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IHC-plus™ PLK1 / PLK-1 Antibody (phospho-Thr210) LS-B50
LifeSpan Biosciences
目录: LS-B50
domestic rabbit 多克隆
反应物种:
人类
修饰: 磷酸化
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
免疫组化-石蜡切片
Anti-PLK1 antibody IHC of human lung carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
规格:
价格:
至产商
IHC-plus™ PLK1 / PLK-1 Antibody (phospho-Thr210, clone 2A3) LS-B1675
LifeSpan Biosciences
目录: LS-B1675
小鼠 单克隆 (2A3)
反应物种:
人类
修饰: 磷酸化
应用:
免疫印迹
,
免疫组化
,
免疫组化-石蜡切片
文章摘录数: 1
Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.
规格:
价格:
至产商
IHC-plus™ PLK1 / PLK-1 Antibody LS-B4225
LifeSpan Biosciences
目录: LS-B4225
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫组化-石蜡切片
Sample(30 ug whole cell lysate). A: Raji. 7.5% SDS PAGE. PLK1 antibody diluted at 1:1000.
Anti-PLK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
规格:
价格:
至产商
PLK1/PLK-1抗体(克隆OTI3F12)LS-C114491
LifeSpan Biosciences
目录: LS-C114491
小鼠 单克隆 (OTI3F12)
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫沉淀
,
流式细胞仪
,
免疫组化-石蜡切片
文章摘录数: 1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.
IHC of paraffin-embedded liver tissue using anti-PLK1 mouse monoclonal antibody. (Dilution 1:50).
Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
规格:
价格:
至产商
PLK1/PLK-1抗体LS-C9648
LifeSpan Biosciences
目录: LS-C9648
小鼠 单克隆
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫细胞化学
,
免疫沉淀
规格:
价格:
至产商
PLK1/PLK-1抗体LS-C9649
LifeSpan Biosciences
目录: LS-C9649
小鼠 单克隆
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫细胞化学
,
免疫沉淀
规格:
价格:
至产商
PLK1/PLK-1抗体(aa402-603)LS-C17320
LifeSpan Biosciences
目录: LS-C17320
小鼠 单克隆
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫细胞化学
,
免疫沉淀
规格:
价格:
至产商
PLK1/PLK-1抗体(克隆3F8)LS-C40938
LifeSpan Biosciences
目录: LS-C40938
小鼠 单克隆 (3F8)
反应物种:
人类
应用:
免疫印迹
,
免疫沉淀
Hela cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Plk-1 (Clone 3F8) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to.
规格:
价格:
至产商
PLK1/PLK-1抗体(内部, 克隆3F8)LS-C63154
LifeSpan Biosciences
目录: LS-C63154
小鼠 单克隆 (3F8)
反应物种:
人类
应用:
免疫印迹
规格:
价格:
至产商
PLK1/PLK-1抗体(aa573-603)LS-C99728
LifeSpan Biosciences
目录: LS-C99728
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫组化-石蜡切片
Western blot of anti-PLK antibody in A375 cell lysate. PLK (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
规格:
价格:
至产商
PLK1/PLK-1抗体(aa176-225)LS-C117697
LifeSpan Biosciences
目录: LS-C117697
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
Immunofluorescence analysis of A549 cells, using PLK1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from LOVO cells, treated with PMA 125ng/ml 30', using PLK1 Antibody. The lane on the right is blocked with the synthesized peptide.
规格:
价格:
至产商
IHC-plus™ PLK1 / PLK-1 Antibody (C-Terminus) LS-B5792
LifeSpan Biosciences
目录: LS-B5792
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫组化-石蜡切片
Anti-PLK1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
规格:
价格:
至产商
PLK1/PLK-1抗体(N末端)LS-C146512
LifeSpan Biosciences
目录: LS-C146512
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
规格:
价格:
至产商
IHC-plus™ PLK1 / PLK-1 Antibody (aa100-200) LS-B6508
LifeSpan Biosciences
目录: LS-B6508
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫组化-石蜡切片
Anti-PLK1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
规格:
价格:
至产商
IHC-plus™ PLK1 / PLK-1 Antibody LS-B6646
LifeSpan Biosciences
目录: LS-B6646
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫组化-石蜡切片
Anti-PLK1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
规格:
价格:
至产商
PLK1/PLK-1抗体(克隆1D1)LS-C169107
LifeSpan Biosciences
目录: LS-C169107
小鼠 单克隆 (1D1)
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫细胞化学
规格:
价格:
至产商
PLK1/PLK-1抗体(克隆3C11)LS-C169110
LifeSpan Biosciences
目录: LS-C169110
小鼠 单克隆 (3C11)
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
流式细胞仪
规格:
价格:
至产商
PLK1/PLK-1抗体(克隆OTI1D4)LS-C114495
LifeSpan Biosciences
目录: LS-C114495
小鼠 单克隆 (OTI1D4)
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫沉淀
,
免疫组化-石蜡切片
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-PLK1 mouse monoclonal antibody. (Dilution 1:50).
Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
规格:
价格:
至产商
PLK1/PLK-1抗体(克隆OTI8C12)LS-C114499
LifeSpan Biosciences
目录: LS-C114499
小鼠 单克隆 (OTI8C12)
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
免疫沉淀
,
流式细胞仪
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PLK1 monoclonal antibody.
Anti-PLK1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PLK1.
Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
规格:
价格:
至产商
PLK1/PLK-1抗体(克隆OTI5C1)LS-C114502
LifeSpan Biosciences
目录: LS-C114502
小鼠 单克隆 (OTI5C1)
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫沉淀
,
流式细胞仪
,
免疫组化-石蜡切片
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.
IHC of paraffin-embedded colon tissue using anti-PLK1 mouse monoclonal antibody. (Dilution 1:50).
Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
规格:
价格:
至产商
PLK1/PLK-1抗体(克隆OTI6C6)LS-C114507
LifeSpan Biosciences
目录: LS-C114507
小鼠 单克隆 (OTI6C6)
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫沉淀
,
免疫组化-石蜡切片
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PLK1 monoclonal antibody.
IHC of paraffin-embedded colon tissue using anti-PLK1 mouse monoclonal antibody. (Dilution 1:50).
Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
规格:
价格:
至产商
PLK1/PLK-1抗体(克隆OTI9E9)LS-C172325
LifeSpan Biosciences
目录: LS-C172325
小鼠 单克隆 (OTI9E9)
反应物种:
人类
应用:
免疫印迹
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.
规格:
价格:
至产商
PLK1/PLK-1抗体(aa287-298)LS-C186577
LifeSpan Biosciences
目录: LS-C186577
domestic goat 多克隆
反应物种:
人类
应用:
免疫印迹
PLK1 antibody (0.3 ug/ml) staining of HeLa nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
规格:
价格:
至产商
PLK1/PLK-1抗体(aa300-603, 克隆1D4B)LS-C189750
LifeSpan Biosciences
目录: LS-C189750
小鼠 单克隆 (1D4B)
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
免疫沉淀
规格:
价格:
至产商
PLK1/PLK-1抗体(克隆2G12)LS-C197924
LifeSpan Biosciences
目录: LS-C197924
小鼠 单克隆 (2G12)
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫细胞化学
,
免疫沉淀
Western blot analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.
Immunofluorescence of monoclonal antibody to PLK1 on HeLa cell. [antibody concentration 10 ug/ml]
Immunoprecipitation of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 rabbit polyclonal antibody.
规格:
价格:
至产商
PLK1/PLK-1抗体(磷酸化Thr210)LS-C199481
LifeSpan Biosciences
目录: LS-C199481
domestic rabbit 多克隆
反应物种:
人类
修饰: 磷酸化
应用:
免疫组化
,
免疫组化-石蜡切片
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PLK1 (Phospho-Thr210) Antibody. The picture on the right is blocked with the phospho peptide.
规格:
价格:
至产商
PLK1/PLK-1抗体(aa573-603, AP)LS-C266556
LifeSpan Biosciences
目录: LS-C266556
domestic rabbit 多克隆
反应物种:
人类
共轭标签: AP
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
规格:
价格:
至产商
PLK1/PLK-1抗体(aa573-603, APC)LS-C266557
LifeSpan Biosciences
目录: LS-C266557
domestic rabbit 多克隆
反应物种:
人类
共轭标签: APC
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
规格:
价格:
至产商
PLK1/PLK-1抗体(aa573-603, 生物素)LS-C266558
LifeSpan Biosciences
目录: LS-C266558
domestic rabbit 多克隆
反应物种:
人类
共轭标签: 生物素
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
规格:
价格:
至产商
PLK1/PLK-1抗体(aa573-603, 异硫氰酸荧光素)LS-C266559
LifeSpan Biosciences
目录: LS-C266559
domestic rabbit 多克隆
反应物种:
人类
共轭标签: 异硫氰酸荧光素
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
规格:
价格:
至产商
PLK1/PLK-1抗体(aa573-603, 无叠氮物, HRP)LS-C266560
LifeSpan Biosciences
目录: LS-C266560
domestic rabbit 多克隆
反应物种:
人类
共轭标签: HRP
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
规格:
价格:
至产商
PLK1/PLK-1抗体(aa573-603, PE)LS-C266566
LifeSpan Biosciences
目录: LS-C266566
domestic rabbit 多克隆
反应物种:
人类
共轭标签: PE
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
规格:
价格:
至产商
PLK1/PLK-1抗体(aa325-375)LS-C286344
LifeSpan Biosciences
目录: LS-C286344
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫细胞化学
规格:
价格:
至产商
PLK1/PLK-1抗体(aa1-50)LS-C287162
LifeSpan Biosciences
目录: LS-C287162
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫沉淀
规格:
价格:
至产商
PLK1/PLK-1抗体(aa325-375)LS-C287163
LifeSpan Biosciences
目录: LS-C287163
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
,
免疫沉淀
规格:
价格:
至产商
PLK1/PLK-1抗体(磷酸化Ser137)LS-C291191
LifeSpan Biosciences
目录: LS-C291191
domestic rabbit 多克隆
反应物种:
人类
修饰: 磷酸化
应用:
免疫印迹
规格:
价格:
至产商
PLK1/PLK-1抗体(aa573-603)LS-C324113
LifeSpan Biosciences
目录: LS-C324113
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
规格:
价格:
至产商
PLK1/PLK-1抗体LS-C332156
LifeSpan Biosciences
目录: LS-C332156
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
Western blot analysis of extracts of various cell lines.
Immunofluorescence analysis of U2OS cells.
规格:
价格:
至产商
PLK1/PLK-1抗体LS-C341284
LifeSpan Biosciences
目录: LS-C341284
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫细胞化学
规格:
价格:
至产商
PLK1/PLK-1抗体(磷酸化Thr210)LS-C354059
LifeSpan Biosciences
目录: LS-C354059
domestic rabbit 多克隆
反应物种:
人类
修饰: 磷酸化
应用:
免疫印迹
,
免疫组化
,
免疫组化-石蜡切片
Western blot analysis of PLK1 (pT210) expression in HeLa (A); A549 (B); K562 (C); COLO320 (D) whole cell lysates.
Immunohistochemical analysis of PLK1 (pT210) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
规格:
价格:
至产商
PLK1/PLK-1抗体(内部)LS-C368472
LifeSpan Biosciences
目录: LS-C368472
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
免疫组化
,
免疫细胞化学
,
免疫组化-石蜡切片
Western blot analysis of PLK1 expression in HeLa (A); mouse hippocampal homogenate (B) whole cell lysates.
Immunohistochemical analysis of PLK1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of PLK1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
规格:
价格:
至产商
PLK1/PLK-1抗体(80-160氨基酸)LS-C381030
LifeSpan Biosciences
目录: LS-C381030
domestic rabbit 多克隆
反应物种:
人类
修饰: 磷酸化
应用:
免疫印迹
,
酶联免疫吸附测定
规格:
价格:
至产商
PLK1/PLK-1抗体(150-230氨基酸)LS-C381555
LifeSpan Biosciences
目录: LS-C381555
domestic rabbit 多克隆
反应物种:
人类
修饰: 磷酸化
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
Western blot of Phospho-Plk (T210) antibody
规格:
价格:
至产商
PLK1/PLK-1抗体(150-230氨基酸)LS-C385588
LifeSpan Biosciences
目录: LS-C385588
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
,
免疫组化
,
免疫细胞化学
规格:
价格:
至产商
PLK1/PLK-1抗体(80-160氨基酸)LS-C386836
LifeSpan Biosciences
目录: LS-C386836
domestic rabbit 多克隆
反应物种:
人类
应用:
免疫印迹
,
酶联免疫吸附测定
Western blot of Plk1 antibody
规格:
价格:
至产商
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