产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
Nanog Polyclonal Antibody
目录 :
PA1-097
规格 :
100微克
价格 :
美国470
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
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文章摘录数: 13
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图像
图像 1 :
Immunofluorescent analysis of Nanog using anti-Nanog polyclonal antibody (Product# PA1-097) shows nuclear localization in the human iPS cell line HEL 11.4. Nanog staining (red) and an overlay image of Nanog with DAPI (pink) is shown. HEL-11.4 cells were grown on matrigel coated chamber slides, fixed with formaldehyde and permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. The cells were probed with a rabbit polyclonal antibody recognizing Nanog (Product# PA1-097) at a dilution of 1:100 overnight at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken on at 20X magnification.
图像 2 :
Immunofluorescent analysis of Nanog using anti-Nanog polyclonal antibody (Product# PA1-097) shows nuclear localization in the human embryonic stem cell line H9. Nanog staining (red) and an overlay image of Nanog with DAPI (pink) is shown. H9 cells were grown on matrigel coated chamber slides, fixed with formaldehyde and permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. The cells were probed with a rabbit polyclonal antibody recognizing Nanog (Product# PA1-097) at a dilution of 1:100 overnight at 4 ºC, washed with PBS and incubated with a fluorescein conjugated secondary antibody at a dilution of 1:100 for 60 minutes at room temperature. Images were taken on at 20X magnification.
图像 3 :
Western blot analysis of Nanog was performed by loading 80ug of NCCIT, NTERA-2 and negative control HeLa whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for 1 hour. Membranes were probed with a rabbit polyclonal antibody recognizing Nanog (Product # PA1-097) at a dilution of 1:2000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product # 31460) at a dilution of 1:20,000 for one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product #34078).
产品信息
产品类型 :
抗体
产品名称 :
Nanog Polyclonal Antibody
目录# :
PA1-097
规格 :
100微克
价格 :
美国470
克隆性 :
多克隆
纯度 :
蛋白质A
宿主 :
兔
反应物种 :
人类
应用 :
ChIP assay: 1-3 µL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry (Paraffin): 1:100-1:1000, Western Blot: 1:1000
物种 :
人类
抗体亚型 :
IgG
储存 :
-20°C
描述 :
NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.
免疫原 :
Full-length human recombinant protein expressed in bacteria
格式 :
液体
应用w/稀释 :
ChIP assay: 1-3 µL, Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Immunohistochemistry (Paraffin): 1:100-1:1000, Western Blot: 1:1000
别名 :
2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; Enk; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; homeodomain transcription factor Nanog; NANOG; Nanog homeobox; Nanog homeodomain transcription factor; nanog homeodox protein
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公司信息
赛默飞世尔