产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
MBD4多克隆抗体
目录 :
PA1-41106
规格 :
100微克
价格 :
美国446.00
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹
更多信息或购买 :
图像
图像 1 :
赛默飞世尔 PA1-41106 图像 1
产品信息
产品类型 :
抗体
产品名称 :
MBD4多克隆抗体
目录# :
PA1-41106
规格 :
100微克
价格 :
美国446.00
克隆性 :
多克隆
纯度 :
蛋白质G
宿主 :
反应物种 :
人类, 小鼠
应用 :
ChIP Assay: Assay-Dependent, Western Blot: 2-4 µg/mL
物种 :
人类, 小鼠
抗体亚型 :
IgG
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
免疫原 :
A mixture of synthetic peptides corresponding to amino acids 268-300 and 337-374 of human MBD4.
格式 :
液体
应用w/稀释 :
ChIP Assay: Assay-Dependent, Western Blot: 2-4 µg/mL
别名 :
3,N(4)-ethenocytosine glycosylase; G/5-fluorouracil mismatch glycosylase with biphasic kinetics; G/T mismatch glycosylase; G/U mismatch glycosylase; Mbd4; MED1; methyl-CpG binding domain 4 DNA glycosylase; methyl-CpG binding domain 4, DNA glycosylase; methyl-CpG binding domain protein 4; methyl-CpG-binding domain protein 4; Methyl-CpG-binding endonuclease 1; methyl-CpG-binding protein MBD4; Mismatch-specific DNA N-glycosylase; putative methyl-CpG binding protein
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国