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抗体

A型流感H1N1 NS1单克隆抗体(3G1)

MA5-33229

100微升

375美元

单克隆

小鼠

未共轭

3G1

免疫印迹, 酶联免疫吸附测定

A型流感H1N1 NS1单克隆抗体(3G1)

MA5-33229

100微升

375美元

单克隆

IgG分馏成分

小鼠

病毒

ELISA: 1 µL/mg, Western Blot: 1 µL/mL

病毒

3G1

IgM

-20°C

Influenza A H1N1 NS1. Non-structural protein 1 inhibits post-transcriptional processing of cellular pre-mRNA, by binding and inhibiting two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs: the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). This results in the accumulation of unprocessed 3' end pre-mRNAs which can't be exported from the nucleus. Cellular protein synthesis is thereby shut off very early after virus infection. Viral protein synthesis is not affected by the inhibition of the cellular 3' end processing machinery because the poly(A) tails of viral mRNAs are produced by the viral polymerase through a stuttering mechanism. NS1 also prevents the establishment of the cellular antiviral state by inhibiting TRIM25-mediated DDX58 ubiquitination, which normally triggers the antiviral transduction signal that leads to the activation of type I IFN genes by transcription factors like IRF3 and IRF7. Prevents human EIF2AK2/PKR activation, either by binding double-strand RNA, or by interacting directly with EIF2AK2/PKR. This function may be important at the very beginning of the infection, when NS1 is mainly present in the cytoplasm. Also binds poly(A) and U6 snRNA. Suppresses the RNA silencing-based antiviral response in Drosophila cells.

合成肽

液体

ELISA: 1 µL/mg, Western Blot: 1 µL/mL

A型流感