产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
F4/80单克隆抗体(SP115)
目录 :
MA5-16363
规格 :
500微升
价格 :
美国497.00
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
SP115
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 10
出版应用/物种/样本/稀释 | 参考文献 |
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图像
图像 1 :
Immunohistochemistry was performed on formalin -fixed paraffin-embedded rat infarct heart tissue sections. To expose target proteins, heat-induced epitope retrieval (HIER) was performed in sodium citrate buffer (pH 6.0) for 30 minutes at 100?C. Tissues were blocked in 10% normal goat serum for 20 minutes at room temperature and probed with a F4/80 monoclonal antibody (Product # MA5-16363) at a dilution of 1:100 for 1 hour at room temperature. Tissues were washed extensively with 1X PBS. Detection was performed using a biotinylated goat anti-rabbit IgG secondary antibody followed by an avidin-biotin complex reagent and DAB colorimetric substrate. Tissues were counterstained with hematoxilyn and visualized by light microscopy. Data courtesy of the Innovators Program.
图像 2 :
Immunohistochemical analysis of F4/80 using anti-F4/80 Monoclonal Antibody (MA5-16363) in Mouse Liver Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
产品信息
产品类型 :
抗体
产品名称 :
F4/80单克隆抗体(SP115)
目录# :
MA5-16363
规格 :
500微升
价格 :
美国497.00
克隆性 :
单克隆
纯度 :
蛋白质A/G
宿主 :
兔
反应物种 :
小鼠
应用 :
Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:100
物种 :
小鼠
克隆 :
SP115
抗体亚型 :
IgG
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
免疫原 :
Synthetic peptide derived from N-terminus of mouse F4/80 protein.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:100, Immunohistochemistry (Paraffin): 1:100
别名 :
ADGRE1; Adhesion G protein-coupled receptor E1; Adhesion G protein-coupled receptor E2; cell surface glycoprotein EMR1; cell surface glycoprotein F4/80; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like 1; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr1; EMR1 hormone receptor; F4/80; Gpf480; Ly71; lymphocyte antigen 71; TM7LN3
更多信息或购买 :
公司信息
赛默飞世尔