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NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
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GAPDH Loading Control Monoclonal Antibody (GA1R)
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酵母菌目, fission yeast, 中国人仓鼠, 幽门螺杆菌, 人类, 小鼠, 大鼠, , , pigs , brewer's yeast, domestic rabbit, roundworm
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片
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文章摘录数: 297
  • 免疫印迹; 人类; 图 9
Wenta T, Rychlowski M, Jarzab M, Lipinska B. HtrA4 Protease Promotes Chemotherapeutic-Dependent Cancer Cell Death. Cells. 2019;8: pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:1000; 图 4c
Wyżewski Z, Gregorczyk Zboroch K, Mielcarska M, Bossowska Nowicka M, Struzik J, Szczepanowska J, et al. Mitochondrial Heat Shock Response Induced by Ectromelia Virus is Accompanied by Reduced Apoptotic Potential in Murine L929 Fibroblasts. Arch Immunol Ther Exp (Warsz). 2019;67:401-414 pubmed 出版商
  • 免疫印迹; 人类; 1:1000
Chung H, Zou X, Bajar B, Brand V, Huo Y, Alcudia J, et al. A compact synthetic pathway rewires cancer signaling to therapeutic effector release. Science. 2019;364: pubmed 出版商
  • 免疫印迹; 人类; 图 3d
Leoz M, Kukanja P, Luo Z, Huang F, Cary D, Peterlin B, et al. HEXIM1-Tat chimera inhibits HIV-1 replication. PLoS Pathog. 2018;14:e1007402 pubmed 出版商
  • 免疫印迹; 小鼠; 1:5000; 图 9a
Whitson J, Zhang X, Medvedovic M, Chen J, Wei Z, Monnier V, et al. Transcriptome of the GSH-Depleted Lens Reveals Changes in Detoxification and EMT Signaling Genes, Transport Systems, and Lipid Homeostasis. Invest Ophthalmol Vis Sci. 2017;58:2666-2684 pubmed 出版商
  • 免疫印迹; 小鼠; 图 s1b
Bi P, Ramirez Martinez A, Li H, Cannavino J, McAnally J, Shelton J, et al. Control of muscle formation by the fusogenic micropeptide myomixer. Science. 2017;356:323-327 pubmed 出版商
  • 免疫印迹; 人类; 图 4e
AlAmri M, Kadri H, Alderwick L, Simpkins N, Mehellou Y. Rafoxanide and Closantel Inhibit SPAK and OSR1 Kinases by Binding to a Highly Conserved Allosteric Site on Their C-terminal Domains. ChemMedChem. 2017;12:639-645 pubmed 出版商
  • 免疫印迹; brewer's yeast; 图 1c
Suresh S, Chavalmane A, Dj V, Yarreiphang H, Rai S, Paul A, et al. A novel autophagy modulator 6-Bio ameliorates SNCA/?-synuclein toxicity. Autophagy. 2017;13:1221-1234 pubmed 出版商
  • 免疫印迹; 小鼠; 1:10,000; 图 4f
Xiang J, Yang S, Xin N, Gaertig M, Reeves R, Li S, et al. DYRK1A regulates Hap1-Dcaf7/WDR68 binding with implication for delayed growth in Down syndrome. Proc Natl Acad Sci U S A. 2017;114:E1224-E1233 pubmed 出版商
  • 免疫印迹; 人类; 1:4000; 图 1a
Guo R, Si R, Scott B, Makino A. Mitochondrial connexin40 regulates mitochondrial calcium uptake in coronary endothelial cells. Am J Physiol Cell Physiol. 2017;312:C398-C406 pubmed 出版商
  • 免疫印迹; 人类
Miroshnychenko O, Chang W, Dragoo J. The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration. Am J Sports Med. 2017;45:945-953 pubmed 出版商
  • 免疫印迹; 小鼠; 1:2000; 图 1c
Zhang D, Wu B, Wang P, Wang Y, Lu P, Nechiporuk T, et al. Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts. Nucleic Acids Res. 2017;45:3102-3115 pubmed 出版商
  • 免疫印迹; 小鼠
Radhakrishnan V, Gilpatrick M, Parsa N, Kiela P, Ghishan F. Expression of Cav1.3 calcium channel in the human and mouse colon: posttranscriptional inhibition by IFN?. Am J Physiol Gastrointest Liver Physiol. 2017;312:G77-G84 pubmed 出版商
  • 免疫印迹; 人类; 图 2b
Hwang D, Jo H, Hwang S, Kim J, Kim I, Lim Y. Conditioned medium from LS 174T goblet cells treated with oxyresveratrol strengthens tight junctions in Caco-2 cells. Biomed Pharmacother. 2017;85:280-286 pubmed 出版商
  • 免疫印迹; 小鼠; 图 5d
Liu L, Tao Z, Zheng L, Brooke J, Smith C, Liu D, et al. FoxO1 interacts with transcription factor EB and differentially regulates mitochondrial uncoupling proteins via autophagy in adipocytes. Cell Death Discov. 2016;2:16066 pubmed
  • 免疫印迹; 人类; 图 4b
Nguyen A, Nyberg K, Scott M, Welsh A, Nguyen A, Wu N, et al. Stiffness of pancreatic cancer cells is associated with increased invasive potential. Integr Biol (Camb). 2016;8:1232-1245 pubmed
  • 免疫印迹; 小鼠; 图 5a
Charrier A, Wang L, Stephenson E, Ghanta S, Ko C, Croniger C, et al. Zinc finger protein 407 overexpression upregulates PPAR target gene expression and improves glucose homeostasis in mice. Am J Physiol Endocrinol Metab. 2016;311:E869-E880 pubmed 出版商
  • 免疫印迹; 人类; 图 1b
Wang Y, Lin S, Hsieh P, Hung S. Concomitant beige adipocyte differentiation upon induction of mesenchymal stem cells into brown adipocytes. Biochem Biophys Res Commun. 2016;478:689-95 pubmed 出版商
  • 免疫印迹; fission yeast; 图 3
Al Sady B, Greenstein R, El Samad H, Braun S, Madhani H. Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics. PLoS ONE. 2016;11:e0159292 pubmed 出版商
  • 免疫印迹; 人类; 1:1500; 图 6
Edmondson R, Adcock A, Yang L. Influence of Matrices on 3D-Cultured Prostate Cancer Cells' Drug Response and Expression of Drug-Action Associated Proteins. PLoS ONE. 2016;11:e0158116 pubmed 出版商
  • 免疫印迹; 人类; 图 6
Justis A, Hansen B, Beare P, King K, Heinzen R, Gilk S. Interactions between the Coxiella burnetii parasitophorous vacuole and the endoplasmic reticulum involve the host protein ORP1L. Cell Microbiol. 2017;19: pubmed 出版商
  • 免疫印迹; 人类; 图 3
Ortiz D, Glassbrook J, Pellett P. Protein-Protein Interactions Suggest Novel Activities of Human Cytomegalovirus Tegument Protein pUL103. J Virol. 2016;90:7798-810 pubmed 出版商
  • 免疫印迹; 小鼠; 图 2
Ryan T, Schmidt C, Green T, Spangenburg E, Neufer P, McClung J. Targeted Expression of Catalase to Mitochondria Protects Against Ischemic Myopathy in High-Fat Diet-Fed Mice. Diabetes. 2016;65:2553-68 pubmed 出版商
  • 免疫印迹; 小鼠; 图 1
Liu L, Zheng L, Zou P, Brooke J, Smith C, Long Y, et al. FoxO1 antagonist suppresses autophagy and lipid droplet growth in adipocytes. Cell Cycle. 2016;15:2033-41 pubmed 出版商
  • 免疫印迹; 小鼠; 1:2000; 图 1c
Høydal M, Stølen T, Kettlewell S, Maier L, Brown J, Sowa T, et al. Exercise training reverses myocardial dysfunction induced by CaMKII?C overexpression by restoring Ca2+ homeostasis. J Appl Physiol (1985). 2016;121:212-20 pubmed 出版商
  • 免疫印迹; roundworm ; 1:5000; 图 2
Lee J, Kwon G, Park J, Kim J, Lim Y. Brief Communication: SIR-2.1-dependent lifespan extension of Caenorhabditis elegans by oxyresveratrol and resveratrol. Exp Biol Med (Maywood). 2016;241:1757-63 pubmed 出版商
  • 免疫印迹; 人类; 图 4
Tran N, Su H, Khodadadi Jamayran A, Lin S, Zhang L, Zhou D, et al. The AS-RBM15 lncRNA enhances RBM15 protein translation during megakaryocyte differentiation. EMBO Rep. 2016;17:887-900 pubmed 出版商
  • 免疫印迹; 人类; 图 1a
Nguyen J, Bernert R, In K, Kang P, Sebastiao N, Hu C, et al. Gamma-interferon-inducible lysosomal thiol reductase is upregulated in human melanoma. Melanoma Res. 2016;26:125-37 pubmed 出版商
  • 免疫印迹; 人类
Haven B, Heilig E, Donham C, Settles M, Vasilevsky N, Owen K. Registered report: A chromatin-mediated reversible drug-tolerant state in cancer cell subpopulations. elife. 2016;5: pubmed 出版商
  • 免疫印迹; 人类; 1:1000; 图 1a
Li Y, Banerjee S, Wang Y, Goldstein S, Dong B, Gaughan C, et al. Activation of RNase L is dependent on OAS3 expression during infection with diverse human viruses. Proc Natl Acad Sci U S A. 2016;113:2241-6 pubmed 出版商
  • 免疫印迹; 人类; 1:5000; 图 2
Weilner S, Keider V, Winter M, Harreither E, Salzer B, Weiss F, et al. Vesicular Galectin-3 levels decrease with donor age and contribute to the reduced osteo-inductive potential of human plasma derived extracellular vesicles. Aging (Albany NY). 2016;8:16-33 pubmed
  • 免疫印迹; 人类; 图 1
Zhang L, Tran N, Su H, Wang R, Lu Y, Tang H, et al. Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing. elife. 2015;4: pubmed 出版商
  • 免疫印迹; 小鼠; 图 6
Rolyan H, Tyurina Y, Hernandez M, Amoscato A, Sparvero L, Nmezi B, et al. Defects of Lipid Synthesis Are Linked to the Age-Dependent Demyelination Caused by Lamin B1 Overexpression. J Neurosci. 2015;35:12002-17 pubmed 出版商
  • 免疫印迹; 小鼠; 1:100; 图 2d
Volta M, Cataldi S, Beccano Kelly D, Munsie L, Tatarnikov I, Chou P, et al. Chronic and acute LRRK2 silencing has no long-term behavioral effects, whereas wild-type and mutant LRRK2 overexpression induce motor and cognitive deficits and altered regulation of dopamine release. Parkinsonism Relat Disord. 2015;21:1156-63 pubmed 出版商
  • 免疫印迹; brewer's yeast; 图 6
Wu G, Huang C, Yu Y. Pseudouridine in mRNA: Incorporation, Detection, and Recoding. Methods Enzymol. 2015;560:187-217 pubmed 出版商
  • 免疫印迹; 人类; 1:10,000; 图 1.a,b
Treacy Abarca S, Mukherjee S. Legionella suppresses the host unfolded protein response via multiple mechanisms. Nat Commun. 2015;6:7887 pubmed 出版商
  • 免疫印迹; 人类
Siriwardana N, Meyer R, Panchenko M. The novel function of JADE1S in cytokinesis of epithelial cells. Cell Cycle. 2015;14:2821-34 pubmed 出版商
  • 免疫印迹; 人类; 图 4
Hoover H, Li J, Marchese J, Rothwell C, Borawoski J, Jeffery D, et al. Quantitative Proteomic Verification of Membrane Proteins as Potential Therapeutic Targets Located in the 11q13 Amplicon in Cancers. J Proteome Res. 2015;14:3670-9 pubmed 出版商
  • 免疫印迹; 小鼠; 1:5000; 图 s3
Li F, Buck D, De Winter J, Kolb J, Meng H, Birch C, et al. Nebulin deficiency in adult muscle causes sarcomere defects and muscle-type-dependent changes in trophicity: novel insights in nemaline myopathy. Hum Mol Genet. 2015;24:5219-33 pubmed 出版商
  • 免疫印迹; 幽门螺杆菌; 1:5000
Yuan Y, Wu Q, Cheng G, Liu X, Liu S, Luo J, et al. Recombinant human lactoferrin enhances the efficacy of triple therapy in mice infected with Helicobacter pylori. Int J Mol Med. 2015;36:363-8 pubmed 出版商
  • 免疫印迹; fission yeast
Hodges A, Gallegos I, Laughery M, Meas R, Tran L, Wyrick J. Histone Sprocket Arginine Residues Are Important for Gene Expression, DNA Repair, and Cell Viability in Saccharomyces cerevisiae. Genetics. 2015;200:795-806 pubmed 出版商
  • 免疫印迹; 人类
Wright J, Atwan Z, Morris S, Leppard K. The Human Adenovirus Type 5 L4 Promoter Is Negatively Regulated by TFII-I and L4-33K. J Virol. 2015;89:7053-63 pubmed 出版商
  • 免疫印迹; 人类
Pasqualon T, Pruessmeyer J, Weidenfeld S, Babendreyer A, Groth E, Schumacher J, et al. A transmembrane C-terminal fragment of syndecan-1 is generated by the metalloproteinase ADAM17 and promotes lung epithelial tumor cell migration and lung metastasis formation. Cell Mol Life Sci. 2015;72:3783-801 pubmed 出版商
  • 免疫印迹; 大鼠; 图 1
Moreira J, Wohlwend M, Alves M, Wisløff U, Bye A. A small molecule activator of AKT does not reduce ischemic injury of the rat heart. J Transl Med. 2015;13:76 pubmed 出版商
  • 免疫印迹; 小鼠
Richardson E, Shukla S, Sweet D, Wearsch P, Tsichlis P, Boom W, et al. Toll-like receptor 2-dependent extracellular signal-regulated kinase signaling in Mycobacterium tuberculosis-infected macrophages drives anti-inflammatory responses and inhibits Th1 polarization of responding T cells. Infect Immun. 2015;83:2242-54 pubmed 出版商
  • 免疫印迹; 鸡
Jarosinski K, Donovan K, Du G. Expression of fluorescent proteins within the repeat long region of the Marek's disease virus genome allows direct identification of infected cells while retaining full pathogenicity. Virus Res. 2015;201:50-60 pubmed 出版商
  • 免疫印迹; 小鼠; 图 4
Radhakrishnan V, Kojs P, Ramalingam R, Midura Kiela M, Angeli P, Kiela P, et al. Experimental colitis is associated with transcriptional inhibition of Na+/Ca2+ exchanger isoform 1 (NCX1) expression by interferon γ in the renal distal convoluted tubules. J Biol Chem. 2015;290:8964-74 pubmed 出版商
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Liu L, Zou P, Zheng L, Linarelli L, Amarell S, Passaro A, et al. Tamoxifen reduces fat mass by boosting reactive oxygen species. Cell Death Dis. 2015;6:e1586 pubmed 出版商
  • 免疫印迹; 人类; 1:3000; 图 1,2,3,4
Zhang P, Wang L, Rodriguez Aguayo C, Yuan Y, Debeb B, Chen D, et al. miR-205 acts as a tumour radiosensitizer by targeting ZEB1 and Ubc13. Nat Commun. 2014;5:5671 pubmed 出版商
  • 免疫印迹; 人类; 25 ng/ml; 图 4
Freund A, Zhong F, Venteicher A, Meng Z, Veenstra T, Frydman J, et al. Proteostatic control of telomerase function through TRiC-mediated folding of TCAB1. Cell. 2014;159:1389-403 pubmed 出版商
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Tan X, Peng J, Fu Y, An S, Rezaei K, Tabbara S, et al. miR-638 mediated regulation of BRCA1 affects DNA repair and sensitivity to UV and cisplatin in triple-negative breast cancer. Breast Cancer Res. 2014;16:435 pubmed 出版商
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Sarkar J, Simanian E, Tuggy S, Bartlett J, Snead M, Sugiyama T, et al. Comparison of two mouse ameloblast-like cell lines for enamel-specific gene expression. Front Physiol. 2014;5:277 pubmed 出版商
  • 免疫印迹; 小鼠; 1:3000; 图 4c
  • 免疫印迹; 人类; 1:3000; 图 1a
Zhang P, Wei Y, Wang L, Debeb B, Yuan Y, Zhang J, et al. ATM-mediated stabilization of ZEB1 promotes DNA damage response and radioresistance through CHK1. Nat Cell Biol. 2014;16:864-75 pubmed 出版商
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Walker M, Volta M, Cataldi S, Dinelle K, Beccano Kelly D, Munsie L, et al. Behavioral deficits and striatal DA signaling in LRRK2 p.G2019S transgenic rats: a multimodal investigation including PET neuroimaging. J Parkinsons Dis. 2014;4:483-98 pubmed 出版商
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Huether R, Dong L, Chen X, Wu G, Parker M, Wei L, et al. The landscape of somatic mutations in epigenetic regulators across 1,000 paediatric cancer genomes. Nat Commun. 2014;5:3630 pubmed 出版商
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Chen J, Solomides C, Simpkins H. Sensitization of mesothelioma cells to platinum-based chemotherapy by GST? knockdown. Biochem Biophys Res Commun. 2014;447:77-82 pubmed 出版商
  • 免疫印迹; 小鼠
Surma M, Handy C, Chang J, Kapur R, Wei L, Shi J. ROCK1 deficiency enhances protective effects of antioxidants against apoptosis and cell detachment. PLoS ONE. 2014;9:e90758 pubmed 出版商
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Zha R, Guo W, Zhang Z, Qiu Z, Wang Q, Ding J, et al. Genome-wide screening identified that miR-134 acts as a metastasis suppressor by targeting integrin ?1 in hepatocellular carcinoma. PLoS ONE. 2014;9:e87665 pubmed 出版商
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Cheng M, Huang M, Lin C, Lin L, Lee H, Jiang J, et al. Expression of matriptase correlates with tumour progression and clinical prognosis in oral squamous cell carcinoma. Histopathology. 2014;65:24-34 pubmed 出版商
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Pritchard T, Kawase Y, Haghighi K, Anjak A, Cai W, Jiang M, et al. Active inhibitor-1 maintains protein hyper-phosphorylation in aging hearts and halts remodeling in failing hearts. PLoS ONE. 2013;8:e80717 pubmed 出版商
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Zhang J, Zhang P, Wei Y, Piao H, Wang W, Maddika S, et al. Deubiquitylation and stabilization of PTEN by USP13. Nat Cell Biol. 2013;15:1486-1494 pubmed 出版商
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Kimura R, Kasamatsu A, Koyama T, Fukumoto C, Kouzu Y, Higo M, et al. Glutamate acid decarboxylase 1 promotes metastasis of human oral cancer by ?-catenin translocation and MMP7 activation. BMC Cancer. 2013;13:555 pubmed 出版商
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Park J, Kim S, Kim K, Jin C, Choi K, Jang J, et al. Inhibition of notch signalling ameliorates experimental inflammatory arthritis. Ann Rheum Dis. 2015;74:267-74 pubmed 出版商
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Singh V, Rubinstein J, Arvanitis D, Ren X, Gao X, Haghighi K, et al. Abnormal calcium cycling and cardiac arrhythmias associated with the human Ser96Ala genetic variant of histidine-rich calcium-binding protein. J Am Heart Assoc. 2013;2:e000460 pubmed 出版商
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Cooper L, Li W, Lu Y, Centracchio J, Terentyeva R, Koren G, et al. Redox modification of ryanodine receptors by mitochondria-derived reactive oxygen species contributes to aberrant Ca2+ handling in ageing rabbit hearts. J Physiol. 2013;591:5895-911 pubmed 出版商
  • 免疫印迹; 酵母菌目
Kennedy J, Daughdrill G, Schmidt K. A transient ?-helical molecular recognition element in the disordered N-terminus of the Sgs1 helicase is critical for chromosome stability and binding of Top3/Rmi1. Nucleic Acids Res. 2013;41:10215-27 pubmed 出版商
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Tykwinska K, Lauster R, Knaus P, Rosowski M. Growth and differentiation factor 3 induces expression of genes related to differentiation in a model of cancer stem cells and protects them from retinoic acid-induced apoptosis. PLoS ONE. 2013;8:e70612 pubmed 出版商
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Shim J, Greenblatt M, Zou W, Huang Z, Wein M, Brady N, et al. Schnurri-3 regulates ERK downstream of WNT signaling in osteoblasts. J Clin Invest. 2013;123:4010-22 pubmed 出版商
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Cieply B, Farris J, Denvir J, Ford H, Frisch S. Epithelial-mesenchymal transition and tumor suppression are controlled by a reciprocal feedback loop between ZEB1 and Grainyhead-like-2. Cancer Res. 2013;73:6299-309 pubmed 出版商
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Zhang Z, Zhu Y, Lai Y, Wu X, Feng Z, Yu Y, et al. Follicle-stimulating hormone inhibits apoptosis in ovarian cancer cells by regulating the OCT4 stem cell signaling pathway. Int J Oncol. 2013;43:1194-204 pubmed 出版商
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Novo R, Azevedo P, Minicucci M, Zornoff L, Paiva S. Effect of beta-carotene on oxidative stress and expression of cardiac connexin 43. Arq Bras Cardiol. 2013;101:233-9 pubmed 出版商
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Martínez Gil L, Alamares Sapuay J, Ramana Reddy M, Goff P, Premkumar Reddy E, Palese P. A small molecule multi-kinase inhibitor reduces influenza A virus replication by restricting viral RNA synthesis. Antiviral Res. 2013;100:29-37 pubmed 出版商
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Cen J, Wu H, Wang J, Ren X, Zhang H, Wang J, et al. Local injection of lentivirus encoding LINGO-1-shRNA promotes functional recovery in rats with complete spinal cord transection. Spine (Phila Pa 1976). 2013;38:1632-9 pubmed 出版商
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Jia C, Li X, Sun H, Sui L. Over-expression of the overexpressed in lung cancer 1 is associated with poor prognosis in epithelial ovarian cancer. J Surg Oncol. 2013;107:847-52 pubmed 出版商
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Hu J, Wang B, Fang X, Means W, McCormick R, Gomelsky M, et al. c-di-GMP signaling regulates E. coli O157:H7 adhesion to colonic epithelium. Vet Microbiol. 2013;164:344-51 pubmed 出版商
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Moreira V, Salvador J, Simoes S, Destro F, Gavioli R. Novel oleanolic vinyl boronates: synthesis and antitumor activity. Eur J Med Chem. 2013;63:46-56 pubmed 出版商
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Kong L, Li S, Liao Q, Zhang Y, Sun R, Zhu X, et al. Oleanolic acid and ursolic acid: novel hepatitis C virus antivirals that inhibit NS5B activity. Antiviral Res. 2013;98:44-53 pubmed 出版商
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Chen L, Lin C, Chan T, Chow R, Song Y, Liu M, et al. Recoding RNA editing of AZIN1 predisposes to hepatocellular carcinoma. Nat Med. 2013;19:209-16 pubmed 出版商
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Goodwin J, Feng Y, Velazquez H, Sessa W. Endothelial glucocorticoid receptor is required for protection against sepsis. Proc Natl Acad Sci U S A. 2013;110:306-11 pubmed 出版商
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Sakagami Y, Kudo T, Tanimukai H, Kanayama D, Omi T, Horiguchi K, et al. Involvement of endoplasmic reticulum stress in tauopathy. Biochem Biophys Res Commun. 2013;430:500-4 pubmed 出版商
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Wu H, Cen J, Zhong Q, Chen L, Wang J, Deng D, et al. The promotion of functional recovery and nerve regeneration after spinal cord injury by lentiviral vectors encoding Lingo-1 shRNA delivered by Pluronic F-127. Biomaterials. 2013;34:1686-700 pubmed 出版商
  • 免疫印迹; 人类; 1:100
Vazquez Tello A, Halwani R, Hamid Q, Al Muhsen S. Glucocorticoid receptor-beta up-regulation and steroid resistance induction by IL-17 and IL-23 cytokine stimulation in peripheral mononuclear cells. J Clin Immunol. 2013;33:466-78 pubmed 出版商
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Gündisch S, Boeckeler E, Behrends U, Amtmann E, Ehrhardt H, Jeremias I. Glucocorticoids augment survival and proliferation of tumor cells. Anticancer Res. 2012;32:4251-61 pubmed
  • 免疫印迹; 人类
Kong D, Ahmad A, Bao B, Li Y, Banerjee S, Sarkar F. Histone deacetylase inhibitors induce epithelial-to-mesenchymal transition in prostate cancer cells. PLoS ONE. 2012;7:e45045 pubmed
  • 免疫印迹; 大鼠; 1:10000
O Donnell J, Kalichira A, Bi J, Lewandowski E. In vivo, cardiac-specific knockdown of a target protein, malic enzyme-1, in rat via adenoviral delivery of DNA for non-native miRNA. Curr Gene Ther. 2012;12:454-62 pubmed
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图像 1 :
赛默飞世尔 MA5-15738 图像 1
1:2000 (0.5ug/mL) Ab dilution used in WB of 5ug/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6).
图像 2 :
赛默飞世尔 MA5-15738 图像 2
WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3)
图像 3 :
赛默飞世尔 MA5-15738 图像 3
Western blot analysis of GAPDH was performed by loading 20ug of THP-1 whole cell lysates per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% milk in TBST. The membrane was probed with a GAPDH loading control monoclonal antibody (Product # MA5-15738) at a dilution of 1:5000 overnight at 4ºC, washed in TBST, and probed with a HRP-conjugated goat anti-mouse IgG at a dilution of 1:40,000 for 1 hr at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
产品类型 :
产品名称 :
GAPDH Loading Control Monoclonal Antibody (GA1R)
目录# :
规格 :
价格 :
克隆性 :
纯度 :
宿主 :
反应物种 :
细菌, 狗, 鸡, 仓鼠, 人类, 昆虫, 小鼠, 兔, 大鼠, 酵母
应用 :
ELISA: Assay Dependent, Flow Cytometry: 2 µg/test, Immunocytochemistry: 1:20-1:200, Immunofluorescence: 1:20-1:200, Immunohistochemistry (Paraffin): 1:50-1:500, Western Blot: 1:1000-1:10,000
物种 :
细菌, 狗, 鸡, 仓鼠, 人类, 昆虫, 小鼠, 兔, 大鼠, 酵母
克隆 :
抗体亚型 :
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
免疫原 :
格式 :
应用w/稀释 :
ELISA: Assay Dependent, Flow Cytometry: 2 µg/test, Immunocytochemistry: 1:20-1:200, Immunofluorescence: 1:20-1:200, Immunohistochemistry (Paraffin): 1:50-1:500, Western Blot: 1:1000-1:10,000
别名 :
38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; bb02e05; cb350; cb609; CDABP0047; EC; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; fb71f08; fk58c09; G3PD; G3PDH; Gapd; GAPD2; gapdh; GAPDH2; GAPDH-2; GAPDHS; GAPDS; Gapd-s; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; glyceraldehyde phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase GAPDH; glyceraldehyde-3-phosphate dehydrogenase like-17 protein; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; Glyceraldehyde-3-phosphate dehydrogenase, testis-specific; glyceraldehyde-phosphate-dehydrogenase; glycerine aldehyde 3-phosphate dehydrogenase; HEL-S-162eP; HEL-S-278; HGNC:4141; HSD35; HSD-35; I79_001391; KNC-NDS6; LOW QUALITY PROTEIN: glyceraldehyde-3-phosphate dehydrogenase, testis-specific; mg:bb02e05; MGC128279 protein; MGC88685; OK/SW-cl.12; Peptidyl-cysteine S-nitrosylase GAPDH; similar to glyceraldehyde 3-phosphate dehydrogenase; spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; Unknown (protein for IMAGE:8101613); wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908
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公司总部: 美国