产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
GRK2单克隆抗体(3F8)
目录 :
MA5-15840
规格 :
100微升
价格 :
美国516
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
3F8
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 2
出版应用/物种/样本/稀释参考文献
  • 免疫组化-石蜡切片; 人类; 1:1000
Buchholz M, Honstein T, Kirchhoff S, Kreider R, Schmidt H, Sipos B, et al. A multistep high-content screening approach to identify novel functionally relevant target genes in pancreatic cancer. PLoS ONE. 2015;10:e0122946 pubmed 出版商
Mayberry C, Wilczek M, Fong T, Nichols S, Maginnis M. GRK2 mediates β-arrestin interactions with 5-HT2 receptors for JC polyomavirus endocytosis. J Virol. 2021;: pubmed 出版商
图像
图像 1 :
赛默飞世尔 MA5-15840 图像 1
Immunofluorescence analysis of NIH/3T3 cells using MA5-15840 anti-GRK2 antibody (green). Blue staining is DRAQ5 fluorescent DNA dye.
图像 2 :
赛默飞世尔 MA5-15840 图像 2
Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues (left) and cervical cancer tissues (right) using MA5-15840 anti-GRK2 antibody with DAB staining.
产品信息
产品类型 :
抗体
产品名称 :
GRK2单克隆抗体(3F8)
目录# :
MA5-15840
规格 :
100微升
价格 :
美国516
克隆性 :
单克隆
宿主 :
小鼠
反应物种 :
Human, Mouse, Non-human primate, Rat
应用 :
Immunocytochemistry: 1:200-1:1000, Immunofluorescence: 1/200 - 1/1000, Immunohistochemistry: 1/200 - 1/1000, Western Blot: 1/500 - 1/2000
物种 :
Human, Mouse, Non-human primate, Rat
克隆 :
3F8
抗体亚型 :
IgG1
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.
免疫原 :
纯化重组片段对应于人GRK2在大肠杆菌中表达
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:200-1:1000, Immunofluorescence: 1/200 - 1/1000, Immunohistochemistry: 1/200 - 1/1000, Western Blot: 1/500 - 1/2000
别名 :
ADRBK1; Adrbk-1; adrenergic beta receptor kinase 1; adrenergic receptor kinase, beta 1; adrenergic, beta, receptor kinase 1; BARK; BARK1; Bark-1; beta ARK; Beta-adrenergic receptor kinase 1; beta-adrenergic receptor kinase 1 beta ARK1; beta-AR kinase-1; betaARK1; BETA-ARK1; beta-ARK-1; EC 2.7.1.126; G protein-coupled receptor kinase 2; G-protein coupled receptor kinase 2; G-protein-coupled receptor kinase 2; grk 2; Grk2; GRK-2
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国