cancer Res. 2000;60:3081-7 pubmed
Supino Rosin L, Yoshimura A, Yarden Y, Elazar Z, Neumann D. Intracellular retention and degradation of the epidermal growth factor receptor, two distinct processes mediated by benzoquinone ansamycins. J Biol Chem. 2000;275:21850-5 pubmed
Supino Rosin L, Yoshimura A, Altaratz H, Neumann D. A cytosolic domain of the erythropoietin receptor contributes to endoplasmic reticulum-associated degradation. Eur J Biochem. 1999;263:410-9 pubmed
Baron A, Lafky J, Connolly D, Peoples J, O Kane D, Suman V, et al. A sandwich type acridinium-linked immunosorbent assay (ALISA) detects soluble ErbB1 (sErbB1) in normal human sera. J Immunol Methods. 1998;219:23-43 pubmed
图像
图像 1 :
赛默飞世尔 MA5-13319 图像 1
图像 2 :
赛默飞世尔 MA5-13319 图像 2
产品信息
产品类型 :
抗体
产品名称 :
表皮生长因子受体单克隆抗体(199.12)
目录# :
MA5-13319
规格 :
500微升
价格 :
美国425.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
人类
应用 :
Immunocytochemistry: 5 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 2-4 µg/mL
物种 :
人类
克隆 :
199.12
抗体亚型 :
IgG2a
储存 :
4° C
描述 :
EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
免疫原 :
细胞外区域人的重组表皮生长因子受体蛋白
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 5 µg/mL, Immunoprecipitation: 2 µg/mL, Western Blot: 2-4 µg/mL
别名 :
2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国