抗体

IHC-plus™ VDAC1 / PORIN Antibody LS-B435

LS-B435

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片

LS-B435

人类

兔

IHC-plus™ VDAC1 / PORIN Antibody LS-B435

A BLAST analysis was used to suggest that this antibody would react with VDAC1/Porin1 from a wide range or organisms, including avian, mammalian, aquatic and reptilian sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3 (2 isoforms). Such broad reactivity makes this antibody useful as an excellent loading control (mitochondrial).

多克隆

未共轭

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.01% 叠氮化钠

合成肽

VDAC/Porin Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 175-200 of Human VDAC1/Porin1.

亲和纯化

存放在4°C或-20°C。 避免冻融循环

IgG

VDAC1/孔蛋白

VDAC1

离子通道

VDAC孔蛋白

小鼠, 山羊, 牛, 大鼠, 猪, 人类

酶联免疫吸附测定(1:15000 - 1:30000), 免疫组化, 免疫组化-P(2.5微克/毫升), 免疫印迹(1:500 - 1:2000)

PORIN antibody LS-B435 is an unconjugated rabbit polyclonal antibody to PORIN (VDAC1) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Immunohistochemistry: LS-B435 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B435 was determined to be 2.5 ug/ml.

VDAC1, HVDAC1, Plasmalemmal porin, Porin 31HM, PORIN, PORIN-31-HL, Vdac5, VDAC, Mitochondrial porin, Porin 31HL, VDAC-1

Worldwide