抗体

IHC-plus™ SMAD3 Antibody (phospho-Ser423/425) LS-B64

LS-B64

多克隆

domestic rabbit

未共轭

磷酸化

免疫印迹, 酶联免疫吸附测定, 免疫组化, 流式细胞仪, 免疫组化-石蜡切片

LS-B64

人类

兔

IHC-plus™ SMAD3 Antibody (phospho-Ser423/425) LS-B64

Reactivity occurs against human Smad3 pS423 and pS425 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Smad3 is minimal by ELISA and western blot. Expect reactivity against phosphorylated Smad1 and Smad5. Negligible reactivity is seen against other phosphorylated Smad family members. A BLAST analysis was used to suggest cross reactivity with Smad3 from human, Xenopus laevis, Xenopus tropicalis, zebrafish, rat, mouse, swine, bovine and chicken based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.

多克隆

未共轭

pSer423/425

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.01% 叠氮化钠

合成肽

Anti-SMAD3 pS423pS425 antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human SMAD3 protein.

亲和纯化

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

IgG

SMAD3

SMAD3

Xenopus laevis, Xenopus tropicalis, Chicken, Zebrafish, Pig, Bovine, Mouse, Rat, Human

ELISA (1:15000 - 1:30000), Flo, IHC, IHC-P (2.5 µg/ml), WB (1:2000 - 1:20000)

SMAD3 antibody LS-B64 is an unconjugated rabbit polyclonal antibody to SMAD3 (pSer423/425) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.

Immunohistochemistry: LS-B64 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B64 was determined to be 2.5 ug/ml.

SMAD3, HSPC193, HMAD-3, HsT17436, LDS1C, LDS3, Mad homolog JV15-2, MAD3, MADH3, Mothers against DPP homolog 3, JV15-2, SMA- and MAD-related protein 3, SMAD family member 3, MAD homolog 3, HSMAD3, Mad protein homolog, SMAD 3

Worldwide