产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
CDK9多克隆抗体
目录 :
PA5-19674
规格 :
200微升
价格 :
美国446.00
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Western blot analysis of A431 Whole Cell Lysate using PA5-19674, Cdk9 primary antibody at a dilution of 1:250 (lane 1). PA5-19674 staining of HEK293 Whole Cell Lysate at a dilution of 1:250 (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
图像 2 :
Immunofluorescent staining of HeLa cells using PA5-19674, anti-Cdk9 antibody. The cells were fixed with PFA (4%) for 10 minutes, permabilised with BSA(1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 20 minutes and exposed to the primary antibody at a concentration of 1 ug/ml for 1 hour at room temp. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
产品信息
产品类型 :
抗体
产品名称 :
CDK9多克隆抗体
目录# :
PA5-19674
规格 :
200微升
价格 :
美国446.00
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
兔
反应物种 :
人类, 小鼠, 非洲爪蛙
应用 :
Immunocytochemistry: 1 µg/mL, Western Blot: 1:250-1:500
物种 :
人类, 小鼠, 非洲爪蛙
抗体亚型 :
IgG
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
CDK9 is a serine/threonine kinase which when complexed with CyclinT1 forms an active holoenzyme complex. This complex plays a key role in regulating the eukaryotic cell cycle. CDK9 is coexpressed and copurified with CyclinT1. CDK9 regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and confers a predisposition to heart failure.
免疫原 :
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Cdk9.
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1 µg/mL, Western Blot: 1:250-1:500
别名 :
C-2k; CDC2L4; CDC2-related kinase; Cdk9; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; CTK1; cyclin dependent kinase 9; cyclin-dependent kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); PITALRE; RP11-228B15.5; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; TAK; Tat-associated kinase complex catalytic subunit
更多信息或购买 :
公司信息
赛默飞世尔