产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
增殖细胞核抗原多克隆抗体
目录 :
PA5-27214
规格 :
100微升
价格 :
美国496.00
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
中国人仓鼠, 人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 16
出版应用/物种/样本/稀释 | 参考文献 |
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Bersani Amado L, da Rocha B, Schneider L, Ames F, Breithaupt Faloppa A, Araújo G, et al. Nephropathy induced by renal microembolism: a characterization of biochemical and histopathological changes in rats. Int J Clin Exp Pathol. 2019;12:2311-2323 pubmed
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图像
图像 1 :
Western Blot analysis using anti-PCNA Polyclonal Antibody on the following cells: (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG. Primary antibody (PA5-27214) diluted at 1:3000
图像 2 :
Confocal immunofluorescence analysis of methanol-fixed HeLa using PCNA (PA5-27214) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with MA1-25054 (Red) at 1:500.
图像 3 :
Immunohistochemical analysis of paraffin-embedded KYSE170 xenograft using PCNA (PA5-27214) antibody at 1:500 dilution.
产品信息
产品类型 :
抗体
产品名称 :
增殖细胞核抗原多克隆抗体
目录# :
PA5-27214
规格 :
100微升
价格 :
美国496.00
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
兔
反应物种 :
仓鼠, 人类, 小鼠, 大鼠
应用 :
Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, In Situ Hybridization (ISH): Assay-dependent, Western Blot: 1:500-1:3,000
物种 :
仓鼠, 人类, 小鼠, 大鼠
抗体亚型 :
IgG
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
免疫原 :
Recombinant fragment corresponding to a region within amino acids 1 and 253 of Human PCNA
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, In Situ Hybridization (ISH): Assay-dependent, Western Blot: 1:500-1:3,000
别名 :
ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; hypothetical protein LOC515499; MGC8367; Pcna; pcna protein; Pcna/cyclin; PCNAR; POL30; Proliferating cell nuclear antigen; wu:fa28e03; wu:fb36g03; YBR0811; YBR088C
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公司信息
赛默飞世尔