产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
GAPDH Loading Control Monoclonal Antibody (GA1R), HRP
目录 :
MA5-15738-HRP
规格 :
50µL
价格 :
225.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
HRP
克隆名称 :
GA1R
反应物种 :
中国人仓鼠, 人类, 小鼠, 大鼠, , domestic rabbit
应用 :
免疫印迹
更多信息或购买 :
文章摘录数: 21
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类
Liu Y, Chen C, Xu Z, Scuoppo C, Rillahan C, Gao J, et al. Deletions linked to TP53 loss drive cancer through p53-independent mechanisms. Nature. 2016;531:471-475 pubmed 出版商
Dimas R, Jordan B, Jiang X, Martini C, Glavy J, Patterson D, et al. Engineering DNA recognition and allosteric response properties of TetR family proteins by using a module-swapping strategy. Nucleic Acids Res. 2019;47:8913-8925 pubmed 出版商
Meydan S, Marks J, Klepacki D, Sharma V, Baranov P, Firth A, et al. Retapamulin-Assisted Ribosome Profiling Reveals the Alternative Bacterial Proteome. Mol Cell. 2019;74:481-493.e6 pubmed 出版商
Zhang J, Termglinchan V, Shao N, Itzhaki I, Liu C, Ma N, et al. A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles. Cell Stem Cell. 2019;24:802-811.e5 pubmed 出版商
Aho E, Wang J, Gogliotti R, Howard G, Phan J, Acharya P, et al. Displacement of WDR5 from Chromatin by a WIN Site Inhibitor with Picomolar Affinity. Cell Rep. 2019;26:2916-2928.e13 pubmed 出版商
Demirgan S, Akyol O, Temel Z, Şengelen A, Pekmez M, Demirgan R, et al. Isoflurane exposure in infant rats acutely increases aquaporin 4 and does not cause neurocognitive impairment. Bosn J Basic Med Sci. 2019;: pubmed 出版商
Cops J, Mullens W, Verbrugge F, Swennen Q, De Moor B, Reynders C, et al. Selective abdominal venous congestion induces adverse renal and hepatic morphological and functional alterations despite a preserved cardiac function. Sci Rep. 2018;8:17757 pubmed 出版商
Ibrahim S, Xie W, Strong J, Tonello R, Berta T, Zhang J. Mineralocorticoid Antagonist Improves Glucocorticoid Receptor Signaling and Dexamethasone Analgesia in an Animal Model of Low Back Pain. Front Cell Neurosci. 2018;12:453 pubmed 出版商
Villiger L, Grisch Chan H, Lindsay H, Ringnalda F, Pogliano C, Allegri G, et al. Treatment of a metabolic liver disease by in vivo genome base editing in adult mice. Nat Med. 2018;24:1519-1525 pubmed 出版商
Bradley T, Peppa D, Pedroza Pacheco I, Li D, Cain D, Henao R, et al. RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses. Cell. 2018;175:387-399.e17 pubmed 出版商
Nguyen T, Duchon A, Manousopoulou A, Loaëc N, Villiers B, Pani G, et al. Correction of cognitive deficits in mouse models of Down syndrome by a pharmacological inhibitor of DYRK1A. Dis Model Mech. 2018;11: pubmed 出版商
Yu J, Li Y, Wang T, Zhong X. Modification of N6-methyladenosine RNA methylation on heat shock protein expression. PLoS ONE. 2018;13:e0198604 pubmed 出版商
Halperin Kuhns V, Pluznick J. Novel differences in renal gene expression in a diet-induced obesity model. Am J Physiol Renal Physiol. 2018;314:F517-F530 pubmed 出版商
Wierzbicki M, Jaworski S, Kutwin M, Grodzik M, Strojny B, Kurantowicz N, et al. Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion. Int J Nanomedicine. 2017;12:7241-7254 pubmed 出版商
Xu Y, Wang Y, Yao A, Xu Z, Dou H, Shen S, et al. Low Frequency Magnetic Fields Induce Autophagy-associated Cell Death in Lung Cancer through miR-486-mediated Inhibition of Akt/mTOR Signaling Pathway. Sci Rep. 2017;7:11776 pubmed 出版商
Nardone G, Oliver De La Cruz J, Vrbsky J, Martini C, Pribyl J, Skladal P, et al. YAP regulates cell mechanics by controlling focal adhesion assembly. Nat Commun. 2017;8:15321 pubmed 出版商
Wild F, Khan M, Straka T, Rudolf R. Progress of endocytic CHRN to autophagic degradation is regulated by RAB5-GTPase and T145 phosphorylation of SH3GLB1 at mouse neuromuscular junctions in vivo. Autophagy. 2016;12:2300-2310 pubmed
Charrier A, Wang L, Stephenson E, Ghanta S, Ko C, Croniger C, et al. Zinc finger protein 407 overexpression upregulates PPAR target gene expression and improves glucose homeostasis in mice. Am J Physiol Endocrinol Metab. 2016;311:E869-E880 pubmed 出版商
Ortiz D, Glassbrook J, Pellett P. Protein-Protein Interactions Suggest Novel Activities of Human Cytomegalovirus Tegument Protein pUL103. J Virol. 2016;90:7798-810 pubmed 出版商
Zhang L, Tran N, Su H, Wang R, Lu Y, Tang H, et al. Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing. elife. 2015;4: pubmed 出版商
Montoya V, Fan H, Bryar P, Weinstein J, Mets M, Feng G, et al. Novel miRNA-31 and miRNA-200a-Mediated Regulation of Retinoblastoma Proliferation. PLoS ONE. 2015;10:e0138366 pubmed 出版商
图像
图像 1 :
赛默飞世尔 MA5-15738-HRP 图像 1
Western blot analysis of GAPDH was performed by loading 50ug of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated GAPDH monoclonal antibody (Product # MA5-15738-HRP) at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
产品信息
产品类型 :
抗体
产品名称 :
GAPDH Loading Control Monoclonal Antibody (GA1R), HRP
目录# :
MA5-15738-HRP
规格 :
50µL
价格(美元) :
225.00美元
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
细菌, 鸡, 仓鼠, 人类, 昆虫, 小鼠, 兔, 大鼠, 酵母
应用 :
Western Blot: 1:500-1:2000
物种 :
细菌, 鸡, 仓鼠, 人类, 昆虫, 小鼠, 兔, 大鼠, 酵母
克隆 :
GA1R
抗体亚型 :
IgG1
储存 :
4° C
描述 :
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
免疫原 :
重组磷酸甘油醛脱氢酶
格式 :
液体
应用w/稀释 :
Western Blot: 1:500-1:2000
别名 :
38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; bb02e05; cb350; cb609; CDABP0047; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; fb71f08; fk58c09; G3PD; G3PDH; Gapd; GAPD2; gapdh; GAPDH2; GAPDH-2; GAPDHS; GAPDS; Gapd-s; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; glyceraldehyde phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase GAPDH; glyceraldehyde-3-phosphate dehydrogenase like-17 protein; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; glyceraldehyde-phosphate-dehydrogenase; glycerine aldehyde 3-phosphate dehydrogenase; HEL-S-162eP; HEL-S-278; HGNC:4141; HSD35; HSD-35; I79_001391; KNC-NDS6; LOW QUALITY PROTEIN: glyceraldehyde-3-phosphate dehydrogenase, testis-specific; mg:bb02e05; MGC128279 protein; MGC88685; OK/SW-cl.12; Peptidyl-cysteine S-nitrosylase GAPDH; similar to glyceraldehyde 3-phosphate dehydrogenase; spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; Unknown (protein for IMAGE:8101613); wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国