抗体

PathPlus™ TH / Tyrosine Hydroxylase Antibody (phospho-Ser40) LS-B2462

LS-B2462

多克隆

domestic rabbit

未共轭

磷酸化

人类, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片, 免疫组化-冰冻切片

LS-B2462

大鼠

兔

PathPlus™ TH / Tyrosine Hydroxylase Antibody (phospho-Ser40) LS-B2462

Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.

多克隆

未共轭

pSer40

10 mM羟乙基哌嗪乙磺酸, pH值7.5, 150 mM氯化钠, 0.1毫克/毫升BSA, 50% 甘油

A phosphopeptide corresponding to amino acid residues surrounding the phosphoSer40 of rat tyrosine hydroxylase

Immunoaffinity purified

Short term: -20°C; Long term: -80°C.

TH/酪氨酸羟化酶

TH

Monooxygenase

大鼠, 人类

IF (1:1000), IHC, IHC-Fr (1:1000), IHC-P (5 µg/ml), WB (1:1000)

Tyrosine Hydroxylase antibody LS-B2462 is an unconjugated rabbit polyclonal antibody to Tyrosine Hydroxylase (TH) (pSer40) from rat. It is reactive with human and rat. Validated for IF, IHC and WB.

Immunohistochemistry: LS-B2462 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2462 was determined to be 5 ug/ml.

TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase

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