产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
增殖细胞核抗原多克隆抗体
目录 :
PA5-27214
规格 :
100微升
价格 :
美国496.00
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
中国人仓鼠, 人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 16
出版应用/物种/样本/稀释参考文献
  • 免疫组化-石蜡切片; 大鼠; 1:200; 图 1d
Helle J, Keiler A, Zierau O, Dörfelt P, Vollmer G, Lehmann L, et al. Effects of the aryl hydrocarbon receptor agonist 3-methylcholanthrene on the 17?-estradiol regulated mRNA transcriptome of the rat uterus. J Steroid Biochem Mol Biol. 2017;171:133-143 pubmed 出版商
  • 免疫细胞化学; 小鼠; 1:100; 图 2b
Bartova E, Suchankova J, Legartova S, Malyšková B, Hornáček M, Skalníková M, et al. PCNA is recruited to irradiated chromatin in late S-phase and is most pronounced in G2 phase of the cell cycle. Protoplasma. 2017;254:2035-2043 pubmed 出版商
Wasbotten R, Dahler A, Mackel J, Morffy Smith C, Rosen D. Murine Respiratory Tract Infection with Classical Klebsiella pneumoniae Induces Bronchus-Associated Lymphoid Tissue. Infect Immun. 2022;90:e0059621 pubmed 出版商
Isaeva E, Beketov E, Demyashkin G, Yakovleva N, Arguchinskaya N, Kisel A, et al. Cartilage Formation In Vivo Using High Concentration Collagen-Based Bioink with MSC and Decellularized ECM Granules. Int J Mol Sci. 2022;23: pubmed 出版商
Feng C, Wang Q, Deng L, Peng N, Yang M, Wang X. Hsa_circ_0074032 promotes prostate cancer progression through elevating homeobox A1 expression by serving as a microRNA-198 decoy. Andrologia. 2022;54:e14312 pubmed 出版商
He Z, Lei S, Liang F, Tan L, Zhang W, Xie L, et al. Low-Dose Albendazole Inhibits Epithelial-Mesenchymal Transition of Melanoma Cells by Enhancing Phosphorylated GSK-3β/Tyr216 Accumulation. J Oncol. 2021;2021:4475192 pubmed 出版商
Kassa B, Kumar R, Mickael C, Sanders L, Vohwinkel C, Lee M, et al. Endothelial cell PHD2-HIF1α-PFKFB3 contributes to right ventricle vascular adaptation in pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol. 2021;321:L675-L685 pubmed 出版商
Li S, Jiang T, Cao Q, Liu S. Transmembrane protein ADAM29 facilitates cell proliferation, invasion and migration in clear cell renal cell carcinoma. J Chemother. 2021;33:40-50 pubmed 出版商
An Y, Liu H, Zhou Z, Wang J, Jiang G, Li Z, et al. Sinoporphyrin sodium is a promising sensitizer for photodynamic and sonodynamic therapy in glioma. Oncol Rep. 2020;44:1596-1604 pubmed 出版商
Mintie C, Musarra A, Singh C, Ndiaye M, Sullivan R, Eickhoff J, et al. Protective Effects of Dietary Grape on UVB-Mediated Cutaneous Damages and Skin Tumorigenesis in SKH-1 Mice. Cancers (Basel). 2020;12: pubmed 出版商
Bersani Amado L, da Rocha B, Schneider L, Ames F, Breithaupt Faloppa A, Araújo G, et al. Nephropathy induced by renal microembolism: a characterization of biochemical and histopathological changes in rats. Int J Clin Exp Pathol. 2019;12:2311-2323 pubmed
Ragy M, Ahmed S. Protective effects of either C-peptide or l-arginine on pancreatic β-cell function, proliferation, and oxidative stress in streptozotocin-induced diabetic rats. J Cell Physiol. 2019;234:11500-11510 pubmed 出版商
Abe M, Yoshida T, Akiba J, Ikezono Y, Wada F, Masuda A, et al. STAT3 deficiency prevents hepatocarcinogenesis and promotes biliary proliferation in thioacetamide-induced liver injury. World J Gastroenterol. 2017;23:6833-6844 pubmed 出版商
Sozmen M, Devrim A, Kabak Y, Devrim T, Sudagidan M. The Effects of Periostin in a Rat Model of Isoproterenol: Mediated Cardiotoxicity. Cardiovasc Toxicol. 2018;18:142-160 pubmed 出版商
Hasan S, Sultana S. Geraniol attenuates 2-acetylaminofluorene induced oxidative stress, inflammation and apoptosis in the liver of wistar rats. Toxicol Mech Methods. 2015;25:559-73 pubmed 出版商
Gronowicz G, Secor E, Flynn J, Jellison E, Kuhn L. Therapeutic Touch Has Significant Effects on Mouse Breast Cancer Metastasis and Immune Responses but Not Primary Tumor Size. Evid Based Complement Alternat Med. 2015;2015:926565 pubmed 出版商
图像
图像 1 :
赛默飞世尔 PA5-27214 图像 1
Western Blot analysis using anti-PCNA Polyclonal Antibody on the following cells: (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG. Primary antibody (PA5-27214) diluted at 1:3000
图像 2 :
赛默飞世尔 PA5-27214 图像 2
Confocal immunofluorescence analysis of methanol-fixed HeLa using PCNA (PA5-27214) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with MA1-25054 (Red) at 1:500.
图像 3 :
赛默飞世尔 PA5-27214 图像 3
Immunohistochemical analysis of paraffin-embedded KYSE170 xenograft using PCNA (PA5-27214) antibody at 1:500 dilution.
产品信息
产品类型 :
抗体
产品名称 :
增殖细胞核抗原多克隆抗体
目录# :
PA5-27214
规格 :
100微升
价格 :
美国496.00
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
反应物种 :
仓鼠, 人类, 小鼠, 大鼠
应用 :
Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, In Situ Hybridization (ISH): Assay-dependent, Western Blot: 1:500-1:3,000
物种 :
仓鼠, 人类, 小鼠, 大鼠
抗体亚型 :
IgG
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.
免疫原 :
Recombinant fragment corresponding to a region within amino acids 1 and 253 of Human PCNA
格式 :
液体
应用w/稀释 :
Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Frozen): Assay-dependent, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, In Situ Hybridization (ISH): Assay-dependent, Western Blot: 1:500-1:3,000
别名 :
ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690.10; fa28e03; fb36g03; HGCN8729; hypothetical protein LOC515499; MGC8367; Pcna; pcna protein; Pcna/cyclin; PCNAR; POL30; Proliferating cell nuclear antigen; wu:fa28e03; wu:fb36g03; YBR0811; YBR088C
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国