抗体

IHC-plus™ Collagen VI Antibody LS-B3771

LS-B3771

多克隆

domestic goat

未共轭

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LS-B3771

人类

山羊

IHC-plus™ Collagen VI Antibody LS-B3771

Reacts with conformational determinants on bovine and human type VI collagen as demonstrated by ELISA. May react with type VI collagen from other species. Exhibits < 10% cross-reactivity with collagen type I, II, III, IV and V. Has not been tested for reactivity with other ECM proteins (e. g., laminin, fibronectin).

多克隆

未共轭

Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.

纯化蛋白

人类Ⅵ型胶原

Immunoaffinity purified

存放在4°C或-20°C。 避免冻融循环

胶原VI

人类

DB, ELISA, ICC (1:10 - 1:20), IHC, IHC-Fr, IHC-P (10 µg/ml)

Collagen VI antibody LS-B3771 is an unconjugated goat polyclonal antibody to human Collagen VI. Validated for DB, ELISA, ICC and IHC. Tested on 20 paraffin-embedded human tissues.

Immunohistochemistry: LS-B3771 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3771 was determined to be 10 ug/ml.

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