产品简要
公司名称 :
LifeSpan Biosciences
产品类型 :
抗体
产品名称 :
IHC-plus™ OCLN / Occludin Antibody (C-Terminus, clone OC-3F10) LS-B2320
目录 :
LS-B2320
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
OC-3F10
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片
更多信息或购买 :
LifeSpan Biosciences 产品网页
文章摘录数: 2
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 1:2000; 图 7b
Bendriem R, Singh S, Aleem A, Antonetti D, Ross M. Tight junction protein occludin regulates progenitor Self-Renewal and survival in developing cortex. elife. 2019;8: pubmed 出版商
Otsu K, Ida Yonemochi H, Fujiwara N, Harada H. The Semaphorin 4D-RhoA-Akt Signal Cascade Regulates Enamel Matrix Secretion in Coordination With Cell Polarization During Ameloblast Differentiation. J Bone Miner Res. 2016;31:1943-1954 pubmed 出版商
图像
图像 1 :
LifeSpan Biosciences LS-B2320 图像 1
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
产品信息
抗体编号 :
82908
抗体名称 :
LS-B2320
目标物种 :
人类
宿主物种 :
小鼠
产品名称 :
IHC-plus™ OCLN / Occludin Antibody (C-Terminus, clone OC-3F10) LS-B2320
特异性 :
Recognizes mammalian Occludin Protein. Species cross-reactivity: human, mouse, rat and canine.
克隆描述 :
单克隆
克隆名称 :
OC-3F10
抗体修饰 :
未共轭
抗原修饰 :
C末端
样品状态 :
PBS, pH值7.4, 0.09% 叠氮化钠
免疫原类型 :
融合蛋白
免疫原描述 :
GST fusion protein consisting of the C-Terminus (~150aa) of human occludin.
纯化描述 :
蛋白质A纯化
推荐的储存条件 :
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
抗体亚型 :
IgG1, k
基因 :
OCLN / Occludin
标准基因符号 :
OCLN
反应物种 :
小鼠, 狗, 大鼠, 人类
用途 :
酶联免疫吸附测定(0.1-1微克/毫升), IF(2-3微克/毫升), 免疫组化, 免疫组化-P(5-10微克/毫升), 免疫印迹(0.1-1微克/毫升)
ShortWebDescription :
Occludin antibody LS-B2320 is an unconjugated mouse monoclonal antibody to Occludin (OCLN) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
使用文本 :
Immunohistochemistry: LS-B2320 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2320 was determined to be 10 ug/ml. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma). Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells.
同义词 :
OCLN, Occludin, BLCPMG
SalesRegion :
Worldwide
更多信息或购买 :
LifeSpan Biosciences 产品网页
公司信息
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
公司总部: 美国
LifeSpan成立于1995年，已经成为分子病理学领域的领导品牌，专注于人和其他物种的正常和疾病组织中蛋白的定位情况。我们提供超过74000种抗体，定制的免疫组化研究服务，超过500种蛋白研究用人免疫组化材料，及组化和病理学服务；提供2,000,000样本，包括冰冻及石蜡切片的正常和疾病组织，进行对外研究服务。对外服务项目范围广泛，由LifeSpan的病理学家提供包括课题设计、抗体搜寻及表征、组织搜集和验证、免疫标记、问题处理及结果处理等诸多环节。