NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

抗体

LC3A/LC3B Polyclonal Antibody, DyLight™ 550

PA5-22733

100微升

美国455.00

多克隆

domestic rabbit

DyLight系列染料550

人类, 小鼠, 大鼠, 犬, 斑马鱼

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片

LC3A/LC3B Polyclonal Antibody, DyLight™ 550

PA5-22733

100微升

美国455.00

多克隆

抗原亲和色谱

兔

两栖动物, 狗, 鱼类, 人类, 小鼠, 植物, 大鼠, 斑马鱼

ELISA: Assay-Dependent, Flow Cytometry: Assay-Dependent, Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Frozen): Assay-Dependent, Immunohistochemistry (Paraffin): 1:200-1:400, Western Blot: 2 µg/mL

两栖动物, 狗, 鱼类, 人类, 小鼠, 植物, 大鼠, 斑马鱼

IgG

4° C, store in dark

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121).

液体

ELISA: Assay-Dependent, Flow Cytometry: Assay-Dependent, Immunocytochemistry: Assay-Dependent, Immunohistochemistry (Frozen): Assay-Dependent, Immunohistochemistry (Paraffin): 1:200-1:400, Western Blot: 2 µg/mL

1010001C15Rik; 1010001H21Rik; 4922501H04Rik; Atg8; ATG8E; ATG8F; Autophagy-related protein LC3 A; autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 A; autophagy-related ubiquitin-like modifier LC3 B; LC3; LC3a; LC3b; MAP1 light chain 3-like protein 1; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B light chain 3 B; Map1alc3; MAP1BLC3; Map1lc3; Map1lc3a; MAP1LC3B; MAP1LC3B-a; microtubule associated protein 1 light chain 3 alpha; microtubule associated protein 1 light chain 3 beta; microtubule-associated protein 1 light chain 3; microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1-light chain 3A; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3A; Microtubule-associated proteins 1A/1B light chain 3B; Mpl3; wu:fb60g11; zbs559; zgc:56434; zgc:77094