抗体

IHC-plus™ MHC Class II RT-1B Antibody (clone OX-6) LS-B5258

LS-B5258

单克隆

小鼠

未共轭

OX-6

免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片

LS-B5258

大鼠

小鼠

IHC-plus™ MHC Class II RT-1B Antibody (clone OX-6) LS-B5258

Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Does not react with the BDIX rat strain. Expression is polymorphic in mice. This product is routinely tested in flow cytometry on rat splenocytes.

单克隆

OX-6

未共轭

PBS, 0.09% 叠氮化钠

Membrane extract

Rat thymocyte membrane glycoprotein.

蛋白质G纯化

Short term: store at 4°C. Long term: store at -20°C.

IgG1

MHC Class II RT-1B

大鼠

Flo (1:50 - 1:100), IF, IHC, IHC-Fr, IHC-P (10 µg/ml)

MHC Class II RT-1B antibody LS-B5258 is an unconjugated mouse monoclonal antibody to rat MHC Class II RT-1B. Validated for Flow, IF and IHC.

Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. Immunohistology: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH6.This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)

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