抗体

PathPlus™ MTOR Antibody LS-B650

LS-B650

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫组化-石蜡切片

LS-B650

人类

兔

PathPlus™ MTOR Antibody LS-B650

A BLAST analysis was used to suggest cross reactivity with mTOR protein from rat and mouse based on 100% homology with the immunizing sequence. Expect partial reactivity against mTOR homologues from zebrafish (94%) and dog (89%). Reactivity against homologues from other sources is not known.

多克隆

未共轭

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.01% 叠氮化钠

合成肽

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 2430-2460 of human mTOR.

亲和纯化

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

IgG

哺乳动物雷帕霉素靶蛋白

哺乳动物雷帕霉素靶蛋白

蛋白激酶

PI3/PI4

小鼠, 大鼠, 人类

酶联免疫吸附测定(1:50000 - 1:100000), 免疫组化, 免疫组化-P(2.5微克/毫升), 免疫印迹(1:250 - 1:2000)

MTOR antibody LS-B650 is an unconjugated rabbit polyclonal antibody to MTOR from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.

Immunohistochemistry: LS-B650 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B650 was determined to be 2.5 ug/ml.

MTOR, FRAP1, FRAP2, FRAP, Mammalian target of rapamycin, RAFT1, Rapamycin and FKBP12 target 1, RAPT1, Rapamycin target protein 1

Worldwide