抗体

IHC-plus™ MDM2 Antibody (aa154-167, clone SMP14) LS-B163

LS-B163

单克隆

小鼠

未共轭

SMP14

免疫组化, 免疫组化-石蜡切片, 免疫组化-冰冻切片

LS-B163

人类

小鼠

IHC-plus™ MDM2 Antibody (aa154-167, clone SMP14) LS-B163

Recognizes the 90kD MDM2 protein, which forms a complex with p53 leading to p53 inhibition. MDM2 also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI. MDM2 is over expressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer.

单克隆

SMP14

未共轭

aa154-167

PBS, 0.09% 叠氮化钠

合成肽

Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2. Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Rat, Dog, Panda (100%); Bat, Cat, Bovine, Hamster, Elephant, Horse, Pig (93%); Mouse (86%).

纯化IgG

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

IgG1

鼠双微基因2

鼠双微基因2

人类

IHC, IHC-Fr, IHC-P (10 µg/ml), IHC-R

MDM2 antibody LS-B163 is an unconjugated mouse monoclonal antibody to human MDM2 (aa154-167). Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Immunohistochemistry: LS-B163 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B163 was determined to be 10 ug/ml. Histology Positive Control Tissue: Breast Carcinoma.

MDM2, ACTFS, Double minute 2 protein, Hdm2, MDM2 variant FB30, HDMX, Oncoprotein Mdm2, p53-binding protein Mdm2, MDM2 variant FB28

Worldwide