产品简要
公司名称 :
LifeSpan Biosciences
产品类型 :
抗体
产品名称 :
CCL5/RANTES抗体LS-C104450
目录 :
LS-C104450
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定
更多信息或购买 :
产品信息
抗体编号 :
106300
抗体名称 :
LS-C104450
目标物种 :
人类
宿主物种 :
小鼠
产品名称 :
CCL5/RANTES抗体LS-C104450
特异性 :
人类RANTES
克隆描述 :
单克隆
抗体修饰 :
未共轭
样品状态 :
冻干来自PBS
免疫原类型 :
细胞提取物
免疫原描述 :
E.coli derived recombinant Human RANTES.
纯化描述 :
蛋白质A纯化
推荐的储存条件 :
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
重悬液体 :
Reconstitute with sterile water
抗体亚型 :
IgG2b
基因 :
CCL5/RANTES
标准基因符号 :
CCL5
家族 :
Intercrine
反应物种 :
人类
用途 :
酶联免疫吸附测定(2-4微克/毫升), 免疫印迹(0.5-1微克/毫升)
ShortWebDescription :
RANTES antibody LS-C104450 is an unconjugated mouse monoclonal antibody to human RANTES (CCL5). Validated for ELISA and WB.
使用文本 :
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 citrate buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. *Additional Immunostaining data available. Please contact Tech Support for information. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/ml), a concentration of 3-5 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 500 pg/ml of recombinant human RANTES when used with biotinylated antigen affinity purified anti-human RANTES (LS-C104720) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
同义词 :
CCL5, Chemokine (C-C motif) ligand 5, D17S136E, EoCP, T-cell specific protein p288, SCYA5, SISd, T cell-specific protein P228, Beta-chemokine RANTES, C-C motif chemokine 5, RANTES, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, TCP228
SalesRegion :
Worldwide
更多信息或购买 :
公司信息
LifeSpan Biosciences
2401 Fourth Avenue, Suite 900
Seattle, WA 98121
Seattle, WA 98121
antibodies@lsbio.com
https://www.lsbio.com1-206-464-1554
公司总部: 美国
LifeSpan成立于1995年，已经成为分子病理学领域的领导品牌，专注于人和其他物种的正常和疾病组织中蛋白的定位情况。我们提供超过74000种抗体，定制的免疫组化研究服务，超过500种蛋白研究用人免疫组化材料，及组化和病理学服务；提供2,000,000样本，包括冰冻及石蜡切片的正常和疾病组织，进行对外研究服务。对外服务项目范围广泛，由LifeSpan的病理学家提供包括课题设计、抗体搜寻及表征、组织搜集和验证、免疫标记、问题处理及结果处理等诸多环节。