产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
组蛋白H2A.X多克隆抗体
目录 :
PA1-41004
规格 :
100微克
价格 :
美国496.00
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 2
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图像
图像 1 :
产品信息
产品类型 :
抗体
产品名称 :
组蛋白H2A.X多克隆抗体
目录# :
PA1-41004
规格 :
100微克
价格 :
美国496.00
克隆性 :
多克隆
纯度 :
蛋白质G
宿主 :
兔
反应物种 :
Human, Mouse, Ovine
应用 :
ChIP Assay: 1.5 µg per million cells, ELISA: 1:2,000, Flow Cytometry: Assay-Dependent, Immunohistochemistry (Paraffin): 5 µg/mL, Western Blot: 2 µg/mL
物种 :
Human, Mouse, Ovine
抗体亚型 :
IgG
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.
免疫原 :
Mixture of synthetic peptides containing residues 1-15 and 81-96 of Human Histone H2A
格式 :
液体
应用w/稀释 :
ChIP Assay: 1.5 µg per million cells, ELISA: 1:2,000, Flow Cytometry: Assay-Dependent, Immunohistochemistry (Paraffin): 5 µg/mL, Western Blot: 2 µg/mL
别名 :
AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2A.X; H2A.X variant histone; H2A/X; h2afx; H2ax; H2AX histone; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329
更多信息或购买 :
公司信息
赛默飞世尔