产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
Cdc42多克隆抗体
目录 :
PA1-092X
规格 :
20微升
价格 :
美国169
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 7
| 出版应用/物种/样本/稀释 | 参考文献 |
|---|---|
| |
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图像
图像 1 :
Immunofluorescent analysis of cdc42 in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a cdc42 polyclonal antibody (Product # PA1-092) at a dilution of 1:200 overnight at 4 ºC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). cdc42 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
图像 2 :
Western blot analysis of cdc42 was performed by loading 50ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a rabbit polyclonal antibody recognizing cdc42 (Product # PA1-092) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product #31460) at a dilution of 1:10000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Pico (Product #34080).
产品信息
产品类型 :
抗体
产品名称 :
Cdc42多克隆抗体
目录# :
PA1-092X
规格 :
20微升
价格 :
美国169
克隆性 :
多克隆
纯度 :
蛋白质A
宿主 :
兔
反应物种 :
Human, Mouse, Non-human primate, Rat
应用 :
Immunocytochemistry: 1:100, Immunofluorescence: 1:100, Immunohistochemistry (Paraffin): 1:20, Western Blot: 1:1000
物种 :
Human, Mouse, Non-human primate, Rat
抗体亚型 :
IgG
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for the Rac1 gene.
免疫原 :
C-Terminal truncated human recombinant protein
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:100, Immunofluorescence: 1:100, Immunohistochemistry (Paraffin): 1:20, Western Blot: 1:1000
别名 :
AI747189; AU018915; CDC42; CDC42Hs; Cell division control protein 42 homolog; cell division cycle 42; cell division cycle 42 (GTP binding protein); cell division cycle 42 homolog; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K; G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; GTP-binding protein; RP1-224A6.5; small GTP binding protein CDC42; TKS
更多信息或购买 :
公司信息
赛默飞世尔