产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A多克隆抗体
目录 :
PA1-16601
规格 :
100µL
价格 :
399.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
中国人仓鼠, 人类, 小鼠, 大鼠, , pigs , domestic rabbit
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 11
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 1:1000; 图 1b
Xiao N, Yang L, Yang Y, Liu L, Li J, Liu B, et al. Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance. Front Pharmacol. 2017;8:43 pubmed 出版商
  • 免疫印迹; 小鼠; 图 3
Zhao W, Li A, Feng X, Hou T, Liu K, Liu B, et al. Metformin and resveratrol ameliorate muscle insulin resistance through preventing lipolysis and inflammation in hypoxic adipose tissue. Cell Signal. 2016;28:1401-11 pubmed 出版商
Sivaraj K, Dharmalingam B, Mohanakrishnan V, Jeong H, Kato K, Schröder S, et al. YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells. elife. 2020;9: pubmed 出版商
Hsu H, Liao P, Cheng Y, Huang S, Wu C, Li C, et al. Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells. Int J Mol Sci. 2019;20: pubmed 出版商
Mira E, Carmona Rodríguez L, Pérez Villamil B, Casas J, Fernández Aceñero M, Martínez Rey D, et al. SOD3 improves the tumor response to chemotherapy by stabilizing endothelial HIF-2α. Nat Commun. 2018;9:575 pubmed 出版商
Meng Q, Qin Y, Deshpande M, Kashiwabuchi F, Rodrigues M, Lu Q, et al. Hypoxia-Inducible Factor-Dependent Expression of Angiopoietin-Like 4 by Conjunctival Epithelial Cells Promotes the Angiogenic Phenotype of Pterygia. Invest Ophthalmol Vis Sci. 2017;58:4514-4523 pubmed 出版商
Amofa D, Hulin A, Nakada Y, Sadek H, Yutzey K. Hypoxia promotes primitive glycosaminoglycan-rich extracellular matrix composition in developing heart valves. Am J Physiol Heart Circ Physiol. 2017;313:H1143-H1154 pubmed 出版商
Iguchi N, Malykhina A, Wilcox D. Inhibition of HIF Reduces Bladder Hypertrophy and Improves Bladder Function in Murine Model of Partial Bladder Outlet Obstruction. J Urol. 2016;195:1250-6 pubmed 出版商
Huang J, Liu L, Feng M, An S, Zhou M, Li Z, et al. Effect of CoClâ‚‚ on fracture repair in a rat model of bone fracture. Mol Med Rep. 2015;12:5951-6 pubmed 出版商
Xu X, Tan X, Tampe B, Sánchez E, Zeisberg M, Zeisberg E. Snail Is a Direct Target of Hypoxia-inducible Factor 1α (HIF1α) in Hypoxia-induced Endothelial to Mesenchymal Transition of Human Coronary Endothelial Cells. J Biol Chem. 2015;290:16653-64 pubmed 出版商
Guven M, Sehitoglu M, Yüksel Y, Tokmak M, Aras A, Akman T, et al. The Neuroprotective Effect of Coumaric Acid on Spinal Cord Ischemia/Reperfusion Injury in Rats. Inflammation. 2015;38:1986-95 pubmed 出版商
图像
图像 1 :
赛默飞世尔 PA1-16601 图像 1
Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human placenta villi tissue section using PA1-16601.
图像 2 :
赛默飞世尔 PA1-16601 图像 2
Detection of HIF-1 Alpha (green) in RCC4 cells using PA1-16601. Nuclei (Blue) were counterstained using Hoechst 33258.
图像 3 :
赛默飞世尔 PA1-16601 图像 3
Western blot analysis of HIF-1 alpha was performed by loading 50 ug of Cos7 cell lysates under hypoxic (Lane 1: CoCl2 treated) or untreated (Lane 2) conditions onto a gel and then transferring blotted to membrane. Blot was probed with a rabbit polyclonal antibody recognizing HIF-1 alpha (Product # PA1-16601) and chemiluminescent deterction performed using ECL.
产品信息
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A多克隆抗体
目录# :
PA1-16601
规格 :
100µL
价格(美元) :
399.00美元
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
反应物种 :
Canine, Fish, Hamster, Human, Mouse, Non-human primate, Porcine, Rabbit, Rat
应用 :
ChIP assay: 1:10-1:500, Flow Cytometry: 1:10-1:1000, Immunocytochemistry: 1:100, Immunofluorescence: 1:100, Immunohistochemistry (Frozen): 5-10 µg/mL, Immunohistochemistry (Paraffin): 5-10 µg/mL, Western Blot: 1:500-1:2000
物种 :
Canine, Fish, Hamster, Human, Mouse, Non-human primate, Porcine, Rabbit, Rat
抗体亚型 :
IgG
储存 :
4° C, 不冻结
描述 :
HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
免疫原 :
A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein.
格式 :
液体
应用w/稀释 :
ChIP assay: 1:10-1:500, Flow Cytometry: 1:10-1:1000, Immunocytochemistry: 1:100, Immunofluorescence: 1:100, Immunohistochemistry (Frozen): 5-10 µg/mL, Immunohistochemistry (Paraffin): 5-10 µg/mL, Western Blot: 1:500-1:2000
别名 :
AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; HIF-1A; hif1a.L; HIF1alpha; hif-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; Hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor-1 alpha; hypoxia-inducible factor1alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国