产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
钙网蛋白多克隆抗体
目录 :
PA3-900
规格 :
100微升
价格 :
美国455.00
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
African green monkey, 人类, 小鼠, 大鼠, , domestic rabbit
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 流式细胞仪
更多信息或购买 :
文章摘录数: 65
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 图 3g
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht M, et al. Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum. Neuron. 2021;109:299-313.e9 pubmed 出版商
  • 免疫细胞化学; 人类; 1:300; 图 5a
Wang G, Xu J, Zhao J, Yin W, Liu D, Chen W, et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice. Nat Commun. 2020;11:220 pubmed 出版商
  • 免疫印迹; 人类; 图 4c
Wang W, Wang J, Lin W, Kao C, Hung M, Teng Y, et al. Progerin in muscle leads to thermogenic and metabolic defects via impaired calcium homeostasis. Aging Cell. 2020;19:e13090 pubmed 出版商
  • 免疫细胞化学; African green monkey; 图 5e
Tiwarekar V, Fehrholz M, Schneider Schaulies J. KDELR2 Competes with Measles Virus Envelope Proteins for Cellular Chaperones Reducing Their Chaperone-Mediated Cell Surface Transport. Viruses. 2019;11: pubmed 出版商
  • 流式细胞仪; 人类; 图 10b
Granato M, Rizzello C, Gilardini Montani M, Cuomo L, Vitillo M, Santarelli R, et al. Quercetin induces apoptosis and autophagy in primary effusion lymphoma cells by inhibiting PI3K/AKT/mTOR and STAT3 signaling pathways. J Nutr Biochem. 2017;41:124-136 pubmed 出版商
  • 免疫细胞化学; 人类; 图 3a
Ishimura R, Obata M, Kageyama S, Daniel J, Tanaka K, Komatsu M. A novel approach to assess the ubiquitin-fold modifier 1-system in cells. FEBS Lett. 2017;591:196-204 pubmed 出版商
  • 免疫印迹; 人类; 图 4b
Greenwood E, Matheson N, Wals K, van den Boomen D, Antrobus R, Williamson J, et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. elife. 2016;5: pubmed 出版商
  • 免疫细胞化学; 人类; 1:100; 图 1b
Greotti E, Wong A, Pozzan T, Pendin D, Pizzo P. Characterization of the ER-Targeted Low Affinity Ca(2+) Probe D4ER. Sensors (Basel). 2016;16: pubmed 出版商
  • 免疫印迹; 人类; 图 1
Liu C, LeClair P, Monajemi M, Sly L, Reid G, Lim C. α-Integrin expression and function modulates presentation of cell surface calreticulin. Cell Death Dis. 2016;7:e2268 pubmed 出版商
  • 免疫印迹; 小鼠; 1:1000; 图 3
Mosca B, Eckhardt J, Bergamelli L, Treves S, Bongianino R, De Negri M, et al. Role of the JP45-Calsequestrin Complex on Calcium Entry in Slow Twitch Skeletal Muscles. J Biol Chem. 2016;291:14555-65 pubmed 出版商
  • 免疫细胞化学; 小鼠; 图 2a
Shih Y, Hsueh Y. VCP and ATL1 regulate endoplasmic reticulum and protein synthesis for dendritic spine formation. Nat Commun. 2016;7:11020 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 2
Tagliamonte M, Petrizzo A, Napolitano M, Luciano A, Rea D, Barbieri A, et al. A novel multi-drug metronomic chemotherapy significantly delays tumor growth in mice. J Transl Med. 2016;14:58 pubmed 出版商
  • 免疫组化; 小鼠; 1:1000; 图 8
Ruegsegger C, Stucki D, Steiner S, Angliker N, Radecke J, Keller E, et al. Impaired mTORC1-Dependent Expression of Homer-3 Influences SCA1 Pathophysiology. Neuron. 2016;89:129-46 pubmed 出版商
Zhang R, Neighbors J, Schell T, Hohl R. Schweinfurthin induces ICD without ER stress and caspase activation. Oncoimmunology. 2022;11:2104551 pubmed 出版商
Haney S, Varney M, Williams J, Smith L, Talmon G, Holstein S. Geranylgeranyl diphosphate synthase inhibitor and proteasome inhibitor combination therapy in multiple myeloma. Exp Hematol Oncol. 2022;11:5 pubmed 出版商
Abdullah T, Whatmore J, Bremer E, Slibinskas R, Michalak M, Eggleton P. Endoplasmic reticulum stress-induced release and binding of calreticulin from human ovarian cancer cells. Cancer Immunol Immunother. 2022;71:1655-1669 pubmed 出版商
Cali T, Brini M. Quantification of organelle contact sites by split-GFP-based contact site sensors (SPLICS) in living cells. Nat Protoc. 2021;16:5287-5308 pubmed 出版商
Bai X, Zhou Y, Lin Q, Huang C, Zhang S, Carlson R, et al. Bio-nanoparticle based therapeutic vaccine induces immunogenic response against triple negative breast cancer. Am J Cancer Res. 2021;11:4141-4174 pubmed
Sampieri L, Funes Chab xe1 n M, Di Giusto P, Roz xe9 s Salvador V, Alvarez C. CREB3L2 Modulates Nerve Growth Factor-Induced Cell Differentiation. Front Mol Neurosci. 2021;14:650338 pubmed 出版商
Tukaramrao D, Malla S, Saraiya S, Hanely R, Ray A, Kumari S, et al. A Novel Thienopyrimidine Analog, TPH104, Mediates Immunogenic Cell Death in Triple-Negative Breast Cancer Cells. Cancers (Basel). 2021;13: pubmed 出版商
Venkatesan A, Geng J, Kandarpa M, Wijeyesakere S, Bhide A, Talpaz M, et al. Mechanism of mutant calreticulin-mediated activation of the thrombopoietin receptor in cancers. J Cell Biol. 2021;220: pubmed 出版商
Pavlou M, Sch xf6 n C, Occelli L, Rossi A, Meumann N, Boyd R, et al. Novel AAV capsids for intravitreal gene therapy of photoreceptor disorders. EMBO Mol Med. 2021;13:e13392 pubmed 出版商
Theodoridis P, Bokros M, Marijan D, Balukoff N, Wang D, Kirk C, et al. Local translation in nuclear condensate amyloid bodies. Proc Natl Acad Sci U S A. 2021;118: pubmed 出版商
Mączyńska J, Da Pieve C, Burley T, Raes F, Shah A, Saczko J, et al. Immunomodulatory activity of IR700-labelled affibody targeting HER2. Cell Death Dis. 2020;11:886 pubmed 出版商
Mohan H, Yang B, Dean N, Raghavan M. Calreticulin enhances the secretory trafficking of a misfolded α-1-antitrypsin. J Biol Chem. 2020;295:16754-16772 pubmed 出版商
Mei K, Liao Y, Jiang J, Chiang M, Khazaieli M, Liu X, et al. Liposomal Delivery of Mitoxantrone and a Cholesteryl Indoximod Prodrug Provides Effective Chemo-immunotherapy in Multiple Solid Tumors. ACS Nano. 2020;14:13343-13366 pubmed 出版商
Di Grazia A, Laudisi F, Di Fusco D, Franzè E, Ortenzi A, Monteleone I, et al. Rafoxanide Induces Immunogenic Death of Colorectal Cancer Cells. Cancers (Basel). 2020;12: pubmed 出版商
Mozzi A, Biolatti M, Cagliani R, Forni D, Dell Oste V, Pontremoli C, et al. Past and ongoing adaptation of human cytomegalovirus to its host. PLoS Pathog. 2020;16:e1008476 pubmed 出版商
Furlan S, Campione M, Murgia M, Mosole S, Argenton F, Volpe P, et al. Calsequestrins New Calcium Store Markers of Adult Zebrafish Cerebellum and Optic Tectum. Front Neuroanat. 2020;14:15 pubmed 出版商
Kopecka J, Godel M, Dei S, Giampietro R, Belisario D, Akman M, et al. Insights into P-Glycoprotein Inhibitors: New Inducers of Immunogenic Cell Death. Cells. 2020;9: pubmed 出版商
Biwer L, Askew Page H, Hong K, Milstein J, Johnstone S, Macal E, et al. Endothelial calreticulin deletion impairs endothelial function in aged mice. Am J Physiol Heart Circ Physiol. 2020;318:H1041-H1048 pubmed 出版商
Suzuki E, Ogawa N, Takeda T, Nishito Y, Tanaka Y, Fujiwara T, et al. Detailed analyses of the crucial functions of Zn transporter proteins in alkaline phosphatase activation. J Biol Chem. 2020;295:5669-5684 pubmed 出版商
Seyedin S, Hasibuzzaman M, Pham V, Petronek M, Callaghan C, Kalen A, et al. Combination Therapy with Radiation and PARP Inhibition Enhances Responsiveness to Anti-PD-1 Therapy in Colorectal Tumor Models. Int J Radiat Oncol Biol Phys. 2020;108:81-92 pubmed 出版商
Ma J, Ramachandran M, Jin C, Quijano Rubio C, Martikainen M, Yu D, et al. Characterization of virus-mediated immunogenic cancer cell death and the consequences for oncolytic virus-based immunotherapy of cancer. Cell Death Dis. 2020;11:48 pubmed 出版商
Benfield C, MacKenzie F, Ritzefeld M, Mazzon M, Weston S, Tate E, et al. Bat IFITM3 restriction depends on S-palmitoylation and a polymorphic site within the CD225 domain. Life Sci Alliance. 2020;3: pubmed 出版商
Tiscione S, Vivas O, Ginsburg K, Bers D, Ory D, Santana L, et al. Disease-associated mutations in Niemann-Pick type C1 alter ER calcium signaling and neuronal plasticity. J Cell Biol. 2019;218:4141-4156 pubmed 出版商
Liu C, Leclair P, Pedari F, Vieira H, Monajemi M, Sly L, et al. Integrins and ERp57 Coordinate to Regulate Cell Surface Calreticulin in Immunogenic Cell Death. Front Oncol. 2019;9:411 pubmed 出版商
Anwar T, Liu X, Suntio T, Marjamäki A, Biazik J, Chan E, et al. ER-Targeted Beclin 1 Supports Autophagosome Biogenesis in the Absence of ULK1 and ULK2 Kinases. Cells. 2019;8: pubmed 出版商
Rossi A, Dupaty L, Aillot L, Zhang L, Gallien C, Hallek M, et al. Vector uncoating limits adeno-associated viral vector-mediated transduction of human dendritic cells and vector immunogenicity. Sci Rep. 2019;9:3631 pubmed 出版商
Pandya U, Egbuta C, Abdullah Norman T, Chiang C, Wiersma V, Panchal R, et al. The Biophysical Interaction of the Danger-Associated Molecular Pattern (DAMP) Calreticulin with the Pattern-Associated Molecular Pattern (PAMP) Lipopolysaccharide. Int J Mol Sci. 2019;20: pubmed 出版商
Champion L, Pawar S, Luithle N, Ungricht R, Kutay U. Dissociation of membrane-chromatin contacts is required for proper chromosome segregation in mitosis. Mol Biol Cell. 2019;30:427-440 pubmed 出版商
Chorro L, Suzuki M, Chin S, Williams T, Snapp E, Odagiu L, et al. Interleukin 2 modulates thymic-derived regulatory T cell epigenetic landscape. Nat Commun. 2018;9:5368 pubmed 出版商
Menzies S, Volkmar N, van den Boomen D, Timms R, Dickson A, Nathan J, et al. The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. elife. 2018;7: pubmed 出版商
Salaroglio I, Gazzano E, Abdullrahman A, Mungo E, Castella B, Abd Elrahman G, et al. Increasing intratumor C/EBP-β LIP and nitric oxide levels overcome resistance to doxorubicin in triple negative breast cancer. J Exp Clin Cancer Res. 2018;37:286 pubmed 出版商
Takeda T, Miyazaki S, Kobayashi M, Nishino K, Goto T, Matsunaga M, et al. Zinc deficiency causes delayed ATP clearance and adenosine generation in rats and cell culture models. Commun Biol. 2018;1:113 pubmed 出版商
Plaud C, Joshi V, Kajevu N, Poüs C, Curmi P, Burgo A. Functional differences of short and long isoforms of spastin harboring missense mutation. Dis Model Mech. 2018;11: pubmed 出版商
Li L, Jiao X, D Atri I, Ono F, Nelson R, Chan C, et al. Mutation in the intracellular chloride channel CLCC1 associated with autosomal recessive retinitis pigmentosa. PLoS Genet. 2018;14:e1007504 pubmed 出版商
Vigneron N, Ferrari V, van den Eynde B, Cresswell P, Leonhardt R. Cytosolic Processing Governs TAP-Independent Presentation of a Critical Melanoma Antigen. J Immunol. 2018;201:1875-1888 pubmed 出版商
Hangen E, Cordelières F, Petersen J, Choquet D, Coussen F. Neuronal Activity and Intracellular Calcium Levels Regulate Intracellular Transport of Newly Synthesized AMPAR. Cell Rep. 2018;24:1001-1012.e3 pubmed 出版商
Arshad N, Cresswell P. Tumor-associated calreticulin variants functionally compromise the peptide loading complex and impair its recruitment of MHC-I. J Biol Chem. 2018;293:9555-9569 pubmed 出版商
Borrelli C, Ricci B, Vulpis E, Fionda C, Ricciardi M, Petrucci M, et al. Drug-Induced Senescent Multiple Myeloma Cells Elicit NK Cell Proliferation by Direct or Exosome-Mediated IL15 Trans-Presentation. Cancer Immunol Res. 2018;6:860-869 pubmed 出版商
Xing X, Mroß C, Hao L, Munck M, Herzog A, Mohr C, et al. Nesprin-2 Interacts with Condensin Component SMC2. Int J Cell Biol. 2017;2017:8607532 pubmed 出版商
Cieri D, Vicario M, Giacomello M, Vallese F, Filadi R, Wagner T, et al. SPLICS: a split green fluorescent protein-based contact site sensor for narrow and wide heterotypic organelle juxtaposition. Cell Death Differ. 2018;25:1131-1145 pubmed 出版商
Wang Y, Xie J, Liu Z, Fu H, Huo Q, Gu Y, et al. Association of calreticulin expression with disease activity and organ damage in systemic lupus erythematosus patients. Exp Ther Med. 2017;13:2577-2583 pubmed 出版商
Doghman Bouguerra M, Granatiero V, Sbiera S, Sbiera I, Lacas Gervais S, Brau F, et al. FATE1 antagonizes calcium- and drug-induced apoptosis by uncoupling ER and mitochondria. EMBO Rep. 2016;17:1264-80 pubmed 出版商
Wijeyesakere S, Gagnon J, Arora K, Brooks C, Raghavan M. Regulation of calreticulin-major histocompatibility complex (MHC) class I interactions by ATP. Proc Natl Acad Sci U S A. 2015;112:E5608-17 pubmed 出版商
Granato M, Gilardini Montani M, Filardi M, Faggioni A, Cirone M. Capsaicin triggers immunogenic PEL cell death, stimulates DCs and reverts PEL-induced immune suppression. Oncotarget. 2015;6:29543-54 pubmed 出版商
Williamson C, Wong D, Bozidis P, Zhang A, COLBERG POLEY A. Isolation of Endoplasmic Reticulum, Mitochondria, and Mitochondria-Associated Membrane and Detergent Resistant Membrane Fractions from Transfected Cells and from Human Cytomegalovirus-Infected Primary Fibroblasts. Curr Protoc Cell Biol. 2015;68:3.27.1-33 pubmed 出版商
Parkinson M, Piper S, Bright N, Evans J, Boname J, Bowers K, et al. A non-canonical ESCRT pathway, including histidine domain phosphotyrosine phosphatase (HD-PTP), is used for down-regulation of virally ubiquitinated MHC class I. Biochem J. 2015;471:79-88 pubmed 出版商
Matsuno H, Ohi K, Hashimoto R, Yamamori H, Yasuda Y, Fujimoto M, et al. A naturally occurring null variant of the NMDA type glutamate receptor NR3B subunit is a risk factor of schizophrenia. PLoS ONE. 2015;10:e0116319 pubmed 出版商
Tafaleng E, Chakraborty S, Han B, Hale P, Wu W, Soto Gutierrez A, et al. Induced pluripotent stem cells model personalized variations in liver disease resulting from α1-antitrypsin deficiency. Hepatology. 2015;62:147-57 pubmed 出版商
Filézac de L Etang A, Maharjan N, Cordeiro Braña M, Ruegsegger C, Rehmann R, Goswami A, et al. Marinesco-Sjögren syndrome protein SIL1 regulates motor neuron subtype-selective ER stress in ALS. Nat Neurosci. 2015;18:227-38 pubmed 出版商
Turpin J, Journo C, Ko N, Sinet F, Carpentier A, Galioot A, et al. Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic viruses. J Virol. 2015;89:931-51 pubmed 出版商
Bernard Marissal N, Sunyach C, Marissal T, Raoul C, Pettmann B. Calreticulin levels determine onset of early muscle denervation by fast motoneurons of ALS model mice. Neurobiol Dis. 2015;73:130-6 pubmed 出版商
Pearse B, Gabriel L, Wang N, Hebert D. A cell-based reglucosylation assay demonstrates the role of GT1 in the quality control of a maturing glycoprotein. J Cell Biol. 2008;181:309-20 pubmed 出版商
图像
图像 1 :
赛默飞世尔 PA3-900 图像 1
Immunofluorescent analysis of Phalloidin (orange) and Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
图像 2 :
赛默飞世尔 PA3-900 图像 2
Immunofluorescent analysis of Calreticulin (green) U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS 0.1% triton-X (Product #37525) for 30 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing Calreticulin (Product # PA3-900) at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (Product #35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin filaments (red) were stained with DyLight 554-Phalloidin (Product# 21834) at a dilution of 1:300 (i.e. 1unit/mL final concentration) in PBS and incubated for 30 min including. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249) at a dilution of 1µg/mL. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
图像 3 :
赛默飞世尔 PA3-900 图像 3
Immunofluorescent analysis of Phalloidin (green) and Calreticulin (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 633 goat anti-rabbit IgG secondary antibody (Product # 35562) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
产品信息
产品类型 :
抗体
产品名称 :
钙网蛋白多克隆抗体
目录# :
PA3-900
规格 :
100微升
价格 :
美国455.00
克隆性 :
多克隆
宿主 :
反应物种 :
Canine, Human, Mouse, Non-human primate, Rabbit, Rat
应用 :
Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:50 - 1:200, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000
物种 :
Canine, Human, Mouse, Non-human primate, Rabbit, Rat
抗体亚型 :
IgG
储存 :
-20° C, 避免反复冷冻/解冻
描述 :
Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.
免疫原 :
重组, 人类钙网蛋白蛋白
格式 :
液体
应用w/稀释 :
Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:50 - 1:200, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000
别名 :
Autoantigen RO; CABP3; CALBP; calcium-binding protein 3; CALR; calrectulin; calregulin; Calreticulin; calreticulin precursor; cC1qR; CRP55; CRT; CRTC; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; HEL-S-99n; I79_008346; RO; Ro/SS-A Antigen; Sicca syndrome antigen A (autoantigen Ro; calreticulin); SSA
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国