产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
表皮生长因子受体单克隆抗体(H9B4)
目录 :
MA5-13877
规格 :
500微升
价格 :
美国445.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
H9B4
反应物种 :
人类, 小鼠, fruit fly
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 25
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 1:1000; 图 4b
  • 免疫印迹; 人类; 1:1000; 图 4a
Wu Y, Wang Y, Lin Y, Liu Y, Wang Y, Jia J, et al. Dub3 inhibition suppresses breast cancer invasion and metastasis by promoting Snail1 degradation. Nat Commun. 2017;8:14228 pubmed 出版商
  • 免疫印迹; 人类; 图 1b
Day K, Lorenzatti Hiles G, Kozminsky M, Dawsey S, Paul A, Broses L, et al. HER2 and EGFR Overexpression Support Metastatic Progression of Prostate Cancer to Bone. Cancer Res. 2017;77:74-85 pubmed 出版商
  • 免疫组化; 人类; 1:200; 图 6a
  • 免疫印迹; 人类; 图 1b
Engel B, Bowser J, Broaddus R, Carson D. MUC1 stimulates EGFR expression and function in endometrial cancer. Oncotarget. 2016;7:32796-809 pubmed 出版商
  • 免疫沉淀; 人类
  • 免疫印迹; 人类
Arasada R, Amann J, Rahman M, Huppert S, Carbone D. EGFR blockade enriches for lung cancer stem-like cells through Notch3-dependent signaling. Cancer Res. 2014;74:5572-84 pubmed 出版商
  • 免疫印迹; 人类; 图 1
Grivas P, Day K, Karatsinides A, Paul A, Shakir N, Owainati I, et al. Evaluation of the antitumor activity of dacomitinib in models of human bladder cancer. Mol Med. 2013;19:367-76 pubmed 出版商
  • 免疫细胞化学; 人类
  • 免疫印迹; 人类
Odintsova E, van Niel G, Conjeaud H, Raposo G, Iwamoto R, Mekada E, et al. Metastasis suppressor tetraspanin CD82/KAI1 regulates ubiquitylation of epidermal growth factor receptor. J Biol Chem. 2013;288:26323-34 pubmed 出版商
  • 免疫沉淀; 小鼠; 1:1000
Brouxhon S, Kyrkanides S, Teng X, O Banion M, Clarke R, Byers S, et al. Soluble-E-cadherin activates HER and IAP family members in HER2+ and TNBC human breast cancers. Mol Carcinog. 2014;53:893-906 pubmed 出版商
  • 免疫印迹; 人类; 1:1000; 图 3
Neeraja Dharmaraj -, Engel B, Carson D. Activated EGFR stimulates MUC1 expression in human uterine and pancreatic cancer cell lines. J Cell Biochem. 2013;114:2314-22 pubmed 出版商
  • 免疫印迹; 人类; 1:5000; 图 1
Danysh B, Constantinou P, Lukianova Hleb E, Lapotko D, Carson D. The MUC1 Ectodomain: A Novel and Efficient Target for Gold Nanoparticle Clustering and Vapor Nanobubble Generation. Theranostics. 2012;2:777-87 pubmed 出版商
  • 免疫印迹; 人类
Fan P, McDaniel R, Kim H, Clagett D, Haddad B, Jordan V. Modulating therapeutic effects of the c-Src inhibitor via oestrogen receptor and human epidermal growth factor receptor 2 in breast cancer cell lines. Eur J Cancer. 2012;48:3488-98 pubmed 出版商
  • 免疫沉淀; 人类; 1 ug/time
  • 免疫印迹; 人类
Grabowska M, Sandhu B, Day M. EGF promotes the shedding of soluble E-cadherin in an ADAM10-dependent manner in prostate epithelial cells. Cell Signal. 2012;24:532-8 pubmed 出版商
  • 免疫沉淀; 人类; 2-5 ug/time
DeFazio Eli L, Strommen K, Dao Pick T, Parry G, Goodman L, Winslow J. Quantitative assays for the measurement of HER1-HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action. Breast Cancer Res. 2011;13:R44 pubmed 出版商
  • 免疫细胞化学; 人类
Shi Y, Huang W, Tan Y, Jin X, Dua R, Penuel E, et al. A novel proximity assay for the detection of proteins and protein complexes: quantitation of HER1 and HER2 total protein expression and homodimerization in formalin-fixed, paraffin-embedded cell lines and breast cancer tissue. Diagn Mol Pathol. 2009;18:11-21 pubmed 出版商
  • 免疫印迹; 人类
Deb T, Coticchia C, Barndt R, Zuo H, Dickson R, Johnson M. Pregnancy-upregulated nonubiquitous calmodulin kinase induces ligand-independent EGFR degradation. Am J Physiol Cell Physiol. 2008;295:C365-77 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:50; 图 5
  • 免疫印迹; 小鼠; 图 3a
Choi S, Mendrola J, Lemmon M. EGF-independent activation of cell-surface EGF receptors harboring mutations found in gefitinib-sensitive lung cancer. Oncogene. 2007;26:1567-76 pubmed
  • 免疫印迹; 人类; 1:1000
Blehm K, Spiess P, Bondaruk J, Dujka M, Villares G, Zhao Y, et al. Mutations within the kinase domain and truncations of the epidermal growth factor receptor are rare events in bladder cancer: implications for therapy. Clin Cancer Res. 2006;12:4671-7 pubmed
  • 免疫印迹; 人类
Odintsova E, Butters T, Monti E, Sprong H, van Meer G, Berditchevski F. Gangliosides play an important role in the organization of CD82-enriched microdomains. Biochem J. 2006;400:315-25 pubmed
  • 免疫印迹; 人类
Contessa J, Abell A, Valerie K, Lin P, Schmidt Ullrich R. ErbB receptor tyrosine kinase network inhibition radiosensitizes carcinoma cells. Int J Radiat Oncol Biol Phys. 2006;65:851-8 pubmed
  • 免疫印迹; 人类; 1:1000; 图 6
Kraemer K, Schmidt U, Fuessel S, Herr A, Wirth M, Meye A. Microarray analyses in bladder cancer cells: inhibition of hTERT expression down-regulates EGFR. Int J Cancer. 2006;119:1276-84 pubmed
  • 免疫沉淀; 人类
Contessa J, Abell A, Mikkelsen R, Valerie K, Schmidt Ullrich R. Compensatory ErbB3/c-Src signaling enhances carcinoma cell survival to ionizing radiation. Breast Cancer Res Treat. 2006;95:17-27 pubmed
  • 免疫印迹; fruit fly ; 图 4b
Dawson J, Berger M, Lin C, Schlessinger J, Lemmon M, Ferguson K. Epidermal growth factor receptor dimerization and activation require ligand-induced conformational changes in the dimer interface. Mol Cell Biol. 2005;25:7734-42 pubmed
  • 免疫沉淀; 人类
Sturla L, Amorino G, Alexander M, Mikkelsen R, Valerie K, Schmidt Ullrichr R. Requirement of Tyr-992 and Tyr-1173 in phosphorylation of the epidermal growth factor receptor by ionizing radiation and modulation by SHP2. J Biol Chem. 2005;280:14597-604 pubmed
  • 免疫印迹; 人类
Kansra S, Stoll S, Johnson J, Elder J. Src family kinase inhibitors block amphiregulin-mediated autocrine ErbB signaling in normal human keratinocytes. Mol Pharmacol. 2005;67:1145-57 pubmed
Tamura S, Wang Y, Veeneman B, Hovelson D, Bankhead A, Broses L, et al. Molecular Correlates of In Vitro Responses to Dacomitinib and Afatinib in Bladder Cancer. Bladder Cancer. 2018;4:77-90 pubmed 出版商
Genther S, Sterling S, Duensing S, Munger K, Sattler C, Lambert P. Quantitative role of the human papillomavirus type 16 E5 gene during the productive stage of the viral life cycle. J Virol. 2003;77:2832-42 pubmed
图像
图像 1 :
赛默飞世尔 MA5-13877 图像 1
图像 2 :
赛默飞世尔 MA5-13877 图像 2
图像 3 :
赛默飞世尔 MA5-13877 图像 3
产品信息
产品类型 :
抗体
产品名称 :
表皮生长因子受体单克隆抗体(H9B4)
目录# :
MA5-13877
规格 :
500微升
价格 :
美国445.00
克隆性 :
单克隆
纯度 :
蛋白质G
宿主 :
小鼠
反应物种 :
人类
应用 :
Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL
物种 :
人类
克隆 :
H9B4
抗体亚型 :
IgG1
储存 :
4° C
描述 :
EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
免疫原 :
纯化表皮生长因子受体来自A431细胞
格式 :
液体
应用w/稀释 :
Immunoprecipitation: 2 µg/mL, Western Blot: 1-2 µg/mL
别名 :
2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国