产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
绿色荧光蛋白单克隆抗体(GF28R), HRP
目录 :
MA5-15256-HRP
规格 :
50微升
价格 :
美国236.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
HRP
克隆名称 :
GF28R
应用 :
免疫印迹
更多信息或购买 :
文章摘录数: 9
| 参考文献 |
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Anderson D, Makarewich C, Anderson K, Shelton J, Bezprozvannaya S, Bassel Duby R, et al. Widespread control of calcium signaling by a family of SERCA-inhibiting micropeptides. Sci Signal. 2016;9:ra119 pubmed
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图像
图像 1 :
Western blot analysis of GFP was performed by loading various amounts of recombinant GFP per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated GFP monoclonal antibody (Product # MA5-15256-HRP) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
产品信息
产品类型 :
抗体
产品名称 :
绿色荧光蛋白单克隆抗体(GF28R), HRP
目录# :
MA5-15256-HRP
规格 :
50微升
价格 :
美国236.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
标签
应用 :
Western Blot: 1:500-1:2,000
物种 :
标签
克隆 :
GF28R
抗体亚型 :
IgG1
储存 :
4° C
描述 :
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
免疫原 :
GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugated.
格式 :
液体
应用w/稀释 :
Western Blot: 1:500-1:2,000
别名 :
GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
更多信息或购买 :
公司信息
赛默飞世尔