抗体

IHC-plus™ PCNA Antibody LS-B402

LS-B402

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片

LS-B402

人类

兔

IHC-plus™ PCNA Antibody LS-B402

A BLAST analysis was used to suggest cross reactivity with PCNA protein from human, monkey, dog, mouse, rat, bovine, chicken, fish and Xenopus based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

多克隆

未共轭

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.01% 叠氮化钠

合成肽

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PCNA protein.

亲和纯化

Aliquot and store at -20°C or below for extended storage. Avoid freeze / thaw cycles. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature.

IgG

增殖细胞核抗原

增殖细胞核抗原

Xenopus, Chicken, Fish, Rat, Mouse, Dog, Bovine, Human, Monkey

酶联免疫吸附测定(1:2000 - 1:8000), 免疫组化, 免疫组化-P(2.5微克/毫升), 免疫印迹(1:500 - 1:2000)

PCNA antibody LS-B402 is an unconjugated rabbit polyclonal antibody to PCNA from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Immunohistochemistry: LS-B402 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B402 was determined to be 2.5 ug/ml.

增殖细胞核抗原, 细胞周期蛋白

Worldwide