产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
甲平滑肌肌动蛋白多克隆抗体
目录 :
PA5-19465
规格 :
100µg
价格 :
399.00美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠, 斑马鱼, 国内马
应用 :
免疫印迹, 免疫组化, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
赛默飞世尔 产品网页
文章摘录数: 7
出版应用/物种/样本/稀释参考文献
  • 免疫组化-石蜡切片; 小鼠; 1:100; 图 4c
  • 免疫组化-石蜡切片; 人类; 1:100; 图 1a
Biasin V, Wygrecka M, Marsh L, Becker Pauly C, Brcic L, Ghanim B, et al. Meprin β contributes to collagen deposition in lung fibrosis. Sci Rep. 2017;7:39969 pubmed 出版商
Pattanaik S, Arbra C, Bainbridge H, Dennis S, Fann S, Yost M. Vascular Tissue Engineering Using Scaffold-Free Prevascular Endothelial-Fibroblast Constructs. Biores Open Access. 2019;8:1-15 pubmed 出版商
Xie X, Percipalle P. Elevated transforming growth factor ? signaling activation in ?-actin-knockout mouse embryonic fibroblasts enhances myofibroblast features. J Cell Physiol. 2018;: pubmed 出版商
Reebye V, Huang K, Lin V, Jarvis S, Cutilas P, Dorman S, et al. Gene activation of CEBPA using saRNA: preclinical studies of the first in human saRNA drug candidate for liver cancer. Oncogene. 2018;37:3216-3228 pubmed 出版商
Toghill B, Saratzis A, Freeman P, Sylvius N, Bown M. SMYD2 promoter DNA methylation is associated with abdominal aortic aneurysm (AAA) and SMYD2 expression in vascular smooth muscle cells. Clin Epigenetics. 2018;10:29 pubmed 出版商
Ntokou A, Szibor M, Rodríguez Castillo J, Quantius J, Herold S, El Agha E, et al. A novel mouse Cre-driver line targeting Perilipin 2-expressing cells in the neonatal lung. Genesis. 2017;55: pubmed 出版商
Higuchi A, Kao S, Ling Q, Chen Y, Li H, Alarfaj A, et al. Long-term xeno-free culture of human pluripotent stem cells on hydrogels with optimal elasticity. Sci Rep. 2015;5:18136 pubmed 出版商
图像
图像 1 :
赛默飞世尔 PA5-19465 图像 1
Western blot analysis of MEF1 Whole Cell Lysate using PA5-19465, alpha smooth muscle Actin primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19465 staining of Rat Brain Tissue Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
图像 2 :
赛默飞世尔 PA5-19465 图像 2
Immunofluorescent staining of HeLa cells using PA5-19465, anti-Alpha smooth muscle Actin antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 20 minutes and exposed to the primary antibody at a concentration of 10 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
产品信息
产品类型 :
抗体
产品名称 :
甲平滑肌肌动蛋白多克隆抗体
目录# :
PA5-19465
规格 :
100µg
价格(美元) :
399.00美元
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
兔
反应物种 :
Equine, Human, Mouse, Rat, Zebrafish
应用 :
Immunohistochemistry (Frozen): 1:1000, Immunohistochemistry (Paraffin): Assay-Dependent, Immunohistochemistry (PFA fixed): 1:200, Western Blot: 1 µg/mL
物种 :
Equine, Human, Mouse, Rat, Zebrafish
抗体亚型 :
IgG
储存 :
-20°C or -80°C if preferred
描述 :
Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actin being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown that smooth muscle actin appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases. Multiple alternatively spliced variants of smooth muscle actin have been identified.
免疫原 :
Synthetic peptide derived from within residues 1 - 100 of Human alpha smooth muscle Actin.
格式 :
液体
应用w/稀释 :
Immunohistochemistry (Frozen): 1:1000, Immunohistochemistry (Paraffin): Assay-Dependent, Immunohistochemistry (PFA fixed): 1:200, Western Blot: 1 µg/mL
别名 :
0610041G09Rik; aat6; ACT-4; Acta2; acta2.L; acta2.S; actin; actin alpha 2, smooth muscle; actin, alpha 2, smooth muscle, aorta; actin, alpha 2, smooth muscle, aorta L homeolog; actin, alpha 2, smooth muscle, aorta S homeolog; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; ACTSA; Actvs; alpha actin 2; alpha smooth muscle actin; alpha-actin; Alpha-actin-2; alpha-cardiac actin; alphaSMA; alpha-SM-actin; alpha-smooth muscle actin; ASMA; a-sma; Cell growth-inhibiting gene 46 protein; GIG46; hypothetical protein LOC515610; LOW QUALITY PROTEIN: actin, aortic smooth muscle; mymy5; SM actin; sma; SMactin; sm-actin; SMalphaA; smooth muscle; smooth muscle alpha-actin; vascular smooth muscle alpha-actin; wu:fb63d03; XELAEV_18034370mg
更多信息或购买 :
赛默飞世尔 产品网页
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国