产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A多克隆抗体
目录 :
PA1-16601
规格 :
100µL
价格 :
美国381.00
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
中国人仓鼠, 人类, 小鼠, 大鼠,
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 11
出版应用/物种/样品/稀释参考文献
  • 免疫印迹; 小鼠; 1:1000; 图 1b
Xiao N, Yang L, Yang Y, Liu L, Li J, Liu B, et al. Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance. Front Pharmacol. 2017;8:43 pubmed 出版商
  • 免疫印迹; 小鼠; 图 3
Zhao W, Li A, Feng X, Hou T, Liu K, Liu B, et al. Metformin and resveratrol ameliorate muscle insulin resistance through preventing lipolysis and inflammation in hypoxic adipose tissue. Cell Signal. 2016;28:1401-11 pubmed 出版商
  • 免疫印迹; 大鼠; 图 4
Huang J, Liu L, Feng M, An S, Zhou M, Li Z, et al. Effect of CoClâ‚‚ on fracture repair in a rat model of bone fracture. Mol Med Rep. 2015;12:5951-6 pubmed 出版商
  • 免疫组化-石蜡切片; 大鼠; 1:100; 图 5
Guven M, Sehitoglu M, Yüksel Y, Tokmak M, Aras A, Akman T, et al. The Neuroprotective Effect of Coumaric Acid on Spinal Cord Ischemia/Reperfusion Injury in Rats. Inflammation. 2015;38:1986-95 pubmed 出版商
  • 免疫组化; 人类; 1:500
Pate K, Stringari C, Sprowl Tanio S, Wang K, Teslaa T, Hoverter N, et al. Wnt signaling directs a metabolic program of glycolysis and angiogenesis in colon cancer. EMBO J. 2014;33:1454-73 pubmed 出版商
  • 免疫印迹; 兔
Tsuchida S, Arai Y, Takahashi K, Kishida T, Terauchi R, Honjo K, et al. HIF-1?-induced HSP70 regulates anabolic responses in articular chondrocytes under hypoxic conditions. J Orthop Res. 2014;32:975-80 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000
Pereira S, Grãos M, Rodrigues A, Anjo S, Carvalho R, Oliveira P, et al. Inhibition of mitochondrial complex III blocks neuronal differentiation and maintains embryonic stem cell pluripotency. PLoS ONE. 2013;8:e82095 pubmed 出版商
  • 免疫印迹; 大鼠; 1:10000
Reddy V, Raghu G, Reddy S, Pasupulati A, Suryanarayana P, Reddy G. Response of small heat shock proteins in diabetic rat retina. Invest Ophthalmol Vis Sci. 2013;54:7674-82 pubmed 出版商
  • 免疫印迹; 小鼠; 0.25 ug/ml
Ishizuka T, Hinata T, Watanabe Y. Superoxide induced by a high-glucose concentration attenuates production of angiogenic growth factors in hypoxic mouse mesenchymal stem cells. J Endocrinol. 2011;208:147-59 pubmed 出版商
Meng Q, Qin Y, Deshpande M, Kashiwabuchi F, Rodrigues M, Lu Q, et al. Hypoxia-Inducible Factor-Dependent Expression of Angiopoietin-Like 4 by Conjunctival Epithelial Cells Promotes the Angiogenic Phenotype of Pterygia. Invest Ophthalmol Vis Sci. 2017;58:4514-4523 pubmed 出版商
Xu X, Tan X, Tampe B, Sánchez E, Zeisberg M, Zeisberg E. Snail Is a Direct Target of Hypoxia-inducible Factor 1α (HIF1α) in Hypoxia-induced Endothelial to Mesenchymal Transition of Human Coronary Endothelial Cells. J Biol Chem. 2015;290:16653-64 pubmed 出版商
图像
图像 1 :
赛默飞世尔 PA1-16601 图像 1
Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human placenta villi tissue section using PA1-16601.
图像 2 :
赛默飞世尔 PA1-16601 图像 2
Detection of HIF-1 Alpha (green) in RCC4 cells using PA1-16601. Nuclei (Blue) were counterstained using Hoechst 33258.
图像 3 :
赛默飞世尔 PA1-16601 图像 3
Western blot analysis of HIF-1 alpha was performed by loading 50 ug of Cos7 cell lysates under hypoxic (Lane 1: CoCl2 treated) or untreated (Lane 2) conditions onto a gel and then transferring blotted to membrane. Blot was probed with a rabbit polyclonal antibody recognizing HIF-1 alpha (Product # PA1-16601) and chemiluminescent deterction performed using ECL.
产品信息
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A多克隆抗体
目录# :
PA1-16601
规格 :
100µL
价格 :
美国381.00
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
反应物种 :
Hamster, Human, Mouse, Non-human primate, Rabbit, Rat
应用 :
ChIP assay: Assay-Dependent, Flow Cytometry: Assay-Dependent, Immunocytochemistry: 1:100, Immunofluorescence: 1:100, Immunohistochemistry (Frozen): 5-10 µg/mL, Immunohistochemistry (Paraffin): 5-10 µg/mL, Western Blot: 1:500-1:2000
物种 :
Hamster, Human, Mouse, Non-human primate, Rabbit, Rat
抗体亚型 :
IgG
储存 :
4° C, 不冻结
描述 :
HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
免疫原 :
A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein.
格式 :
Liquid
应用w/稀释 :
ChIP assay: Assay-Dependent, Flow Cytometry: Assay-Dependent, Immunocytochemistry: 1:100, Immunofluorescence: 1:100, Immunohistochemistry (Frozen): 5-10 µg/mL, Immunohistochemistry (Paraffin): 5-10 µg/mL, Western Blot: 1:500-1:2000
别名 :
Hypoxia Inducible Factor 1 alpha, ARNT interacting protein, ARNT2, MOP1, PASD8
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
www.thermo.com.cn
1084193588
公司总部: 美国