PathPlus™ NANOG Antibody (aa94-123) LS-B3425
LifeSpan Biosciences
目录: LS-B3425
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Anti-NANOG antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

NANOG Antibody western blot of MCF-7 cell line lysates (35 ug/lane). The NANOG antibody detected the NANOG protein (arrow).

Immunofluorescence of NANOG Antibody with HeLa cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
规格:
价格:
至产商
NANOG抗体(生物素)LS-C37434
LifeSpan Biosciences
目录: LS-C37434
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(aa144-157)LS-C55142
LifeSpan Biosciences
目录: LS-C55142
domestic goat 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学

NANOG antibody (0.03µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
规格:
价格:
至产商
NANOG抗体(aa21-70)LS-C81137
LifeSpan Biosciences
目录: LS-C81137
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
规格:
价格:
至产商
NANOG抗体(aa1-154, 克隆5A10)LS-C93768
LifeSpan Biosciences
目录: LS-C93768
小鼠 单克隆 (5A10)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定

The cell lysates of NIH3T3 (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
规格:
价格:
至产商
NANOG抗体(aa1-50)LS-C19389
LifeSpan Biosciences
目录: LS-C19389
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
规格:
价格:
至产商
NANOG抗体(aa24-73)LS-C30739
LifeSpan Biosciences
目录: LS-C30739
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
规格:
价格:
至产商
IHC-plus™ NANOG Antibody (aa291-304) LS-B3017
LifeSpan Biosciences
目录: LS-B3017
domestic goat 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片

Antibody (0.05 ug/ml) staining of HeLa (nuclear) cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-NANOG antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.
规格:
价格:
至产商
NANOG抗体(aa15-49)LS-C98243
LifeSpan Biosciences
目录: LS-C98243
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片

Western blot of anti-NANOG Antibody in K562 cell line lysates (35 ug/lane). NANOG (arrow) was detected using the purified antibody.Western blot of NANOG (arrow) using rabbit polyclonal NANOG Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).

Fluorescent confocal image of SY5Y cells stained NANOG antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated NANOG primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.

Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
规格:
价格:
至产商
NANOG抗体(Ser285)LS-C98246
LifeSpan Biosciences
目录: LS-C98246
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学

Western blot of NANOG (arrow) using rabbit NANOG Antibody (S285). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).

Fluorescent confocal image of SY5Y cells stained NANOG (S285) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated NANOG (S285) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.
规格:
价格:
至产商
NANOG抗体(生物素)LS-C104594
LifeSpan Biosciences
目录: LS-C104594
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体LS-C108536
LifeSpan Biosciences
目录: LS-C108536
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 免疫组化-石蜡切片

Western blot of NANOG in human spleen tissue lysate with NANOG antibody at (A) 1 and (B) 2 ug/ml.

Immunofluorescence of NANOG in Human Spleen cells with NANOG antibody at 20 ug/ml.

Immunohistochemistry of NANOG in human spleen tissue with NANOG antibody at 5 ug/ml.
规格:
价格:
至产商
NANOG抗体(克隆1E6C4)LS-C108845
LifeSpan Biosciences
目录: LS-C108845
小鼠 单克隆 (1E6C4)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
NANOG抗体LS-C109328
LifeSpan Biosciences
目录: LS-C109328
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学

Sample (20 ug ). A: HeLa nucleus. 12% SDS PAGE. NANOG antibody diluted at 1:3000.
规格:
价格:
至产商
NANOG抗体(aa35-84)LS-C112347
LifeSpan Biosciences
目录: LS-C112347
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
规格:
价格:
至产商
NANOG抗体(aa51-100)LS-C120045
LifeSpan Biosciences
目录: LS-C120045
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

Western blot analysis of lysates from HUVEC, HT-29, HepG2, and Jurkat cells, using NANOGP8 Antibody. The lane on the right is blocked with the synthesized peptide.
规格:
价格:
至产商
NANOG抗体(aa1-154)LS-C124221
LifeSpan Biosciences
目录: LS-C124221
小鼠 单克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
规格:
价格:
至产商
NANOG抗体LS-C130968
LifeSpan Biosciences
目录: LS-C130968
小鼠 单克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学
规格:
价格:
至产商
NANOG抗体(aa20-166)LS-C130969
LifeSpan Biosciences
目录: LS-C130969
小鼠 单克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体LS-C130970
LifeSpan Biosciences
目录: LS-C130970
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(aa135-184)LS-C134918
LifeSpan Biosciences
目录: LS-C134918
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
规格:
价格:
至产商
NANOG抗体LS-C146885
LifeSpan Biosciences
目录: LS-C146885
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(C末端)LS-C153465
LifeSpan Biosciences
目录: LS-C153465
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
规格:
价格:
至产商
IHC-plus™ NANOG Antibody LS-B8225
LifeSpan Biosciences
目录: LS-B8225
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片

Anti-NANOG antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
规格:
价格:
至产商
NANOG抗体(磷酸化Ser71)LS-C163646
LifeSpan Biosciences
目录: LS-C163646
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫细胞化学

Fluorescent confocal image of SY5Y cells stained Phospho-Nanog-S71 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated Phospho-Nanog-S71 primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Phospho-Nanog immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.

Dot blot of anti-Phospho-Nanog-S71 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
规格:
价格:
至产商
NANOG抗体(磷酸化Ser285)LS-C203687
LifeSpan Biosciences
目录: LS-C203687
domestic rabbit 多克隆
反应物种: 人类
修饰: 磷酸化

Dot blot of anti-Phospho-Nanog-S285 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
规格:
价格:
至产商
NANOG抗体(Ser285, AP)LS-C263946
LifeSpan Biosciences
目录: LS-C263946
domestic rabbit 多克隆
反应物种: 人类
共轭标签: AP
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
NANOG抗体(Ser285, APC)LS-C263947
LifeSpan Biosciences
目录: LS-C263947
domestic rabbit 多克隆
反应物种: 人类
共轭标签: APC
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
NANOG抗体(Ser285, 异硫氰酸荧光素)LS-C263948
LifeSpan Biosciences
目录: LS-C263948
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
NANOG抗体(Ser285, 无叠氮物, HRP)LS-C263949
LifeSpan Biosciences
目录: LS-C263949
domestic rabbit 多克隆
反应物种: 人类
共轭标签: HRP
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
NANOG抗体(Ser285, PE)LS-C263950
LifeSpan Biosciences
目录: LS-C263950
domestic rabbit 多克隆
反应物种: 人类
共轭标签: PE
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
NANOG抗体(aa94-123, AP)LS-C263951
LifeSpan Biosciences
目录: LS-C263951
domestic rabbit 多克隆
反应物种: 人类
共轭标签: AP
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪
规格:
价格:
至产商
NANOG抗体(aa94-123, APC)LS-C263952
LifeSpan Biosciences
目录: LS-C263952
domestic rabbit 多克隆
反应物种: 人类
共轭标签: APC
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪
规格:
价格:
至产商
NANOG抗体(aa94-123, 异硫氰酸荧光素)LS-C263953
LifeSpan Biosciences
目录: LS-C263953
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪
规格:
价格:
至产商
NANOG抗体(aa94-123, 无叠氮物, HRP)LS-C263954
LifeSpan Biosciences
目录: LS-C263954
domestic rabbit 多克隆
反应物种: 人类
共轭标签: HRP
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪
规格:
价格:
至产商
NANOG抗体(aa94-123, PE)LS-C263957
LifeSpan Biosciences
目录: LS-C263957
domestic rabbit 多克隆
反应物种: 人类
共轭标签: PE
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪
规格:
价格:
至产商
NANOG抗体(磷酸化Ser71, AP)LS-C279943
LifeSpan Biosciences
目录: LS-C279943
domestic rabbit 多克隆
反应物种: 人类
共轭标签: AP
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(磷酸化Ser71, APC)LS-C279944
LifeSpan Biosciences
目录: LS-C279944
domestic rabbit 多克隆
反应物种: 人类
共轭标签: APC
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(磷酸化Ser71, 异硫氰酸荧光素)LS-C279945
LifeSpan Biosciences
目录: LS-C279945
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(磷酸化Ser71, 无叠氮物, HRP)LS-C279946
LifeSpan Biosciences
目录: LS-C279946
domestic rabbit 多克隆
反应物种: 人类
共轭标签: HRP
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(磷酸化Ser71, PE)LS-C279947
LifeSpan Biosciences
目录: LS-C279947
domestic rabbit 多克隆
反应物种: 人类
共轭标签: PE
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(磷酸化Ser285, AP)LS-C279948
LifeSpan Biosciences
目录: LS-C279948
domestic rabbit 多克隆
反应物种: 人类
共轭标签: AP
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(磷酸化Ser285, APC)LS-C279949
LifeSpan Biosciences
目录: LS-C279949
domestic rabbit 多克隆
反应物种: 人类
共轭标签: APC
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(磷酸化Ser285, 异硫氰酸荧光素)LS-C279950
LifeSpan Biosciences
目录: LS-C279950
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(磷酸化Ser285, 无叠氮物, HRP)LS-C279951
LifeSpan Biosciences
目录: LS-C279951
domestic rabbit 多克隆
反应物种: 人类
共轭标签: HRP
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(磷酸化Ser285, PE)LS-C279952
LifeSpan Biosciences
目录: LS-C279952
domestic rabbit 多克隆
反应物种: 人类
共轭标签: PE
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(aa1-50)LS-C286456
LifeSpan Biosciences
目录: LS-C286456
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫组化
规格:
价格:
至产商
NANOG抗体(aa250-C-terminal)LS-C287289
LifeSpan Biosciences
目录: LS-C287289
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫沉淀
规格:
价格:
至产商
NANOG抗体(aa1-50)LS-C287290
LifeSpan Biosciences
目录: LS-C287290
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹, 免疫沉淀
规格:
价格:
至产商
NANOG抗体(Ser285)LS-C323224
LifeSpan Biosciences
目录: LS-C323224
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学
规格:
价格:
至产商
NANOG抗体(aa94-123)LS-C323228
LifeSpan Biosciences
目录: LS-C323228
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪
规格:
价格:
至产商
NANOG抗体(磷酸化Ser71)LS-C327996
LifeSpan Biosciences
目录: LS-C327996
domestic rabbit 多克隆
反应物种: 人类
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(磷酸化Ser285)LS-C327997
LifeSpan Biosciences
目录: LS-C327997
domestic rabbit 多克隆
反应物种: 人类
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(克隆2C4)LS-C348865
LifeSpan Biosciences
目录: LS-C348865
小鼠 单克隆 (2C4)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪
规格:
价格:
至产商
NANOG抗体(aa155-189)LS-C356626
LifeSpan Biosciences
目录: LS-C356626
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

Anti-NANOG Antibody at 1:2000 dilution + human brain lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
规格:
价格:
至产商
NANOG抗体(aa20-166, 克隆1E6C4)LS-C359267
LifeSpan Biosciences
目录: LS-C359267
小鼠 单克隆 (1E6C4)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
NANOG抗体(aa1-305)LS-C374979
LifeSpan Biosciences
目录: LS-C374979
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

Western Blot; Lane1: Human Umbilical Cord Tissue; Lane2: Human Placenta Tissue.

规格:
价格:
至产商
NANOG抗体(异硫氰酸荧光素)LS-C380164
LifeSpan Biosciences
目录: LS-C380164
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体LS-C380166
LifeSpan Biosciences
目录: LS-C380166
domestic rabbit 多克隆
反应物种: 人类
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(HRP)LS-C380168
LifeSpan Biosciences
目录: LS-C380168
domestic rabbit 多克隆
反应物种: 人类
共轭标签: HRP
应用: 酶联免疫吸附测定
规格:
价格:
至产商
NANOG抗体(生物素)LS-C380170
LifeSpan Biosciences
目录: LS-C380170
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 生物素
应用: 酶联免疫吸附测定
规格:
价格:
至产商