产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
ALG1多克隆抗体
目录 :
PA1-031
规格 :
100微克
价格 :
美国430
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
roundworm
应用 :
免疫印迹
更多信息或购买 :
文章摘录数: 4
参考文献
Seth P, Premont R, Stamler J. An optimized protocol for isolation of S-nitrosylated proteins from C. elegans. STAR Protoc. 2021;2:100547 pubmed 出版商
Broughton J, Pasquinelli A. Detection of microRNA-Target Interactions by Chimera PCR (ChimP). Methods Mol Biol. 2018;1823:153-165 pubmed 出版商
Inukai S, Pincus Z, de Lencastre A, Slack F. A microRNA feedback loop regulates global microRNA abundance during aging. RNA. 2017;: pubmed 出版商
Chu Y, Chen H, Huang T, Chan S. A novel function for the DEAD-box RNA helicase DDX-23 in primary microRNA processing in Caenorhabditis elegans. Dev Biol. 2016;409:459-72 pubmed 出版商
图像
图像 1 :
赛默飞世尔 PA1-031 图像 1
Western blot analysis of Alg-1 was performed by loading 15µg WT or Alg-1 (-) worm extract onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a rabbit polyclonal antibody recognizing Alg-1 (Product # PA1-031) at a dilution of 1:5000 for 1 hour and room temperature on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product #31460) at a dilution of 1:15000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico (Product #34077).
产品信息
产品类型 :
抗体
产品名称 :
ALG1多克隆抗体
目录# :
PA1-031
规格 :
100微克
价格 :
美国430
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
反应物种 :
C. elegans
应用 :
Western Blot: 1:5000
物种 :
C. elegans
抗体亚型 :
IgG
储存 :
-20°C
描述 :
The enzyme encoded by this gene catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. This gene is mutated in congenital disorder of glycosylation type Ik.
免疫原 :
肽序列DLEEIPNSPPTQPQTFSDVPQR
格式 :
液体
应用w/稀释 :
Western Blot: 1:5000
别名 :
ALG1; alg-1; ALG1, chitobiosyldiphosphodolichol beta-mannosyltransferase; Argonaute; Argonaute-like; asparagine-linked glycosylation 1 (beta-1,4-mannosyltransferase); asparagine-linked glycosylation 1 homolog (S. cerevisiae, beta-1,4-mannosyltransferase); asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase); asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog; asparagine-linked glycosylation protein 1 homolog; beta-1,4 mannosyltransferase; beta-1,4-mannosyltransferase; CDG1K; CELE_F48F7.1; Chitobiosyldiphosphodolichol beta-mannosyltransferase; GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase; GDP-mannose-dolichol diphosphochitobiose mannosyltransferase; HMAT1; hMat-1; HMT1; HMT-1; Mannosyltransferase-1; Mat-1; MT-1; Protein argonaute; protein-coding; PSEC0061; UNQ861/PRO1870
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国