产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
NPM1多克隆抗体
目录 :
PA1-029
规格 :
100微克
价格 :
美国480
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Western blot analysis of Nucleophosmin (Pierce Product # PA1-029) was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing Nucleophosmin at a dilution of 1:500 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Pierce Product # 31460) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product #34077).
产品信息
产品类型 :
抗体
产品名称 :
NPM1多克隆抗体
目录# :
PA1-029
规格 :
100微克
价格 :
美国480
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
兔
反应物种 :
Human, Mouse, Non-human primate, Rat
应用 :
Immunocytochemistry: 1:100, Immunofluorescence: 1:100, Immunohistochemistry: 1:200, Western Blot: 1:500 - 1:1000
物种 :
Human, Mouse, Non-human primate, Rat
抗体亚型 :
IgG
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.
免疫原 :
合成多肽对应于氨基酸残基23-38和226-240人的NPM
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:100, Immunofluorescence: 1:100, Immunohistochemistry: 1:200, Western Blot: 1:500 - 1:1000
别名 :
B23; B23 antibody; B23NP; MGC104254; mNPM antibody; mNPM1 antibody; NO38; NO38 antibody; NPM; Npm1; Nucleolar phosphoprotein B23; nucleolar protein B23.1; Nucleolar protein NO38; nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin 1; nucleophosmin/nucleoplasmin family, member 1; numatrin; numatrin antibody; testicular tissue protein Li 128
更多信息或购买 :
公司信息
赛默飞世尔