产品简要
公司名称 :
LifeSpan Biosciences
产品类型 :
抗体
产品名称 :
PathPlus™ HSPB1 / HSP27 Antibody (clone G3.1) LS-B383
目录 :
LS-B383
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
G3.1
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片
更多信息或购买 :
LifeSpan Biosciences 产品网页
文章摘录数: 3
参考文献
Edwards D, Adams D, McGuire W. Estradiol stimulates synthesis of a major intracellular protein in a human breast cancer cell line (MCF-7). Breast Cancer Res Treat. 1981;1:209-23 pubmed
Moretti Rojas I, Fuqua S, Montgomery R, McGuire W. A cDNA for the estradiol-regulated 24K protein: control of mRNA levels in MCF-7 cells. Breast Cancer Res Treat. 1988;11:155-63 pubmed
McGuire S, Fuqua S, Naylor S, Helin Davis D, McGuire W. Chromosomal assignments of human 27-kDa heat shock protein gene family. Somat Cell Mol Genet. 1989;15:167-71 pubmed
图像
图像 1 :
LifeSpan Biosciences LS-B383 图像 1
Anti-HSPB1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
产品信息
抗体编号 :
31342
抗体名称 :
LS-B383
目标物种 :
人类
宿主物种 :
小鼠
产品名称 :
PathPlus™ HSPB1 / HSP27 Antibody (clone G3.1) LS-B383
特异性 :
Reacts specifically with HSP27 in human tissues and cell lines. MCF-7 cells are recommended as a positive control. Cross reactivity with hsp27 from other mammalian sources is likely. No cross reactivity occurs with HSP70, HSP90 or HSP104.
克隆描述 :
单克隆
克隆名称 :
G3.1
抗体修饰 :
未共轭
样品状态 :
0.02 M钾磷酸盐, pH值7.2, 0.5 M氯化钠, 0.01% 叠氮化钠
免疫原类型 :
重组蛋白
免疫原描述 :
This HSP27 monoclonal antibody was produced by repeated immunizations with a prokaryotic recombinant protein corresponding to the full length human hsp27 protein.
纯化描述 :
蛋白质A纯化
推荐的储存条件 :
存放在4°C或-20°C。 避免冻融循环
抗体亚型 :
IgG1, k
基因 :
HSPB1/HSP27
标准基因符号 :
HSPB1
反应物种 :
人类
用途 :
酶联免疫吸附测定(1:10000 - 1:50000), 免疫组化, 免疫组化-P(1.25微克/毫升), 免疫印迹(1:500 - 1:1000)
ShortWebDescription :
HSP27 antibody LS-B383 is an unconjugated mouse monoclonal antibody to human HSP27 (HSPB1). Validated for ELISA, IHC and WB. Cited in 3 publications.
使用文本 :
Immunohistochemistry: LS-B383 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B383 was determined to be 1.25 ug/ml.
同义词 :
HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067
SalesRegion :
Worldwide
更多信息或购买 :
LifeSpan Biosciences 产品网页
公司信息
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com
1-206-464-1554
公司总部: 美国
LifeSpan成立于1995年，已经成为分子病理学领域的领导品牌，专注于人和其他物种的正常和疾病组织中蛋白的定位情况。我们提供超过74000种抗体，定制的免疫组化研究服务，超过500种蛋白研究用人免疫组化材料，及组化和病理学服务；提供2,000,000样本，包括冰冻及石蜡切片的正常和疾病组织，进行对外研究服务。对外服务项目范围广泛，由LifeSpan的病理学家提供包括课题设计、抗体搜寻及表征、组织搜集和验证、免疫标记、问题处理及结果处理等诸多环节。