产品简要
公司名称 :
赛默飞世尔
其他品牌 :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
产品类型 :
抗体
产品名称 :
p53多克隆抗体
目录 :
PA5-27822
规格 :
100微升
价格 :
美国399.00
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片
更多信息或购买 :
文章摘录数: 2
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 人类; 1:50; 图 5a
Yu G, Dou Z, Jia Z. 5?bromo?3?(3?hydroxyprop?1?ynyl)?2H?pyran?2?one induces apoptosis in T24 human bladder cancer cells through mitochondria-dependent signaling pathways. Mol Med Rep. 2017;15:153-159 pubmed 出版商
Juillard F, de Miranda M, Li S, Franco A, Seixas A, Liu B, et al. KSHV LANA acetylation-selective acidic domain reader sequence mediates virus persistence. Proc Natl Acad Sci U S A. 2020;117:22443-22451 pubmed 出版商
图像
图像 1 :
赛默飞世尔 PA5-27822 图像 1
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft using p53 (PA5-27822) antibody at 1:500 dilution.
图像 2 :
赛默飞世尔 PA5-27822 图像 2
Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS using p53 (PA5-27822) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with MA1-25054 (Red) at 1:2000.
图像 3 :
赛默飞世尔 PA5-27822 图像 3
Western Blot analysis using anti-p53 Polyclonal Antibody on the following cells: (30 ug of whole cell lysate) A: HCT116 cells with mock treatment for 24hr B: HCT116 cells with 30uM cisplatin treatment for 24hr. Primary antibody (PA5-27822) diluted at 1:1000
产品信息
产品类型 :
抗体
产品名称 :
p53多克隆抗体
目录# :
PA5-27822
规格 :
100微升
价格 :
美国399.00
克隆性 :
多克隆
纯度 :
抗原亲和色谱
宿主 :
反应物种 :
人类, 小鼠
应用 :
ChIP assay: Assay-dependent, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, Western Blot: 1:500-1:3,000
物种 :
人类, 小鼠
抗体亚型 :
IgG
储存 :
短期存放在4°C。 为长期储存, 存放在-20°C, 避免反复冷冻/解冻
描述 :
The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.
免疫原 :
Recombinant fragment corresponding to a region within amino acids 142 and 393 of Human p53
格式 :
液体
应用w/稀释 :
ChIP assay: Assay-dependent, Immunocytochemistry: 1:100-1:1,000, Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: 1:100-1:500, Western Blot: 1:500-1:3,000
别名 :
Antigen NY-CO-13; bbl; BCC7; bfy; bhy; cellular tumor antigen p53; Cys 51 Stop; EGK_08142; FLJ92943; HGNC11998; I79_002739; LFS1; Li-Fraumeni syndrome; mutant p53; mutant tumor protein 53; OTTMUSP00000006194; p44; p53; p53 cellular tumor antigen; p53 protein; p53 tumor suppressor; p53 tumor suppressor phosphoprotein; phosphoprotein p53; Tp53; tp53.L; transformation related protein 53; transformation-related protein 53; Trp248; Trp53; tumor protein 53; tumor protein p53; tumor protein p53 (Li-Fraumeni syndrome); tumor protein p53 L homeolog; tumor suppressor p53; tumor suppressor p53 phosphoprotein; tumor suppressor protein p53; tumor supressor p53; Tumour Protein p53; XELAEV_180196761mg; Xp53; Xrel3
更多信息或购买 :
公司信息
赛默飞世尔
上海浦东新金桥路27号7号楼
analyze.cn@thermofisher.com
http://www.thermo.com.cn
1084193588
公司总部: 美国