产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
beta Tubulin Loading Control Monoclonal Antibody (BT7R), HRP
目录 :
MA5-16308-HRP
规格 :
50微升
价格 :
美国235.00
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
HRP
克隆名称 :
BT7R
反应物种 :
人类, 小鼠, 大鼠, 犬, 鸡, domestic rabbit
应用 :
免疫印迹
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 6
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 大鼠; 1:5000; 图 4a
Thierry M, Pasquis B, Buteau B, Fourgeux C, Dembele D, Leclère L, et al. Early adaptive response of the retina to a pro-diabetogenic diet: Impairment of cone response and gene expression changes in high-fructose fed rats. Exp Eye Res. 2015;135:37-46 pubmed 出版商
Nuti E, Rossello A, Cuffaro D, Camodeca C, Van Bael J, van der Maat D, et al. Bivalent Inhibitor with Selectivity for Trimeric MMP-9 Amplifies Neutrophil Chemotaxis and Enables Functional Studies on MMP-9 Proteoforms. Cells. 2020;9: pubmed 出版商
de la Fuente L, Arzalluz Luque Á, Tardáguila M, Del Risco H, Martí C, Tarazona S, et al. tappAS: a comprehensive computational framework for the analysis of the functional impact of differential splicing. Genome Biol. 2020;21:119 pubmed 出版商
Bezawork Geleta A, Wen H, Dong L, Yan B, Vider J, Boukalova S, et al. Alternative assembly of respiratory complex II connects energy stress to metabolic checkpoints. Nat Commun. 2018;9:2221 pubmed 出版商
Bilal S, Jaggi S, Janosevic D, Shah N, Teymour S, Voronina A, et al. ZO-1 protein is required for hydrogen peroxide to increase MDCK cell paracellular permeability in an ERK 1/2-dependent manner. Am J Physiol Cell Physiol. 2018;315:C422-C431 pubmed 出版商
Allouche J, Bellon N, Saidani M, Stanchina Chatrousse L, Masson Y, Patwardhan A, et al. In vitro modeling of hyperpigmentation associated to neurofibromatosis type 1 using melanocytes derived from human embryonic stem cells. Proc Natl Acad Sci U S A. 2015;112:9034-9 pubmed 出版商
图像
图像 1 :
赛默飞世尔 MA5-16308-HRP 图像 1
Western blot analysis of Beta-Tubulin was performed by loading 50ug of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Beta-Tubulin monoclonal antibody (Product # MA5-16308-HRP) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
产品信息
产品类型 :
抗体
产品名称 :
beta Tubulin Loading Control Monoclonal Antibody (BT7R), HRP
目录# :
MA5-16308-HRP
规格 :
50微升
价格 :
美国235.00
克隆性 :
单克隆
纯度 :
蛋白质A
宿主 :
小鼠
反应物种 :
Canine, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat
应用 :
Western Blot: 1 µg/mL
物种 :
Canine, Chicken, Human, Mouse, Non-human primate, Rabbit, Rat
克隆 :
BT7R
抗体亚型 :
IgG2a
储存 :
4° C
描述 :
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
免疫原 :
KLH conjugated peptide of the N-terminus of Beta-Tubulin.
格式 :
液体
应用w/稀释 :
Western Blot: 1 µg/mL
别名 :
2310057H16Rik; 2810484G07Rik; 3200002H15Rik; AA408537; AI325297; AI596182; B130022C14Rik; bA631M21.2; BB220206; beta 5-tubulin; beta Ib tubulin; beta tubulin; beta tubulin 1; beta-4; beta-4 tubulin; beta-4-tubulin; beta-5; beta-5 tubulin; beta5-tubulin; betaIII-tubulin; beta-tubulin; beta-tubulin class-III; Beta-tubulin class-V; beta-tubulin class-VI; Beta-tubulin isotype I; c(beta)7 tubulin; CDCBM; CDCBM1; CDCBM5; CDCBM6; CFEOM3; CFEOM3A; class I beta tubulin; Class I beta-tubulin; class IIa beta-tubulin; class III beta-tubulin; class IVa beta-tubulin; class V beta tubulin; class V beta-tubulin; CSCSC1; dJ40E16.7; dystonia 4, torsion (autosomal dominant); DYT4; FEOM3; HSA10p15 beta-tubulin 4Q; HsT1601; hypothetical protein LOC540236; hypothetical protein LOC550458; I79_000194; I79_012097; LOC100349792; LOC100761889; LOW QUALITY PROTEIN: tubulin beta-6 chain; M(beta)1; M(beta)2; M(beta)3; M(beta)4; M(beta)5; M(beta)6; M40; M40 antibody; MGC128415 protein; MGC137802 protein; neuron-specific class III beta-tubulin; OK/SW-cl.56; OOMD; OOMD2; RGD1305887; si:ch211-122i22.4; si:ch211-9a18.2; Tubb; tubb beta-5; tubb.L; Tubb1; TUBB2; Tubb2a; tubb2a {ECO:0000250; Tubb3; TUBB4; TUBB4A; tubb5; TUBB-5; TUBB6; TUBB8; Tubulin 5 beta; tubulin beta; tubulin beta 1 class VI; tubulin beta 2A class IIa; tubulin beta 3 class III; tubulin beta 4'; tubulin beta 4A class IVa; tubulin beta 6 class V; tubulin beta 8 class VIII; Tubulin beta chain; tubulin beta class I; tubulin beta class I L homeolog; Tubulin beta class IIa; Tubulin beta class V; tubulin beta MGC4083; tubulin beta-1; tubulin beta-1 chain; tubulin beta-1 chain-like; tubulin beta-2A chain; tubulin beta-2B chain; tubulin beta-3 chain; Tubulin beta-4 chain; tubulin beta-4A chain; Tubulin beta-5 chain; tubulin beta-5 chain; tubulin beta-6 chain; tubulin beta-6 chain; Tubulin beta-7 chain; tubulin beta-8 chain; Tubulin beta-III; tubulin, beta 1 class VI; tubulin, beta 2; tubulin, beta 2A; tubulin, beta 2A class IIA; tubulin, beta 3; tubulin, beta 3 class III; tubulin, beta 4; tubulin, beta 4 class IVa; tubulin, beta 4A class IVa; tubulin, beta 5; tubulin, beta 5 class I; tubulin, beta 6 class V; tubulin, beta class I; tubulin, beta family 1; tubulin, beta polypeptide; tubulin, beta polypeptide 2; tubulin, beta, 3; tubulin, beta, 4; tubulin, beta, 5; uncharacterized protein LOC550458; UniProtKB:Q7TMM9}; Unknown (protein for MGC:134115); unnamed protein product; XELAEV_18039222mg; XLOT; zbetaI; zgc:112515
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国