抗体

IHC-plus™ TP73 / p73 Antibody (N-Terminus, clone 5B429) LS-B2086

LS-B2086

单克隆

小鼠

未共轭

5B429

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片

LS-B2086

人类

小鼠

IHC-plus™ TP73 / p73 Antibody (N-Terminus, clone 5B429) LS-B2086

Recognizes all of the TAp73 isoforms i. e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kD and 45 kD, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature.

单克隆

5B429

未共轭

N末端

PBS, 0.05% 叠氮化钠

Full-length human p73. It reacts with an epitope that is located on the N-Terminus of human p73. It does not cross react with p53.

蛋白质G纯化

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

IgG

TP73/p73

TP73

小鼠, 人类

免疫细胞化学, 免疫组化, 免疫组化-P(5微克/毫升), 免疫沉淀, 免疫印迹(2微克/毫升)

P73 antibody LS-B2086 is an unconjugated mouse monoclonal antibody to p73 (TP73) (N-Terminus) from human. It is reactive with human and mouse. Validated for ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Immunohistochemistry: LS-B2086 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2086 was determined to be 5 ug/ml.

TP73, p53-like transcription factor, p73, p53-related protein, Tumor protein p73

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