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抗体

磷酸化胰岛素受体底物1(Ser323)多克隆抗体

PA5-40280

100微升

美国446.00

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学

磷酸化胰岛素受体底物1(Ser323)多克隆抗体

PA5-40280

100微升

美国446.00

多克隆

抗原亲和色谱

兔

Human, Mouse, Non-human primate, Rat

ELISA: 1:10,000, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry: 1:100-1:300, Western Blot: 1:500-1:2,000

Human, Mouse, Non-human primate, Rat

IgG

-20° C, 避免反复冷冻/解冻

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

Synthesized peptide derived from human IRS-1 around the phosphorylation site of S323.

液体

ELISA: 1:10,000, Immunocytochemistry: 1:200-1:1,000, Immunohistochemistry: 1:100-1:300, Western Blot: 1:500-1:2,000

G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185