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抗体

NEFM单克隆抗体(RNF 406)

OMA1-06111

100µL

美国345

单克隆

小鼠

未共轭

RNF 406

中国人仓鼠, 热带爪蟾, 豚鼠, 人类, 大鼠, 非洲爪蛙, domestic rabbit

免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片, 免疫组化-冰冻切片

NEFM单克隆抗体(RNF 406)

OMA1-06111

100µL

美国345

单克隆

纯化

小鼠

Guinea Pig, Hamster, Human, Non-human primate, Rabbit, Rat, Xenopus laevis

Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry (Frozen): 1:50-1:100, Immunohistochemistry (Paraffin): 1:50-1:100, Western Blot: 1:100 - 1:500

Guinea Pig, Hamster, Human, Non-human primate, Rabbit, Rat, Xenopus laevis

RNF 406

IgG1

短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Neurofilament preparation of calf brain tissue

液体

Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry (Frozen): 1:50-1:100, Immunohistochemistry (Paraffin): 1:50-1:100, Western Blot: 1:100 - 1:500

160 kDa neurofilament protein; BOS_9093; major neuronal intermediate filament subunit; middle molecular weight neurofilament protein NF-M(2); Nef3; NEFM; nef-m; nefm.S; nefm-a; nefm-b; Neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; neurofilament M subunit; neurofilament medium; Neurofilament medium polypeptide; neurofilament medium S homeolog; neurofilament protein M; Neurofilament protein, middle polypeptide; Neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide S homeolog; neurofilament-3 (150 kD medium); neurofilament-M; NF160; NF165; NFM; NF-M; NF-M c-terminus; NF-M protein; NMC; XELAEV_18020229mg