产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
p53单克隆抗体(SP5)
目录 :
MA5-14516
规格 :
500微升
价格 :
美国446.00
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
SP5
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 26
出版应用/物种/样本/稀释参考文献
  • 免疫组化-石蜡切片; 人类; 1:100; 表 1
Chung M, Lee J, Kim S, Suh Y, Choi H. Simple Prediction Model of Axillary Lymph Node Positivity After Analyzing Molecular and Clinical Factors in Early Breast Cancer. Medicine (Baltimore). 2016;95:e3689 pubmed 出版商
  • 免疫组化; 人类; 1:200
Suryavanshi M, Mehta A, Jaipuria J, Sharma A, Rawal S, Seth N. Weaker ERG expression in patients with ERG-positive prostate cancer is associated with advanced disease and weaker androgen receptor expression: An Indian outlook. Urol Oncol. 2015;33:331.e9-15 pubmed 出版商
  • 免疫组化; 人类; 1:200
Khandakar B, Mathur S, Kumar L, Kumar S, Datta Gupta S, Iyer V, et al. Tissue biomarkers in prognostication of serous ovarian cancer following neoadjuvant chemotherapy. Biomed Res Int. 2014;2014:401245 pubmed 出版商
  • 免疫组化; 人类; 1:100
Staff S, Tolonen T, Laasanen S, Mecklin J, Isola J, Maenpaa J. Quantitative analysis of γ-H2AX and p53 nuclear expression levels in ovarian and fallopian tube epithelium from risk-reducing salpingo-oophorectomies in BRCA1 and BRCA2 mutation carriers. Int J Gynecol Pathol. 2014;33:309-16 pubmed 出版商
  • 免疫组化; 人类
Ozer H, Yenicesu G, Arici S, Cetin M, Tuncer E, Cetin A. Immunohistochemistry with apoptotic-antiapoptotic proteins (p53, p21, bax, bcl-2), c-kit, telomerase, and metallothionein as a diagnostic aid in benign, borderline, and malignant serous and mucinous ovarian tumors. Diagn Pathol. 2012;7:124 pubmed 出版商
  • 免疫组化; 人类; 1:1000
Meijer D, de Jong D, Pansuriya T, van den Akker B, Picci P, Szuhai K, et al. Genetic characterization of mesenchymal, clear cell, and dedifferentiated chondrosarcoma. Genes Chromosomes Cancer. 2012;51:899-909 pubmed 出版商
  • 免疫组化; 人类
Meijer Jorna L, van der Loos C, Teeling P, de Boer O, Florquin S, van der Horst C, et al. Proliferation and maturation of microvessels in arteriovenous malformations--expression patterns of angiogenic and cell cycle-dependent factors. J Cutan Pathol. 2012;39:610-20 pubmed 出版商
  • 免疫组化; 人类; 1:50
Perisanidis C, Perisanidis B, Wrba F, Brandstetter A, El Gazzar S, Papadogeorgakis N, et al. Evaluation of immunohistochemical expression of p53, p21, p27, cyclin D1, and Ki67 in oral and oropharyngeal squamous cell carcinoma. J Oral Pathol Med. 2012;41:40-6 pubmed 出版商
  • 免疫组化; 人类; 1:50
Schuster C, Berger A, Hoelzl M, Putz E, Frenzel A, Simma O, et al. The cooperating mutation or "second hit" determines the immunologic visibility toward MYC-induced murine lymphomas. Blood. 2011;118:4635-45 pubmed 出版商
  • 免疫组化; 人类; 1:100
Lee D, Kim S, Suh Y, Kim S, Kim H, Shim B. Clinical implication of p53 overexpression in breast cancer patients younger than 50 years with a triple-negative subtype who undergo a modified radical mastectomy. Jpn J Clin Oncol. 2011;41:854-66 pubmed 出版商
  • 免疫组化; 人类; 1:500
Sciuscio D, Diserens A, van Dommelen K, Martinet D, Jones G, Janzer R, et al. Extent and patterns of MGMT promoter methylation in glioblastoma- and respective glioblastoma-derived spheres. Clin Cancer Res. 2011;17:255-66 pubmed 出版商
  • 免疫组化; 人类; 1:1
Abdelzaher E, El Gendi S, Yehya A, Gowil A. Recurrence of benign meningiomas: predictive value of proliferative index, BCL2, p53, hormonal receptors and HER2 expression. Br J Neurosurg. 2011;25:707-13 pubmed 出版商
  • 免疫组化; 人类
Krishnamurti U, Zarineh A, Atem F, Silverman J. Correlation of immunohistochemical expression of p53 with unamplified chromosome 17 polysomy in invasive breast carcinoma. Appl Immunohistochem Mol Morphol. 2011;19:28-32 pubmed 出版商
  • 免疫组化; 人类; 1:100
Myong N. Role of loss of o?-methylguanine dna methyltransferase (MGMT) expression in non-small cell lung carcinomas (NSCLCs): with reference to the relationship with p53 overexpression. Cancer Res Treat. 2010;42:95-100 pubmed 出版商
  • 免疫组化; 人类
Bozkurt S, Ayan E, Bolukbasi F, Elmaci I, Pamir N, Sav A. Immunohistochemical expression of SPARC is correlated with recurrence, survival and malignant potential in meningiomas. APMIS. 2009;117:651-9 pubmed 出版商
  • 免疫组化; 人类; 1:40
Wang R, Wen M, Wang J, Ho S, Jan Y. Osteosarcoma arising in a long-standing uterine leiomyoma: a case report and literature review. Int J Surg Pathol. 2011;19:99-103 pubmed 出版商
  • 免疫组化; 大鼠
Arikan Y, Tosun M, Yilmaz S, Saykol V, Söylemez Z. The comparative effects of pneumoperitoneum on apoptosis and p53 expression in gastrointestinal organs. J Laparoendosc Adv Surg Tech A. 2008;18:365-71 pubmed 出版商
  • 免疫组化; 人类; 1:25
Song Z, Wang Y, Xie L, Zang X, Yin H. Expression of senescence-related genes in human corneal endothelial cells. Mol Vis. 2008;14:161-70 pubmed
  • 免疫组化; 人类; 200 ug/ml
Aktas S, Kargi A, Olgun N, Diniz G, Erbay A, Vergin C. Prognostic significance of cell proliferation and apoptosis-regulating proteins in Epstein-Barr virus positive and negative pediatric Hodgkin lymphoma. Lymphat Res Biol. 2007;5:175-82 pubmed
  • 免疫组化; 小鼠
Navas T, Nguyen A, Hideshima T, Reddy M, Ma J, Haghnazari E, et al. Inhibition of p38alpha MAPK enhances proteasome inhibitor-induced apoptosis of myeloma cells by modulating Hsp27, Bcl-X(L), Mcl-1 and p53 levels in vitro and inhibits tumor growth in vivo. Leukemia. 2006;20:1017-27 pubmed
Xia W, Bai H, Deng Y, Yang Y. PLA2G16 is a mutant p53/KLF5 transcriptional target and promotes glycolysis of pancreatic cancer. J Cell Mol Med. 2020;24:12642-12655 pubmed 出版商
Lee K, Piett C, Andrews J, Mann E, Nagel Z, Gassman N. Defective base excision repair in the response to DNA damaging agents in triple negative breast cancer. PLoS ONE. 2019;14:e0223725 pubmed 出版商
Casadei L, Calore F, Braggio D, Zewdu A, Deshmukh A, Fadda P, et al. MDM2 Derived from Dedifferentiated Liposarcoma Extracellular Vesicles Induces MMP2 Production from Preadipocytes. Cancer Res. 2019;79:4911-4922 pubmed 出版商
Salmina K, Huna A, Inashkina I, Belyayev A, Krigerts J, Paštová L, et al. Nucleolar aggresomes mediate release of pericentric heterochromatin and nuclear destruction of genotoxically treated cancer cells. Nucleus. 2017;8:205-221 pubmed 出版商
Verreault M, Schmitt C, Goldwirt L, Pelton K, Haidar S, Levasseur C, et al. Preclinical Efficacy of the MDM2 Inhibitor RG7112 in MDM2-Amplified and TP53 Wild-type Glioblastomas. Clin Cancer Res. 2016;22:1185-96 pubmed 出版商
Lee D, Kim S, Kim S, Suh Y, Kim H, Shim B. Prognostic significance of breast cancer subtype and p53 overexpression in patients with locally advanced or high-risk breast cancer treated using upfront modified radical mastectomy with or without post-mastectomy radiation therapy. Int J Clin Oncol. 2012;17:447-55 pubmed 出版商
图像
图像 1 :
赛默飞世尔 MA5-14516 图像 1
Formalin-fixed, paraffin-embedded human colon carcinoma stained with p53 using peroxidase-conjugate and AEC. Note nuclear staining of tumor cells.
图像 2 :
赛默飞世尔 MA5-14516 图像 2
Immunofluorescence staining of p21 and p53 in A549 cells treated with media only (non-treated) or with 5 µM camptothecin for 20 hours.
图像 3 :
赛默飞世尔 MA5-14516 图像 3
Immunofluorescence staining of p21 and p53 in A549 cells treated with media only (non-treated) or with 5 µM camptothecin for 20 hours.
产品信息
产品类型 :
抗体
产品名称 :
p53单克隆抗体(SP5)
目录# :
MA5-14516
规格 :
500微升
价格 :
美国446.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
兔
反应物种 :
人类
应用 :
Flow Cytometry: 1:200, Immunocytochemistry: 1:25, Immunohistochemistry (Paraffin): 1:100, Western Blot: 1:10-1:500
物种 :
人类
克隆 :
SP5
抗体亚型 :
IgG
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.
免疫原 :
Recombinant human full-length wild type p53 protein
格式 :
液体
应用w/稀释 :
Flow Cytometry: 1:200, Immunocytochemistry: 1:25, Immunohistochemistry (Paraffin): 1:100, Western Blot: 1:10-1:500
别名 :
Antigen NY-CO-13; bbl; BCC7; bfy; bhy; cellular tumor antigen p53; Cys 51 Stop; EGK_08142; FLJ92943; HGNC11998; I79_002739; LFS1; Li-Fraumeni syndrome; mutant p53; mutant tumor protein 53; OTTMUSP00000006194; p44; p53; p53 cellular tumor antigen; p53 protein; p53 tumor suppressor; p53 tumor suppressor phosphoprotein; phosphoprotein p53; Tp53; tp53.L; transformation related protein 53; transformation-related protein 53; Trp248; Trp53; tumor protein 53; tumor protein p53; tumor protein p53 (Li-Fraumeni syndrome); tumor protein p53 L homeolog; tumor suppressor p53; tumor suppressor p53 phosphoprotein; tumor suppressor protein p53; tumor supressor p53; Tumour Protein p53; XELAEV_180196761mg; Xp53; Xrel3
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国