产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
磷酸化组蛋白H3(Ser10)多克隆抗体
目录 :
PA5-17869
规格 :
100微升
价格 :
美国445.00
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
抗原修饰 :
磷酸化
反应物种 :
人类, 小鼠, 大鼠, fruit fly , 非洲爪蛙
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 11
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 图 1
  • 免疫印迹; 人类; 图 1
Wang Q, Xue L, Zhang X, Bu S, Zhu X, Lai D. Autophagy protects ovarian cancer-associated fibroblasts against oxidative stress. Cell Cycle. 2016;15:1376-85 pubmed 出版商
Pizzo L, Lasser M, Yusuff T, Jensen M, Ingraham P, Huber E, et al. Functional assessment of the "two-hit" model for neurodevelopmental defects in Drosophila and X. laevis. PLoS Genet. 2021;17:e1009112 pubmed 出版商
Sun Y, Wang Z, Na L, Dong D, Wang W, Zhao C. FZD5 contributes to TNBC proliferation, DNA damage repair and stemness. Cell Death Dis. 2020;11:1060 pubmed 出版商
Lan C, Cao N, Chen C, Qu S, Fan C, Luo H, et al. Progesterone, via yes-associated protein, promotes cardiomyocyte proliferation and cardiac repair. Cell Prolif. 2020;53:e12910 pubmed 出版商
Hou L, Fu W, Liu Y, Wang Q, Wang L, Huang Y. Agrin Promotes Limbal Stem Cell Proliferation and Corneal Wound Healing Through Hippo-Yap Signaling Pathway. Invest Ophthalmol Vis Sci. 2020;61:7 pubmed 出版商
Xu R, Brawner A, Li S, Liu J, Kim H, Xue H, et al. OLIG2 Drives Abnormal Neurodevelopmental Phenotypes in Human iPSC-Based Organoid and Chimeric Mouse Models of Down Syndrome. Cell Stem Cell. 2019;: pubmed 出版商
Shah S, Takei Y, Zhou W, Lubeck E, Yun J, Eng C, et al. Dynamics and Spatial Genomics of the Nascent Transcriptome by Intron seqFISH. Cell. 2018;174:363-376.e16 pubmed 出版商
Courtheoux T, Diallo A, Damodaran A, Reboutier D, Watrin E, Prigent C. Aurora A kinase activity is required to maintain an active spindle assembly checkpoint during prometaphase. J Cell Sci. 2018;131: pubmed 出版商
Szuperák M, Churgin M, Borja A, Raizen D, Fang Yen C, Kayser M. A sleep state in Drosophila larvae required for neural stem cell proliferation. elife. 2018;7: pubmed 出版商
Welty S, Teng Y, Liang Z, Zhao W, Sanders L, Greenamyre J, et al. RAD52 is required for RNA-templated recombination repair in post-mitotic neurons. J Biol Chem. 2018;293:1353-1362 pubmed 出版商
Sozmen M, Devrim A, Kabak Y, Devrim T, Sudagidan M. The Effects of Periostin in a Rat Model of Isoproterenol: Mediated Cardiotoxicity. Cardiovasc Toxicol. 2018;18:142-160 pubmed 出版商
图像
图像 1 :
赛默飞世尔 PA5-17869 图像 1
Immunofluorescent analysis of Phospho-Histone H3 pSer10 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or a ToxInsight Instrument at 20X magnification.
图像 2 :
赛默飞世尔 PA5-17869 图像 2
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 10 minutes at 95C. Following antigen-retrieval, tissues were incubated in 3% hydrogen peroxide for 10 minutes, washed, and then blocked in 5% normal goat serum in TBST for 1 hour at room temperature. Tissues were stained with a Phospho-Histone H3 polyclonal antibody (Product # PA5-17869) at a dilution of 1:200 overnight at 4C. Tissues were washed extensively with TBS + 0.1% Tween-20 and detection was performed using a HRP-conjugated secondary reagent followed by DAB substrate.
图像 3 :
赛默飞世尔 PA5-17869 图像 3
Flow cytometric analysis of Phospho-Histone H3 pSer10 on Ramos cells left untreated (blue histogram) or treated with serum/calyculin (green histogram). Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Histone H3 pSer10 polyclonal antibody (Product # PA5-17869) at a dilution of 1:50 for 1 hour at room temperature. As a negative control, serum/calyculin-treated cells were stained with an isotype control (red histogram). Following staining with either PA5-17869 or isotype control, cells were stained with a fluorescently-conjugated anti-rabbit IgG secondary antibody and analyzed on a flow cytometer.
产品信息
产品类型 :
抗体
产品名称 :
磷酸化组蛋白H3(Ser10)多克隆抗体
目录# :
PA5-17869
规格 :
100微升
价格 :
美国445.00
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
反应物种 :
Drosophila, Human, Mouse, Non-human primate, Rat, Xenopus laevis
应用 :
Flow Cytometry: 1:50, Immunocytochemistry: 1:800, Immunohistochemistry (Frozen): 1:400, Immunohistochemistry (Paraffin): 1:200, Peptide array: 1:2,000, Western Blot: 1:1,000
物种 :
Drosophila, Human, Mouse, Non-human primate, Rat, Xenopus laevis
抗体亚型 :
IgG
储存 :
-20°C
描述 :
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
免疫原 :
Synthetic phosphopeptide corresponding to residues surrounding pSer10 of human histone H3
格式 :
液体
应用w/稀释 :
Flow Cytometry: 1:50, Immunocytochemistry: 1:800, Immunohistochemistry (Frozen): 1:400, Immunohistochemistry (Paraffin): 1:200, Peptide array: 1:2,000, Western Blot: 1:1,000
别名 :
BUR5; CG31613; CG31613-PA; CG33803; CG33806; CG33809; CG33812; CG33815; CG33818; CG33821; CG33824; CG33827; CG33830; CG33833; CG33836; CG33839; CG33842; CG33845; CG33848; CG33851; CG33854; CG33857; CG33860; CG33863; CG33866; CG5825; CG5825-PA; CG5825-PC; CG5825-PD; CG8989; dH3.3A; Dmel CG31613; Dmel CG5825; Dmel_CG31613; Dmel_CG5825; fb58e10; h3; H3 histone; H3 histone family, member A; H3 histone family, member I; H3 histone family, member K; H3 histone family, member L; H3 histone family, member M; H3 histone, family 2; H3 histone, family 3A; H3 histone, family 3B; H3 histone, family 3B (H3.3B); H3 histone, family 3B.1; H3 histone, family 3C; H3.1-221; H3.1-291; H3.1-I; H3.2; H3.2-221; H3.2-614; H3.2-615; H3.2-616; h3.2a; H3.3; H3.3 histone A; H3.3 histone B; H3.3a; H3.3B; H3.5; H3.5 histone; H3.A; H3.B; H3/A; H3/b; H3/d; H3/f; H3/i; H3/j; H3/k; H3/l; H3/M; H3/n; H3/o; H3-143; H3-291; H3-3A; H3-3B; H3-5; H3-53; H3-614; H3a; H3b; H3-B; H3C1; H3c10; H3c11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3f; H3-F; H3F1K; H3F2; H3F3; h3f3a; H3F3A protein; H3f3b; h3f3b.1; H3F3C; h3f3d; H3FA; H3FB; H3FC HIST1H3C; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; H3FM; H3FN; H3g; H3h; H3i; H3L-like histone; h3r; H3S10ph; H4 clustered histone 16; H4 clustered histone 19; H4C16; H4C19; HHT1; HHT2; His3; His3.3; His3.3A; His-3.3A; His3.3A-PA; His3.3A-PC; His3.3A-PD; His3.3B; His3:CG31613; His3:CG31613-PA; His3:CG33803; His3:CG33806; His3:CG33809; His3:CG33812; His3:CG33815; His3:CG33818; His3:CG33821; His3:CG33824; His3:CG33827; His3:CG33830; His3:CG33833; His3:CG33836; His3:CG33839; His3:CG33842; His3:CG33845; His3:CG33848; His3:CG33851; His3:CG33854; His3:CG33857; His3:CG33860; His3:CG33863; His3:CG33866; Hist1; Hist1h2ai; Hist1h2ail; Hist1h2ail1; HIST1H3A; HIST1H3B; Hist1h3c; HIST1H3D; Hist1h3e; Hist1h3f; Hist1h3g; hist1h3g.L; HIST1H3H; HIST1H3I; HIST1H3J; hist2h3; HIST2H3A; Hist2h3b; HIST2H3C; Hist2h3c1; Hist2h3c2; Hist2h3c2-ps; Hist2h3ca1; Hist2h3ca2; HIST2H3D; histone; histone 1, H2ai; histone 1, H3a; histone 1, H3b; histone 1, H3f; histone 1, H3h; histone 2, H3a; histone 2, H3c; histone 2, H3c2; histone 2, H3ca2; Histone 3; histone cluster 1 H3 family member a; histone cluster 1, H2ai; histone cluster 1, H2ai-like; histone cluster 1, H2ai-like1; histone cluster 1, H3a; histone cluster 1, H3b; histone cluster 1, H3f; histone cluster 1, H3g protein L homeolog; histone cluster 1, H3h; Histone Cluster 2 H3a; histone cluster 2, H3a; histone cluster 2, H3c; histone cluster 2, H3c2; histone cluster 2, H3c2, pseudogene; histone cluster 3, H3; histone gene complex 1; histone H3; Histone H3 containing protein; histone H3.1; Histone H3.2; histone H3.2-like; histone H3.3; Histone H3.3A; Histone H3.3B; Histone H3.3C; histone H3.3C-like; histone H3.3-like protein; Histone H3.5; histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; histone H3/l; Histone H3/m; histone H3/o; histone H3-like; histone H4; histone variant H3.5; hypothetical protein LOC406269; hypothetical protein LOC550262; I79_014844; lamprey; lpy; M32461; N2749; PH3; PP781; similar to H3 histone, family 3A; SIN2; Unknown (protein for MGC:128166); wu:fa25h06; wu:fa96g06; wu:fb07a08; wu:fb36f01; wu:fb58e10; XELAEV_18028537mg; YBR010W; YBR0201; YNL031C; zgc:110292; zgc:174300; zgc:56193; zgc:56418; zgc:64222; zgc:86731
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国