抗体

IHC-plus™ PAX2 Antibody (phospho-Ser393) LS-B2450

LS-B2450

多克隆

domestic rabbit

未共轭

磷酸化

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片

LS-B2450

人类

兔

IHC-plus™ PAX2 Antibody (phospho-Ser393) LS-B2450

Recognizes human Pax2 protein when phosphorylated at Ser393. Based on amino acid sequence homology with Pax5 and Pax8, cross-reactivity with these proteins is likely. Does not react with purified, unphosphorylated GST-Pax2 proteins. Reacts with human HEK293 cell lysates and purified GST-Pax2 proteins phosphorylated by JNK. Species sequence Homology: mouse, chicken, zebra sh and frog - 100%.

多克隆

未共轭

pSer393

PBS, pH值7.4, 0.1% 叠氮化钠

Synthetic phospho-peptide

Synthetic phospho-peptide derived from the sequence surrounding the phosphorylated Ser393 residue of mouse and human Pax2 (paired box protein 2).

Immunoaffinity purified

存放在4°C或-20°C。 避免冻融循环

IgG

PAX2

PAX2

人类

酶联免疫吸附测定(0.1-1微克/毫升), 免疫组化, 免疫组化-P(1:50), 免疫印迹(1-3微克/毫升)

PAX2 antibody LS-B2450 is an unconjugated rabbit polyclonal antibody to human PAX2 (pSer393). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Immunohistochemistry: LS-B2450 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2450 was determined to be 1:50. Western Blot: Analysis of JNK-stimulated (phosphorylated) fusion proteins identifies a single band at ~50kD. In Western Blot analysis of HEK293 cells, a thick band between ~42-45kD is observed, which may represent different human Pax2 isoforms and/or Pax8.

PAX2, Paired box homeotic gene 2, Paired box protein Pax-2, PAPRS, Paired box 2, Paired box gene 2

Worldwide