抗体

IHC-plus™ FN1 / Fibronectin Antibody LS-B382

LS-B382

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫沉淀, 免疫组化-石蜡切片

LS-B382

人类

兔

IHC-plus™ FN1 / Fibronectin Antibody LS-B382

Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.

多克隆

未共轭

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.01% 叠氮化钠

纯化蛋白

Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine.  The eluted material was further purified by gel filtration.  Immunization occurred after single-band purity was assessed by SDS-PAGE.

亲和色谱

Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.

IgG

FN1/纤连蛋白

FN1

人类

酶联免疫吸附测定(1:5000 - 1:10000), 免疫组化, 免疫组化-P(1.25微克/毫升), 免疫沉淀(1:100), 免疫印迹(1:5000 - 1:10000)

Fibronectin antibody LS-B382 is an unconjugated rabbit polyclonal antibody to human Fibronectin (FN1). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Immunohistochemistry: LS-B382 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B382 was determined to be 1.25 ug/ml.

FN1, CIG, Cold-insoluble globulin, Fibronectin 1, FINC, LETS, GFND, ED-B, Fibronectin, FN, FNZ, GFND2, Migration-stimulating factor, MSF

Worldwide