产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A多克隆抗体
目录 :
PA3-16521
规格 :
100µL
价格 :
美国381.00
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
Domestic guinea pig, 人类, 小鼠, 大鼠, , common platanna
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 3
出版应用/物种/样品/稀释参考文献
  • 免疫印迹; 小鼠; 1:500; 图 9a
Djamali A, Wilson N, Sadowski E, Zha W, Niles D, Hafez O, et al. Nox2 and Cyclosporine-Induced Renal Hypoxia. Transplantation. 2016;100:1198-210 pubmed 出版商
  • 免疫细胞化学; 人类; 1:200
  • 免疫印迹; 人类; 1:1000
Zuloaga K, Gonzales R. Dihydrotestosterone attenuates hypoxia inducible factor-1? and cyclooxygenase-2 in cerebral arteries during hypoxia or hypoxia with glucose deprivation. Am J Physiol Heart Circ Physiol. 2011;301:H1882-90 pubmed 出版商
  • 免疫沉淀; 人类
Luo D, Wang J, Li J, Post M. Mouse snail is a target gene for HIF. Mol Cancer Res. 2011;9:234-45 pubmed 出版商
图像
图像 1 :
赛默飞世尔 PA3-16521 图像 1
图像 2 :
赛默飞世尔 PA3-16521 图像 2
Immunohistochemical staining of human kidney, renal tubular epithelium in Cortex using PA3-16521.
图像 3 :
赛默飞世尔 PA3-16521 图像 3
Western Blot analysis of PA3-16521, anti-HIF-1 alpha, on normoxic and hypoxic nuclear rat cell lysates.
产品信息
产品类型 :
抗体
产品名称 :
缺氧诱导因子1A多克隆抗体
目录# :
PA3-16521
规格 :
100µL
价格 :
美国381.00
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
反应物种 :
Canine, Guinea Pig, Human, Mouse, Non-human primate, Rat, Xenopus laevis
应用 :
ChIP assay: 25 µL/1.5x10^7 cells, Gel Shift: 1:1-1:100, Immunocytochemistry: 1:10-1:500, Immunofluorescence: 1:10-1:500, Immunohistochemistry (Frozen): 1:10-1:500, Immunohistochemistry (Paraffin): 1:100, Immunoprecipitation: 1:1000, Western Blot: 1:500-1:1000
物种 :
Canine, Guinea Pig, Human, Mouse, Non-human primate, Rat, Xenopus laevis
抗体亚型 :
IgG
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
免疫原 :
融合蛋白含氨基酸432-528人的HIF-1alpha
格式 :
Liquid
应用w/稀释 :
ChIP assay: 25 µL/1.5x10^7 cells, Gel Shift: 1:1-1:100, Immunocytochemistry: 1:10-1:500, Immunofluorescence: 1:10-1:500, Immunohistochemistry (Frozen): 1:10-1:500, Immunohistochemistry (Paraffin): 1:100, Immunoprecipitation: 1:1000, Western Blot: 1:500-1:1000
别名 :
Hypoxia Inducible Factor 1 alpha, ARNT interacting protein, ARNT2, MOP1, PASD8
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
www.thermo.com.cn
1084193588
公司总部: 美国