抗体

IHC-plus™ MMP9 / Gelatinase B Antibody LS-B2486

LS-B2486

多克隆

domestic rabbit

未共轭

人类, 大鼠

免疫印迹, 免疫组化, 免疫沉淀, 免疫组化-石蜡切片, 免疫组化-冰冻切片

LS-B2486

大鼠

兔

IHC-plus™ MMP9 / Gelatinase B Antibody LS-B2486

Recognizes rat MMP-9 pro and active forms. Exhibits no cross-reactivity with other MMP family members.

多克隆

未共轭

PBS, 0.05% 叠氮化钠

重组蛋白

E. coli expressed active rat 92 kDa type IV collagenase (catalytic domain)

蛋白质A纯化

Short term: 4°C Long term: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

IgG

基质金属蛋白酶9/明胶酶B

基质金属蛋白酶9

蛋白酶

金属肽酶M10A

小鼠, 大鼠, 人类

IHC, IHC-Fr (1:50), IHC-P (5 µg/ml), IP (1:250), WB (1:1000)

Gelatinase B antibody LS-B2486 is an unconjugated rabbit polyclonal antibody to Gelatinase B (MMP9) from rat. It is reactive with human, mouse and rat. Validated for IHC, IP and WB. Cited in 4 publications.

Immunohistochemistry: LS-B2486 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2486 was determined to be 5 ug/ml. Western Blot: 1:500. Under fully reduced conditions, typically 98-95kD pro and 92-88kD active forms and breakdown products are detected. Under non-reduced conditions, additional bands may be detected due to SDS-stable TIMP and other inhibitor complexes. Positive control: HL660 cell lysate, U937 cell lysate, lung.

基质金属蛋白酶9, 92kd明胶酶, 92kDa的明胶酶, 基质金属蛋白酶9, 基质金属蛋白酶-9, CLG4B, 明胶酶B, GELB, 巨噬细胞明胶酶, MANDP2

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