IHC-plus™ KLF4 Antibody (aa1-170, clone AT4E6) LS-B2966
LifeSpan Biosciences
目录: LS-B2966
小鼠 单克隆 (AT4E6)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片

Anti-KLF4 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.

Cell lysates of HeLa and A549 (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human KLF4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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IHC-plus™ KLF4 Antibody (aa80-129) LS-B1192
LifeSpan Biosciences
目录: LS-B1192
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片

Anti-KLF4 antibody IHC of human melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC-plus™ KLF4 Antibody (aa401-450) LS-B5641
LifeSpan Biosciences
目录: LS-B5641
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫组化-石蜡切片

Anti-KLF4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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KLF4抗体(aa28-38)LS-C8370
LifeSpan Biosciences
目录: LS-C8370
domestic rabbit 多克隆
反应物种: 人类
应用: 酶联免疫吸附测定
规格:
价格:
至产商
KLF4抗体(aa11-22)LS-C55037
LifeSpan Biosciences
目录: LS-C55037
domestic goat 多克隆
反应物种: 人类
应用: 免疫印迹

Antibody (0.1 ug/ml) staining of Human Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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KLF4抗体(克隆56CT5.1。 6)LS-C96469
LifeSpan Biosciences
目录: LS-C96469
小鼠 单克隆 (56CT5.1.6)
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪

Western blot of lysates with KLF4 protein, using KLF4 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

Flow cytometric of MCF-7 cells using KLF4 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Immunofluorescence of KLF4 antibody (5 ug/ml). HeLa cells transfected with pMX constructs of human KLF4 (top) and NIH3T3 cells transfected with pMX constructs of mouse KLF4 (bottom) were analyzed at approximately 62 hours after transfection.
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KLF4抗体(aa69-101)LS-C98588
LifeSpan Biosciences
目录: LS-C98588
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片

Western blot of KLF4 Antibody (N-term C74) in Jurkat,293 cell line lysates (35 ug/lane). KLF4 (arrow) was detected using the purified antibody.

Fluorescent confocal image of HeLa cells stained KLF4 (N-term C74) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 (N-term C74) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). KLF4 immunoreactivity is localized to the nuclei in HeLa cells.

Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with KLF4 antibody (N-term C74) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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KLF4抗体LS-C98590
LifeSpan Biosciences
目录: LS-C98590
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片

Western blot of lysate from HT29 cell line, using KLF4 Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Fluorescent confocal image of HeLa cells stained KLF4 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). KLF4 immunoreactivity is localized specifically to the nuclei in HeLa cells.

Formalin-fixed and paraffin-embedded human colon carcinoma with KLF4 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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KLF4抗体(Ser457)LS-C98591
LifeSpan Biosciences
目录: LS-C98591
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学

Fluorescent confocal image of HeLa cells stained KLF4 (S457) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 (S457) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei but not the cytoplasm.

Western blot of KLF4-S457 in MDA-MB231, NCI-H460 cell line lysates (35 ug/lane). KLF4 (arrow) was detected using the purified antibody.
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KLF4抗体LS-C108660
LifeSpan Biosciences
目录: LS-C108660
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化

Immunohistochemistry of KLF4 in rat liver tissue with KLF4 antibody at 5 ug/ml.

Western blot analysis of KLF4 in human liver tissue lysate with KLF4 antibody at (A) 1 and (B) 2 ug/ml.
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KLF4抗体(aa251-300)LS-C116050
LifeSpan Biosciences
目录: LS-C116050
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹
规格:
价格:
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KLF4抗体(N末端)LS-C135057
LifeSpan Biosciences
目录: LS-C135057
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹
规格:
价格:
至产商
KLF4抗体LS-C136894
LifeSpan Biosciences
目录: LS-C136894
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
规格:
价格:
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KLF4抗体(克隆4G6E11)LS-C144495
LifeSpan Biosciences
目录: LS-C144495
小鼠 单克隆 (4G6E11)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定

Western blot analysis of KLF4 in mouse liver tissue lysate with KLF4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
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KLF4抗体(克隆4E5C3)LS-C144496
LifeSpan Biosciences
目录: LS-C144496
小鼠 单克隆 (4E5C3)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定

Western blot analysis of KLF4 in mouse liver tissue lysate with KLF4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
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KLF4抗体LS-C146849
LifeSpan Biosciences
目录: LS-C146849
domestic rabbit 多克隆
反应物种: 人类, 大鼠
应用: 免疫印迹
规格:
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KLF4抗体(aa394-421)LS-C165557
LifeSpan Biosciences
目录: LS-C165557
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 免疫细胞化学, 流式细胞仪

KLF4 Antibody western blot of A431,MCF-7 cell line lysates (35 ug/lane). The KLF4 antibody detected the KLF4 protein (arrow).

Fluorescent confocal image of SY5Y cells stained KLF4 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated KLF4 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). KLF4 immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.

KLF4 Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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KLF4抗体(克隆1E5)LS-C169232
LifeSpan Biosciences
目录: LS-C169232
小鼠 单克隆 (1E5)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化
规格:
价格:
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KLF4抗体(克隆1E6)LS-C169255
LifeSpan Biosciences
目录: LS-C169255
小鼠 单克隆 (1E6)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学
规格:
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KLF4抗体(aa283-314)LS-C203673
LifeSpan Biosciences
目录: LS-C203673
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

KLF4 Antibody western blot of MCF-7,K562 cell line lysates (35 ug/lane). The KLF4 antibody detected the KLF4 protein (arrow).
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KLF4抗体(克隆56CT5.1。 6, AP)LS-C250279
LifeSpan Biosciences
目录: LS-C250279
小鼠 单克隆 (56CT5.1.6)
反应物种: 人类
共轭标签: AP
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
规格:
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KLF4抗体(克隆56CT5.1。 6, APC)LS-C250280
LifeSpan Biosciences
目录: LS-C250280
小鼠 单克隆 (56CT5.1.6)
反应物种: 人类
共轭标签: APC
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
规格:
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KLF4抗体(克隆56CT5.1。 6, 生物素)LS-C250281
LifeSpan Biosciences
目录: LS-C250281
小鼠 单克隆 (56CT5.1.6)
反应物种: 人类
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
规格:
价格:
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KLF4抗体(克隆56CT5.1。 6, 异硫氰酸荧光素)LS-C250282
LifeSpan Biosciences
目录: LS-C250282
小鼠 单克隆 (56CT5.1.6)
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
规格:
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KLF4抗体(克隆56CT5.1。 6, PE)LS-C250285
LifeSpan Biosciences
目录: LS-C250285
小鼠 单克隆 (56CT5.1.6)
反应物种: 人类
共轭标签: PE
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
规格:
价格:
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KLF4抗体(克隆56CT5.1。 6, 无叠氮物, HRP)LS-C250286
LifeSpan Biosciences
目录: LS-C250286
小鼠 单克隆 (56CT5.1.6)
反应物种: 人类
共轭标签: HRP
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
规格:
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KLF4抗体(Ser457, APC)LS-C261419
LifeSpan Biosciences
目录: LS-C261419
domestic rabbit 多克隆
反应物种: 人类
共轭标签: APC
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
KLF4抗体(Ser457, AP)LS-C261420
LifeSpan Biosciences
目录: LS-C261420
domestic rabbit 多克隆
反应物种: 人类
共轭标签: AP
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
KLF4抗体(Ser457, 异硫氰酸荧光素)LS-C261421
LifeSpan Biosciences
目录: LS-C261421
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
KLF4抗体(Ser457, 生物素)LS-C261422
LifeSpan Biosciences
目录: LS-C261422
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 生物素
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
KLF4抗体(Ser457, 无叠氮物, HRP)LS-C261423
LifeSpan Biosciences
目录: LS-C261423
domestic rabbit 多克隆
反应物种: 人类
共轭标签: HRP
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
KLF4抗体(Ser457, PE)LS-C261424
LifeSpan Biosciences
目录: LS-C261424
domestic rabbit 多克隆
反应物种: 人类
共轭标签: PE
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
KLF4抗体(磷酸化Ser245, AP)LS-C261425
LifeSpan Biosciences
目录: LS-C261425
domestic rabbit 多克隆
反应物种: 人类
共轭标签: AP
应用: 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
KLF4抗体(磷酸化Ser245, APC)LS-C261426
LifeSpan Biosciences
目录: LS-C261426
domestic rabbit 多克隆
反应物种: 人类
共轭标签: APC
应用: 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
KLF4抗体(磷酸化Ser245, 异硫氰酸荧光素)LS-C261427
LifeSpan Biosciences
目录: LS-C261427
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 异硫氰酸荧光素
应用: 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
KLF4抗体(磷酸化Ser245, 生物素)LS-C261428
LifeSpan Biosciences
目录: LS-C261428
domestic rabbit 多克隆
反应物种: 人类
共轭标签: 生物素
应用: 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
KLF4抗体(磷酸化Ser245, 无叠氮物, HRP)LS-C261429
LifeSpan Biosciences
目录: LS-C261429
domestic rabbit 多克隆
反应物种: 人类
共轭标签: HRP
应用: 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
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KLF4抗体(磷酸化Ser245, PE)LS-C261430
LifeSpan Biosciences
目录: LS-C261430
domestic rabbit 多克隆
反应物种: 人类
共轭标签: PE
应用: 酶联免疫吸附测定, 免疫细胞化学
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价格:
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KLF4抗体(aa93-108)LS-C313313
LifeSpan Biosciences
目录: LS-C313313
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

WB of KLF4 antibody. Lane 1: HELA Cell Lysate. Lane 2: A549 Cell Lysate. Lane 3: U20S Cell Lysate.

KLF4 antibody. IHC(P): Human Tonsil Tissue.

KLF4 antibody. ICC: A549 Cell.
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KLF4抗体(克隆56CT5.1。 6)LS-C319168
LifeSpan Biosciences
目录: LS-C319168
小鼠 单克隆 (56CT5.1.6)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪
规格:
价格:
至产商
KLF4抗体(磷酸化Ser245)LS-C322512
LifeSpan Biosciences
目录: LS-C322512
domestic rabbit 多克隆
反应物种: 人类
应用: 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
KLF4抗体(Ser457)LS-C322514
LifeSpan Biosciences
目录: LS-C322514
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
规格:
价格:
至产商
KLF4抗体(aa1-50)LS-C47390
LifeSpan Biosciences
目录: LS-C47390
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
规格:
价格:
至产商
KLF4抗体(aa408-450)LS-C356334
LifeSpan Biosciences
目录: LS-C356334
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹

Western blot of lysate from NCCIT cell line, using KLF4 antibody diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
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KLF4抗体(aa321-354)LS-C356421
LifeSpan Biosciences
目录: LS-C356421
domestic rabbit 多克隆
反应物种: 人类, 小鼠
应用: 免疫印迹

Western blot of lysate from mouse heart tissue lysate, using Klf4 antibody diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
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KLF4抗体(N末端)LS-C357947
LifeSpan Biosciences
目录: LS-C357947
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
规格:
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至产商
KLF4抗体(aa1-300)LS-C358155
LifeSpan Biosciences
目录: LS-C358155
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
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至产商
KLF4抗体(aa30-230)LS-C376030
LifeSpan Biosciences
目录: LS-C376030
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片

Western blot. All lanes: Krueppel-like factor 4 antibody at 5 ug/ml+293T whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 55 kDa. Observed band size: 55 kDa Immunohistochemistry.

Immunohistochemistry of paraffin-embedded human rectum using antibody at 1:100 dilution.
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