产品简要
公司名称 :
LifeSpan Biosciences
产品类型 :
抗体
产品名称 :
IHC-plus™ IL7R / CD127 Antibody (phospho-Tyr449) LS-B2831
目录 :
LS-B2831
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
抗原修饰 :
磷酸化
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片
更多信息或购买 :
LifeSpan Biosciences 产品网页
图像
图像 1 :
LifeSpan Biosciences LS-B2831 图像 1
Anti-IL-7 Receptor antibody IHC of human colon, MALT. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
产品信息
抗体编号 :
90088
抗体名称 :
LS-B2831
目标物种 :
小鼠
宿主物种 :
兔
产品名称 :
IHC-plus™ IL7R / CD127 Antibody (phospho-Tyr449) LS-B2831
特异性 :
This antibody is specific for mouse IL-7 protein phosphorylated at Y449. A BLAST analysis was used to suggest cross-reactivity with IL-7 from human, mouse and rat sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-7 from other sources has not been determined.
克隆描述 :
多克隆
抗体修饰 :
未共轭
抗原修饰 :
pTyr449
样品状态 :
0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.01% 叠氮化钠
免疫原类型 :
合成肽
免疫原描述 :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.
纯化描述 :
亲和纯化
推荐的储存条件 :
Product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
抗体亚型 :
IgG
基因 :
IL7R/CD127
标准基因符号 :
IL7R
家族 :
白细胞介素
反应物种 :
小鼠, 大鼠, 人类
用途 :
酶联免疫吸附测定(1:5000 - 1:50000), 免疫组化, 免疫组化-P(5微克/毫升), 免疫印迹(1:10000)
ShortWebDescription :
CD127 antibody LS-B2831 is an unconjugated rabbit polyclonal antibody to CD127 (IL7R) (pTyr449) from mouse. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
使用文本 :
Immunohistochemistry: LS-B2831 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2831 was determined to be 5 ug/ml. Western Blot: Expect a band approximately 55 kD in size corresponding to phosphorylated IL-7 in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
同义词 :
IL7R, CD127, CD127抗原, IL-7RA, IL-7受体亚单位甲, IL7RA, ILRA, 白细胞介素-7受体, CDW127, IL-7R亚单位甲, IL-7R-alpha
SalesRegion :
Worldwide
更多信息或购买 :
LifeSpan Biosciences 产品网页
公司信息
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
公司总部: 美国
LifeSpan成立于1995年，已经成为分子病理学领域的领导品牌，专注于人和其他物种的正常和疾病组织中蛋白的定位情况。我们提供超过74000种抗体，定制的免疫组化研究服务，超过500种蛋白研究用人免疫组化材料，及组化和病理学服务；提供2,000,000样本，包括冰冻及石蜡切片的正常和疾病组织，进行对外研究服务。对外服务项目范围广泛，由LifeSpan的病理学家提供包括课题设计、抗体搜寻及表征、组织搜集和验证、免疫标记、问题处理及结果处理等诸多环节。