产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
LC3A/LC3B多克隆抗体
目录 :
PA1-16931
规格 :
100µL
价格 :
美国390
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠, , 斑马鱼
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 5
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 图 4a
Zou P, Liu L, Zheng L, Payne K, Manjili M, Idowu M, et al. Coordinated Upregulation of Mitochondrial Biogenesis and Autophagy in Breast Cancer Cells: The Role of Dynamin Related Protein-1 and Implication for Breast Cancer Treatment. Oxid Med Cell Longev. 2016;2016:4085727 pubmed
  • 免疫印迹; 大鼠; 图 8
Shruthi K, Reddy S, Reddy P, Shivalingam P, Harishankar N, Reddy G. Amelioration of neuronal cell death in a spontaneous obese rat model by dietary restriction through modulation of ubiquitin proteasome system. J Nutr Biochem. 2016;33:73-81 pubmed 出版商
  • 免疫印迹; 人类; 图 s2a
Pereira D, Simões A, Gomes S, Castro R, Carvalho T, Rodrigues C, et al. MEK5/ERK5 signaling inhibition increases colon cancer cell sensitivity to 5-fluorouracil through a p53-dependent mechanism. Oncotarget. 2016;7:34322-40 pubmed 出版商
Ferreira J, Soares A, Ramalho J, Pereira P, Girao H. K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy. Sci Rep. 2015;5:10210 pubmed 出版商
Morgado A, Xavier J, Dionísio P, Ribeiro M, Dias R, Sebastião A, et al. MicroRNA-34a Modulates Neural Stem Cell Differentiation by Regulating Expression of Synaptic and Autophagic Proteins. Mol Neurobiol. 2015;51:1168-83 pubmed 出版商
图像
图像 1 :
赛默飞世尔 PA1-16931 图像 1
Immunohistochemistry staining of human brain, cerebral cortex, cell crocesses in gray matter.
图像 2 :
赛默飞世尔 PA1-16931 图像 2
Western blot of mouse ES cell lysate showing specific labeling of the LC3-1 and LC3-2 proteins.
产品信息
产品类型 :
抗体
产品名称 :
LC3A/LC3B多克隆抗体
目录# :
PA1-16931
规格 :
100µL
价格 :
美国390
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
反应物种 :
狗, 鱼类, 人类, 小鼠, 植物, 大鼠, 斑马鱼
应用 :
ELISA: Assay-Dependent, Flow Cytometry: Assay-Dependent, Immunocytochemistry: 1:100-1:300, Immunofluorescence: 1:100-1:300, Immunohistochemistry (Frozen): Assay-Dependent, Immunohistochemistry (Paraffin): 1:200-1:400, Immunoprecipitation: 20 µg/500 µg of lysate, Western Blot: 2 µg/mL
物种 :
狗, 鱼类, 人类, 小鼠, 植物, 大鼠, 斑马鱼
抗体亚型 :
IgG
储存 :
-20°C or -80°C if preferred
描述 :
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
免疫原 :
Synthetic peptide corresponding to a region between residues 25-121 of human LC3 protein.
格式 :
液体
应用w/稀释 :
ELISA: Assay-Dependent, Flow Cytometry: Assay-Dependent, Immunocytochemistry: 1:100-1:300, Immunofluorescence: 1:100-1:300, Immunohistochemistry (Frozen): Assay-Dependent, Immunohistochemistry (Paraffin): 1:200-1:400, Immunoprecipitation: 20 µg/500 µg of lysate, Western Blot: 2 µg/mL
别名 :
1010001C15Rik; 1010001H21Rik; 4922501H04Rik; Atg8; ATG8E; ATG8F; Autophagy-related protein LC3 A; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 A; Autophagy-related ubiquitin-like modifier LC3 B; LC3; LC3A; LC3b; MAP1 light chain 3-like protein 1; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B light chain 3 B; Map1alc3; MAP1BLC3; Map1lc3; MAP1LC3A; MAP1LC3B; MAP1LC3B-a; microtubule associated protein 1 light chain 3 alpha; microtubule associated protein 1 light chain 3 beta; microtubule-associated protein 1 light chain 3; Microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1-light chain 3A; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; Microtubule-associated proteins 1A/1B light chain 3B; Mpl3; wu:fb60g11; zbs559; zgc:56434; zgc:77094
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国