抗体

IHC-plus™ Alpha Tubulin Antibody (aa425-451) LS-B722

LS-B722

多克隆

domestic rabbit

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片

LS-B722

人类

兔

IHC-plus™ Alpha Tubulin Antibody (aa425-451) LS-B722

A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

多克隆

未共轭

aa425-451

0.02 M钾磷酸盐, pH值7.2, 0.15 M氯化钠, 0.01% 叠氮化钠

合成肽

Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.

亲和纯化

存放在4°C或-20°C。 避免冻融循环

IgG

α微管蛋白

鸡, 牛, 小鼠, 大鼠, 人类

酶联免疫吸附测定(1:90000), IF(1:500 - 1:2000), 免疫组化, 免疫组化-P(2.5微克/毫升), 免疫印迹(1:1000 - 1:5000)

Alpha Tubulin antibody LS-B722 is an unconjugated rabbit polyclonal antibody to Alpha Tubulin (aa425-451) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Immunohistochemistry: LS-B722 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B722 was determined to be 2.5 ug/ml.

Worldwide